ShEEP Request for QuantStudio 12K Flex Real-Time PCR system

ShEEP 请求 QuantStudio 12K Flex 实时 PCR 系统

• 批准号: 9796289
• 负责人: PAULA C BICKFORD
• 金额: --
• 依托单位: JAMES A. HALEY VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2019-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9796289
• 关键词: Biological Assay; Caliber; Complementary DNA; Data; Data Collection; Equipment; Failure; Funding; Genes; Goals; Healthcare; Hour; Hydrophobicity; Investigation; Laboratories; Lead; Lightning; Liquid substance; Maintenance; Microfluidics; Microscope; Power Sources; Reaction; Reagent; Research; Research Personnel; Resources; Running; Sampling; Sheep; Slide; Surface Tension; System; Technology; Time; cost; flexibility; hydrophilicity; instrument; nanolitre; novel; operation; parallel processing; screening

Abstract We are requesting Fisher HealthCare QuantStudioTM 12K Flex Real-Time PCR System. This instrument can simultaneously run up to four 3,072-reaction QuantStudioTM 12K Flex OpenArray® Plates in about 4 hours. A streamlined workflow and automated plate handling minimize start-up and hands-on time. Thus, this system will allow maximum throughput with minimal resources. This system combines flexible throughput capabilities with a streamlined workflow, the Fisher HealthCare QuantStudio™ 12K Flex system allows for targeted discovery through confirmation and screening, all on a single platform. OpenArray® technology is a broadly applicable nanoliter fluidics platform for low-volume, solutionphase reactions, and enables lower reagent and assay costs, and rapid parallel processing. The OpenArray® technology uses a microscope slide–sized plate with 3,072 through-holes. Each plate contains 48 subarrays, each with 64 through-holes. Each through-hole is 300 μm in diameter and 300 μm deep and is treated with hydrophilic and hydrophobic coatings. Reagents are retained in the through-holes via surface tension. A major advantage is the small amount of sample needed for analysis. Only 2µg of cDNA per sample is needed to assay over 600 target genes due to the low volume fluidics platform. One OpenArray® microfluidics panel can hold as many samples as eight traditional 384-well plates. This machine also run traditional 384 well plates. This equipment will significantly enhance the quality of research for PI’s at the James A Haley VA.

抽象的 我们需要 Fisher HealthCare QuantStudioTM 12K Flex 实时 PCR 系统。这 仪器最多可同时运行四个 3,072 反应 QuantStudioTM 12K Flex OpenArray® 板大约需要 4 小时。简化的工作流程和自动化的板处理 最大限度地减少启动和动手时间。因此，该系统将允许最大吞吐量 最少的资源。该系统结合了灵活的吞吐量能力和简化的 工作流程中，Fisher HealthCare QuantStudio™ 12K Flex 系统可实现有针对性的 通过确认和筛选进行发现，所有这些都在一个平台上进行。 OpenArray® 技术是一种广泛适用的纳升流体平台，适用于低容量、溶液相 反应，并能够降低试剂和测定成本，以及快速并行处理。这 OpenArray® 技术使用显微镜载玻片大小的板，具有 3,072 个通孔。每个 该板包含 48 个子阵列，每个子阵列有 64 个通孔。每个通孔的尺寸为 300 μm 直径300微米深，并经过亲水和疏水涂层处理。 试剂通过表面张力保留在通孔中。一个主要优点是 分析需要少量样品。每个样品仅需要 2 µg cDNA 由于流体平台体积小，可检测 600 多个目标基因。一个 OpenArray® 微流控面板可容纳与八个传统 384 孔板一样多的样品。这 机器还运行传统的 384 孔板。该设备将显着提高 James A Haley VA 的 PI 的研究质量。