Effect of inhalational anesthetics for HIF-α mRNA expression in the Septic shock

吸入麻醉药对感染性休克中 HIF-α mRNA 表达的影响

• 批准号: 14571472
• 负责人: ONODERA Hidetaka
• 金额: $ 2.18万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571472/
• 关键词: c-fos; Propofol; midline thalamic nuculei; in situ hybridization; HIF; 低酸素; LPS; エンドトキシンショック; 出血性ショック

We investigate intravenous anesthetic Propofol (2,6-di-isopropylp h enol) suppress the induction of c-fos mRNA in the rat neocortex which expression begin to increase 2 hours before the dark period and remained elevated following the dark period in a normal rat.Simultaneously, administration of Propofol induce the expression of c-fos mRNA in the midline thalamic nuclei which is also activated by some benzodiazepine narcotic drugs. These indicate that narcotic action of Propofol exhibit via activation of midline thalamic nuclei. We also investigate that c-fos mRNA expression in the olivary nucleus in the medulla and in cereberal cortex in administration of Propofol in the rate of 20mg/kg and 30mg/kg. Rate of 10mg/kg do not induce the c-fos mRNA in these nucleus and cortex suggest that comparative high dose administration of propofol have some affects for these nucleus.

我们研究了静脉麻醉异丙酚（2,6-二异丙基苯酚）对大鼠新皮层c-fos mRNA表达的抑制作用，其表达在黑暗期前2小时开始增加，在正常大鼠黑暗期后保持升高。同时，异丙酚可诱导丘脑中线核c-fos mRNA的表达，该mRNA也可被苯二氮类药物激活。这表明异丙酚的麻醉作用是通过激活丘脑中线核来表现的。我们还研究了异丙酚在20mg/kg和30mg/kg剂量下，脑髓橄榄核和大脑皮层中c-fos mRNA的表达。10mg/kg剂量的异丙酚对大鼠核和皮层的c-fos mRNA无诱导作用，提示相对高剂量异丙酚对这些核有一定影响。

项目成果

Hidetaka Onodera et al.: "Differential expression of c-fos mRNA in the rat neocortex by in Situ hybridization"Life Science. 64(13). 1127-1135 (1999)

Hidetaka Onodera 等人：“通过原位杂交研究大鼠新皮质中 c-fos mRNA 的差异表达”生命科学。

Imaki J, Onodera H. et al.: "Developmental expression of mafB messenger ribonucleic acids in the rat skin by in situ hybridization imnohistochemistry"Journal of Delmatological Science. 33(2). 134-136 (2003)

Imaki J、Onodera H. 等人：“通过原位杂交免疫组织化学研究大鼠皮肤中 mafB 信使核糖核酸的发育表达”皮肤病学杂志。

Imaki J, Onodera H.et al.: "Developmental expression of mafB messenger ribonucleic acids in the rat skin by in situ hybridization imnohistochemistry"Journal of Delmatological Science. 33(2). 134-136 (2003)

Imaki J、Onodera H.等人：“通过原位杂交免疫组织化学研究大鼠皮肤中 mafB 信使核糖核酸的发育表达”皮肤病学杂志。