Pharmacological preconditioning effect of isoflurane against renal ischemia/reperfusion injury in rats.

异氟烷对大鼠肾缺血/再灌注损伤的药理预处理作用。

• 批准号: 14571448
• 负责人: MOROOKA Hiroaki
• 金额: $ 2.18万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571448/
• 关键词: SAPK; JNK; p38 MAPK; Ischemia-Reperfusion; ERK; isoflurane; 薬理学的プレコンディショニング; 麻酔薬; 腎機能障害

Isoflurane had a pharmacological preconditioning effect in the heart and brain. In this study, we investigated about the kidney and the mechanism. Rats were divided into four groups (n=8 for each). In groups A and B were sham surgery. Groups C and D were subjected to 40-min bilateral renal ischemia. Groups B and D received 1.5 % isoflurane for 20 min before sham surgery or ischemia. Blood sampling was performed at 12,24 and 48 hrs after reperfusion. The values of serum creatinine and blood urea nitrogen showed significant increases in group C compared to D at 24 and 48 hrs (p<0.01). Additionally in the histopathological examination, suppression of the damage was seen with the isoflurane treatment. To investigate the mechanism, group I and II were subjected to 40-min unilateral renal ischemia. Group II received 1.5 % isoflurane for 20 min before ischemia. The activities of JNK, p38 and ERK were measured. The activities of JNK and ERK in group II were significantly lower than those in group I (p<0.05). In conclusion, isoflurane shows preconditioning effect against renal ischemia/reperfusion injury when administered before ischemia. Inhibition of the protein kinases, JNK and ERK, might be involved in the mechanisms.

异氟烷在心脏和大脑中具有药理预处理作用。在这项研究中，我们研究了肾脏和机制。将大鼠分为四组（每组n = 8）。在A组中，是假手术。 C组和D会接受40分钟的双侧肾脏缺血。 B和D组在假手术或缺血之前接受了1.5％的异氟烷​​20分钟。再灌注后12,24和48小时进行血液采样。血清肌酐和血液尿素氮的值在C组中显示出显着增加，而D当时为24和48小时（p <0.01）。另外，在组织病理学检查中，通过异氟烷处理可以看到损害的抑制。为了研究该机制，I组和II组受到40分钟的单侧肾脏缺血。 II组在缺血前接受1.5％的异氟烷​​20分钟。测量了JNK，P38和ERK的活动。 JNK和ERK在II组中的活性明显低于I组中的活动（p <0.05）。总之，在缺血之前给药时，异氟烷显示出针对肾脏缺血/再灌注损伤的预处理作用。抑制蛋白激酶JNK和ERK可能参与了机制。

项目成果

Masanori Matsumoto, Hiroaki Morooka, et al.: "Isoflurane inhibits NFkB activation in response to simulated ischemia in renal tubular cell line, LLC-PK1"Anesthesiology. 99. A1545 (2003)

Masanori Matsumoto、Hiroaki Morooka 等人：“异氟烷抑制 NFkB 激活以响应肾小管细胞系 LLC-PK1 中的模拟缺血”麻醉学。

N-acetyl-L-cysteine inhibits activitation of p38 MAP kinase in heat stressed kidney.

N-乙酰基-L-半胱氨酸抑制热应激肾脏中 p38 MAP 激酶的激活。

• 发表时间: 2002
• 期刊: Anesthesiology 97
• 作者: Hiroshi Miyoshi;Hiroaki Morooka;et al.
• 通讯作者: et al.

Hideo Hashiguchi, Hiroaki Morooka, et al.: "Pharmacological preconditioning effect of isoflurane against renal ischemia/reperfusion injury in rats"Anesthesiology. 99. A83 (2003)

Hideo Hashiguchi、Hiroaki Morooka 等：“异氟烷对大鼠肾缺血/再灌注损伤的药理预处理作用”麻醉学。

Hiroshi Miyoshi, Hiroaki Morooka, Toshiya Tsujita, et al.: "The protective effect of isoflurane against renal ischemia/reperfusion injury in rats"Anesthesiology. 97. A-78 (2002)

Hiroshi Miyoshi、Hiroaki Morooka、Toshiya Tsujita 等：“异氟烷对大鼠肾缺血/再灌注损伤的保护作用”麻醉学。

Hiroshi Miyoahi, Hiroaki Morooka, Toshiya Tsujita, et al.: "The protective effect of isoflurane against renal ischemia/reperfusion injury"Anesthesiology. 97. A78 (2002)

Hiroshi Miyoahi、Hiroaki Morooka、Toshiya Tsujita 等：“异氟烷对肾缺血/再灌注损伤的保护作用”麻醉学。