The role of bone cytokine, CBFa-1, OPG and ODF in the mechanism of bone metastases with prostate cancers

骨细胞因子、CBFa-1、OPG、ODF在前列腺癌骨转移机制中的作用

• 批准号: 14571493
• 负责人: TAKIHANA Yoshio
• 金额: $ 2.24万
• 依托单位: University of Yamanashi (2003)山梨医科大学 (2002)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571493/
• 关键词: Bone metastases; Real-time PCR; Bone cytokine; ODF; OPG; CBFa-1; 骨転移; 骨関連蛋白

The mechanism for the osteoplastic metastases in prostate cancer is not clearly known but osteoplastic metastases might be correlated with the balance of osteoblast and osteoclast cell. The regulator factors of osteoblast and osteoclast cells (bone cytokine, OPG, ODF and CBFa-1) mignt have an important role in bone metastases. We evaluated the quantifcation of gene expression of bone regulator factors in the cancer cells. We removed only the cancer cells from the specimens of prostate cancer, bladder cancer and renal cell cancer using laser-captured microdissection. We measured the real-time RT-PCR quantification of BMP-2, BMP-6, CBFa-1, PHT-rP, ODF and OPG. The relative values of mRNA were presented as each factors/β-actin ×1000. The relative values of BMP-2, BMP-6, OPG, CBFa-1, PTH-rP and ODF ware 14.33±3.30,21.18±1.35,4.71±1.29,3.47±1.62,24.45±8.10 and 1.29±0.65 in prostate cancer, respectively. The BMP-2, BMP-6, OPG, CBFa-1, PTH-rP and ODF were 0.95±0.20,1.30±0.60,1.52±0.36,0.55±0.36,0.29±0.13 and 2.20±1.28 in renal cell cancer, respectively. The mRNA expression of BMP-2, BMP-6, OPG, CBFa-1, PTH-rP and ODF were 1.19±0.56,0.18±0.11,0.26±0.14,0.62±0.20,0.12±0.06 and 0.13±0.09 in bladder cancer, respectively. The relative OCIF, PTH-rP and BMP-2, BMP-6 values in prostate cancer were significantly higher than that in bladder cancer and renal cell cancer. The relative BMP-2, BMP-6 and PTH-rP values were significantly higher than other factors in prostate cancer. The regulator factors of bone formation might be an essential role in mechanism of bone metastases from prostate cancer. Elucidating the mechanism of bone metastasis will be useful to treat the advanced prostate cancer.

前列腺癌骨转移的机制尚不清楚，但骨转移可能与成骨细胞和破骨细胞的平衡有关。成骨细胞和破骨细胞的调节因子（骨细胞因子、OPG、ODF和CBFa-1）可能在骨转移中起重要作用。我们评估了骨调节因子在癌细胞中的基因表达的定量。我们使用激光捕获显微切割技术仅从前列腺癌、膀胱癌和肾细胞癌标本中去除癌细胞。我们测量了BMP-2、BMP-6、CBFa-1、PHT-rP、ODF和OPG的实时RT-PCR定量。mRNA的相对值表示为每个因子/β-actin ×1000。前列腺癌中BMP-2、BMP-6、OPG、CBFa-1、PTH-rP和ODF的相对值分别为14.33± 3.30、21.18 ± 1.35、4.71 ± 1.29、3.47 ± 1.62、24.45 ±8.10和1.29±0.65。肾细胞癌中BMP-2、BMP-6、OPG、CBFa-1、PTH-rP和ODF的表达分别为0.95± 0.20、1.30 ± 0.60、1.52 ± 0.36、0.55 ± 0.36、0.29 ±0.13和2.20±1.28。BMP-2、BMP-6、OPG、CBFa-1、PTH-rP和ODF的mRNA表达分别为1.19± 0.56、0.18 ± 0.11、0.26 ± 0.14、0.62 ± 0.20、0.12 ±0.06和0.13±0.09。前列腺癌组织中OCIF、PTH-rP和BMP-2、BMP-6的相对值均显著高于膀胱癌和肾癌组织。BMP-2、BMP-6和PTH-rP的相对值在前列腺癌中显著高于其他因子。骨形成的调节因子可能在前列腺癌骨转移的发生机制中起重要作用。阐明前列腺癌骨转移的发生机制，将有助于晚期前列腺癌的治疗。