Morphological analysis of growth factors in the prostate by means if IFG-1/FGF-2 transgenic mice.

生长因子如IGF-1/FGF-2通过自分泌/旁分泌机制参与前列腺癌的发生和生长。我们建立了在前列腺前启动子控制下表达IGF-1/FGF-2的转基因小鼠，并分析了前列腺各叶的组织学变化。分别对Fl和FO小鼠进行IFG-1和FGF2转基因前列腺的形态学分析。通过计算机辅助形态仪测量每个局部区域。IGF1转基因小鼠在腹、背、外侧叶上皮表达igf - 1蛋白，在腹叶上皮表达IGFBP-3蛋白。腺体和管腔大小增大，上皮细胞未见生长。FGF-2转基因小鼠在腹、背和侧叶表达FGF-2和FGFR-2蛋白。背叶上皮增生，腹叶腺体和管腔增大，未见上皮增生。与对照组相比，侧叶和前叶未见形态学改变。不同的生长因子引起不同的前列腺形态变化。

Growth factors such as IGF-1/FGF-2 are involved in the carcinogenesis and growth in the prostate via autocrine/paracrine mechanism. We have established trasgenic mice expressing IGF-1/FGF-2 in the prostate epithelium under the control of probasin promotor and analysed histological changes in each prostatic lobe. Morphological analysis of IFG-1 and FGF2 transgenic prostate was performed for Fl and FO mice, respectively. Each lobal area was measured by computer-assisted morphometer. IGF1 transgenic mice expressed IGF-l protein in the ventral, dorsal, and lateral lobar epithelium and expressed IGFBP-3 in the epithelium of ventral lobe. Galndular and luminal sizes of those lobes were enlarged while epithelial growth was not observed. FGF-2 transgenic mice expressed FGF-2 and FGFR-2 proteins in the ventral, dorsal, and lateral lobes. Epithelial hyperplasia was seen in the dorsal lobe and Glandular and luminal enlargement without epithelial growth were observed in the ventral lobe. No morphological changes compared with controls were seen in the lateral and anterior lobes. Differential morphological changes in the prostate were introduced by different growth factors.