Pharmacological preconditioning effect of isoflurane against renal ischemia/reperfusion injury in rats.

异氟烷对大鼠肾缺血/再灌注损伤的药理预处理作用。

• 批准号: 14571448
• 负责人: MOROOKA Hiroaki
• 金额: $ 2.18万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571448/
• 关键词: SAPK; JNK; p38 MAPK; Ischemia-Reperfusion; ERK; isoflurane; 薬理学的プレコンディショニング; 麻酔薬; 腎機能障害

Isoflurane had a pharmacological preconditioning effect in the heart and brain. In this study, we investigated about the kidney and the mechanism. Rats were divided into four groups (n=8 for each). In groups A and B were sham surgery. Groups C and D were subjected to 40-min bilateral renal ischemia. Groups B and D received 1.5 % isoflurane for 20 min before sham surgery or ischemia. Blood sampling was performed at 12,24 and 48 hrs after reperfusion. The values of serum creatinine and blood urea nitrogen showed significant increases in group C compared to D at 24 and 48 hrs (p<0.01). Additionally in the histopathological examination, suppression of the damage was seen with the isoflurane treatment. To investigate the mechanism, group I and II were subjected to 40-min unilateral renal ischemia. Group II received 1.5 % isoflurane for 20 min before ischemia. The activities of JNK, p38 and ERK were measured. The activities of JNK and ERK in group II were significantly lower than those in group I (p<0.05). In conclusion, isoflurane shows preconditioning effect against renal ischemia/reperfusion injury when administered before ischemia. Inhibition of the protein kinases, JNK and ERK, might be involved in the mechanisms.

异氟醚对心脏和大脑具有药理预处理作用。在这项研究中，我们研究了肾脏及其机制。将大鼠分为四组（每组 n=8）。 A组和B组进行假手术。 C组和D组进行40分钟的双侧肾缺血。 B 组和 D 组在假手术或缺血前接受 1.5% 异氟烷 20 分钟。再灌注后12，24和48小时进行血液采样。 24小时和48小时时，C组的血清肌酐和血尿素氮值较D组显着升高（p<0.01）。此外，在组织病理学检查中，发现异氟烷治疗对损伤的抑制。为了研究其机制，I 组和 II 组接受 40 分钟的单侧肾缺血。第 II 组在缺血前接受 1.5% 异氟烷 20 分钟。测量JNK、p38和ERK的活性。 II组JNK和ERK活性显着低于I组(p<0.05)。总之，在缺血前给药时，异氟醚显示出针对肾缺血/再灌注损伤的预处理作用。蛋白激酶 JNK 和 ERK 的抑制可能与该机制有关。

项目成果

The protective effect of isoflurane against renal ischemia/reperfusion injury in rats

异氟烷对大鼠肾缺血/再灌注损伤的保护作用

• 发表时间: 2003
• 期刊: Anesthesiology 96
• 作者: Yoshitomi O;Hara T;Akiyama D;Oshibuchi M;Sumikawa K;Hiroshi Miyoshi
• 通讯作者: Hiroshi Miyoshi

Ropivacaine inhibits neurite outgrowth in PC-12 cells

• DOI: 10.1213/01.ane.0000130650.47552.18
• 发表时间: 2004-09-01
• 期刊: ANESTHESIA AND ANALGESIA
• 影响因子: 5.7
• 作者: Todoroki, S;Morooka, H;Sumikawa, K
• 通讯作者: Sumikawa, K

Isoflurane inhibits NFkB activation in response to simulated ischemia in renal tubular cell line, LLC-PK1

异氟烷抑制肾小管细胞系 LLC-PK1 模拟缺血时 NFkB 的激活

• 发表时间: 2003
• 期刊: Anesthesiology 99
• 作者: Yoshitomi O;Hara T;Akiyama D;Oshibuchi M;Sumikawa K;Hiroshi Miyoshi;Hiroaki Murata;Sungsam Cho;Guao Huan;Sungsam Cho;Masanori Matsumoto
• 通讯作者: Masanori Matsumoto

Masanori Matsumoto, Hiroaki Morooka, et al.: "Isoflurane inhibits NFkB activation in response to simulated ischemia in renal tubular cell line, LLC-PK1"Anesthesiology. 99. A1545 (2003)

Masanori Matsumoto、Hiroaki Morooka 等人：“异氟烷抑制 NFkB 激活以响应肾小管细胞系 LLC-PK1 中的模拟缺血”麻醉学。

N-acetyl-L-cysteine inhibits activitation of p38 MAP kinase in heat stressed kidney.

N-乙酰基-L-半胱氨酸抑制热应激肾脏中 p38 MAP 激酶的激活。

• 发表时间: 2002
• 期刊: Anesthesiology 97
• 作者: Hiroshi Miyoshi;Hiroaki Morooka;et al.
• 通讯作者: et al.