Research for ischemic tolerance in the spinal cord induced by the electrical convulsion therapy.

电惊厥治疗引起的脊髓缺血耐受性的研究。

• 批准号: 14571454
• 负责人: KAKINOHANA Manabu
• 金额: $ 2.24万
• 依托单位: University of the Ryukyus
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571454/
• 关键词: Spinal cord ischemia; Electrical convulsion therapy; Spinal blood flow; Ishcemic Tolerance; Paraplegia; ラット; 電気痙攣療法; c-fos; HSP

This study examines whether electrical stimuli on the spinal cord is also capable of inducing ischemic tolerance to ischemic spinal cord injury induced by transient aortic occlusion. The pretreatment of epidural electrical-stimulation(5mA,10s) was administered via the epidural silver electrodes. Spinal cord ischemia (SCI) was induced by the placement and subsequent inflation of a 2F Fogarty catheter which was inserted the descending thoracic aorta and combined with systemic hypotension(40mmHg). Animals were divided into four groups employed as follows ; Rapid Preconditioning-rats implanted with the epidural electrodes were exposed 9min of aortic occlusion 30min after the sham pretreatment of epidural electrical-stimulation (group RC:n=8) or the pretreatment of epidural electrical-stimulation (group RE:n=8) under isoflurane anesthesia, Delayed Preconditioning-rats implanted with the epidural electrodes were exposed 9min of aortic occlusion,24 hours after the sham pretreatment of epidural electrical-stimulation (group DC:n=8) or the pretreatment of epidural electrical-stimulation(group DE:n=8) under isoflurane anesthesia. In addition, rats were exposed with 6 up to l11min of SCI at 24 hours after epidural electrical-stimulation or sham stimulation. The group P50 represents the duration of SCI associated with 50% probability of resultant paraplegia.Our results showed that pretreatment of electrical stimulation at 24h, but not 30min before ischemia, protect the spinal cord against ischemic insults by aortic occlusion and that this stimulation prolong P50 by a approximately 15.0% compared with the control group. Since there is no difference in the spinal cord blood flow, measured by laser flow meter, during aortic occlusion between the group DE and DC, the neuroprotective effect in the group DE is not caused by the increase of collateral flow in spinal cord.

本研究探讨脊髓上的电刺激是否也能够诱导对短暂主动脉闭塞引起的缺血性脊髓损伤的缺血耐受。通过硬膜外银电极进行硬膜外电刺激（5mA，10s）的预处理。 2F Fogarty 导管插入胸降主动脉并随后充气并伴有全身性低血压（40mmHg），诱发脊髓缺血（SCI）。将动物分为如下四组；快速预处理——植入硬膜外电极的大鼠暴露主动脉闭塞9min，假手术预处理硬膜外电刺激（RC组：n=8）或异氟醚麻醉下硬膜外电刺激预处理（RE：n=8组）后30min；延迟预处理——植入硬膜外电极的大鼠暴露9min 异氟醚麻醉下硬膜外电刺激假手术预处理(DC组:n=8)或硬膜外电刺激预处理(DE组:n=8)后24小时主动脉闭塞。此外，在硬膜外电刺激或假刺激后24小时，将大鼠暴露于6至111分钟的SCI。 P50 组代表与导致截瘫的 50% 概率相关的 SCI 持续时间。我们的结果表明，在缺血前 24 小时而不是 30 分钟进行电刺激预处理，可以保护脊髓免受主动脉闭塞引起的缺血性损伤，并且与对照组相比，这种刺激使 P50 延长约 15.0%。由于激光流量计测得DE组和DC在主动脉封堵期间脊髓血流没有差异，因此DE组的神经保护作用不是由脊髓侧支血流增加引起的。

项目成果

S NAKAMURA, M KAKINOHANA, K SUGAHARA, S KINJO, Y MIYATA: "Intrathecal morphine, but not buprenorphine or pentazocine, can induce spastic paraparesis after noninjurious interval of spinal cord ischemia in the rats."Anesth Analg.. (in press). (2004)

S NAKAMURA、M KAKINOHANA、K SUGAHARA、S KINJO、Y MIYATA：“鞘内注射吗啡，但不是丁丙诺啡或喷他佐辛，可以在大鼠脊髓缺血的非损伤性间歇期后诱发痉挛性截瘫。”Anesth Analg..（出版中）。

S Nakamura, M Kakinohana, et al.: "Intrathecal Morphine, but not pentazocine or bupreorphine, can induce spastic paraparesis after noninjurious interval of spinal cord ischemia in the rats."Anesthesia and Analgesia. (in press). (2004)

S Nakamura、M Kakinohana 等人：“鞘内注射吗啡，但不是喷他佐辛或丁丙诺啡，可以在大鼠脊髓缺血的非损伤性间歇期后诱发痉挛性截瘫。” 麻醉和镇痛。

M KAKINOHANA, K SUGAHARA: "Anesthetic managements for preventing spinal cord injury in thoracoabdominal aneurysm repair surgery."Opioid inducing paraplegia-Cardiovasc Anesth. 8(1). 89-93 (2004)

M KAKINOHANA、K SUGAHARA：“胸腹动脉瘤修复手术中预防脊髓损伤的麻醉管理。”阿片类药物诱导截瘫 - 心血管麻醉。

垣花 学, 須加原 一博: "脊髄保護を念頭に入れた胸腹部大動脈瘤の周術期管理〜オピオイドと虚血性脊髄障害との関係〜"Cardiovascular Anesthesia. 8. 89-93 (2004)

Manabu Kakihana、Kazuhiro Sukahara：“考虑脊髓保护的胸腹主动脉瘤围手术期管理 - 阿片类药物与缺血性脊髓损伤之间的关系”心血管麻醉。

S Nakamura, M Kakinohana, et al.: "Intrathecal Morphine, but not pentazocine or buprenorphine, can induce spastic paraparesis after noninjurious interval of spinal cord ischemia in the rats"Anesthesia and Analgesia. (in Press). (2004)

S Nakamura、M Kakinohana 等人：“鞘内注射吗啡，但不是喷他佐辛或丁丙诺啡，可以在大鼠脊髓缺血的非损伤性间歇期后诱发痉挛性截瘫”麻醉和镇痛。