Morphological analysis of growth factors in the prostate by means if IFG-1/FGF-2 transgenic mice.

通过 IFG-1/FGF-2 转基因小鼠对前列腺生长因子进行形态学分析。

• 批准号: 14571488
• 负责人: TAKEUCHI Takumi
• 金额: $ 1.86万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571488/
• 关键词: Prostate; Growth Factor; transgenic; 前立腺癌; dermatopontin

Growth factors such as IGF-1/FGF-2 are involved in the carcinogenesis and growth in the prostate via autocrine/paracrine mechanism. We have established trasgenic mice expressing IGF-1/FGF-2 in the prostate epithelium under the control of probasin promotor and analysed histological changes in each prostatic lobe. Morphological analysis of IFG-1 and FGF2 transgenic prostate was performed for Fl and FO mice, respectively. Each lobal area was measured by computer-assisted morphometer. IGF1 transgenic mice expressed IGF-l protein in the ventral, dorsal, and lateral lobar epithelium and expressed IGFBP-3 in the epithelium of ventral lobe. Galndular and luminal sizes of those lobes were enlarged while epithelial growth was not observed. FGF-2 transgenic mice expressed FGF-2 and FGFR-2 proteins in the ventral, dorsal, and lateral lobes. Epithelial hyperplasia was seen in the dorsal lobe and Glandular and luminal enlargement without epithelial growth were observed in the ventral lobe. No morphological changes compared with controls were seen in the lateral and anterior lobes. Differential morphological changes in the prostate were introduced by different growth factors.

生长因子如IGF-1/FGF-2通过自分泌/旁分泌机制参与前列腺癌的发生和生长。我们建立了在probasin启动子控制下在前列腺上皮中表达IGF-1/FGF-2的转基因小鼠，并分析了每个前列腺叶的组织学变化。分别对F1和FO小鼠进行IFG-1和FGF 2转基因前列腺的形态学分析。用计算机辅助的形态计测量每个突起的面积。IGF 1转基因小鼠在腹叶、背叶和侧叶上皮表达IGF-I蛋白，在腹叶上皮表达IGFBP-3。这些叶的腺体和管腔尺寸增大，但未观察到上皮生长。FGF-2转基因小鼠在腹叶、背叶和侧叶中表达FGF-2和FGFR-2蛋白。在背叶中观察到上皮增生，在腹叶中观察到腺体和管腔增大，但无上皮生长。与对照组相比，在外侧叶和前叶中未观察到形态学变化。前列腺的不同形态学变化是由不同的生长因子引起的。

项目成果

Konno-Takahashi N, Takeuchi T et al.: "Engineered IGF-I expression induces glandular enlargement in the murine prostate."Journal of Endocrinology. 177. 389-398 (2003)

Konno-Takahashi N、Takeuchi T 等人：“工程化 IGF-I 表达诱导小鼠前列腺腺体增大。”内分泌学杂志。

Konno-Takahashi N, Takeuchi T et al.: "Engineed FGF-2 expression induces glandular epithelial hyperplasia in the murine prostatic dorsal lobe."European Urology. (in press).

Konno-Takahashi N、Takeuchi T 等人：“工程化 FGF-2 表达诱导小鼠前列腺背叶腺上皮增生。”欧洲泌尿学。

Konno-Takahashi N, Takeuchi T et al.: "Engineered IGF-1 expression induces glandular enlargement in the murine prostate."Journal of Endocrinology. 389-398 (2003)

Konno-Takahashi N、Takeuchi T 等人：“工程化 IGF-1 表达诱导小鼠前列腺腺体增大。”内分泌学杂志。

Konno-Takahashi N, Takeuchi T et al.: "Engineered IFG-1 expression induces glandular enlargement in the murine prostate."Journal of endocrinology. 177. 389-398 (2003)

Konno-Takahashi N、Takeuchi T 等人：“工程化 IFG-1 表达诱导小鼠前列腺腺体增大。”内分泌学杂志。

Konno-Takahashi N, Takeuchi T et al.: "Engineered FGF-2 Expression Induces Glandular Epithelial Hyperplasia in the Murine Prostatic Dorsal Lobe."European Urology. (In press).

Konno-Takahashi N、Takeuchi T 等人：“工程化 FGF-2 表达诱导小鼠前列腺背叶腺上皮增生。”欧洲泌尿学。