ShEEP Request for QuantStudio 12K Flex Real-Time PCR system

ShEEP 请求 QuantStudio 12K Flex 实时 PCR 系统

• 批准号: 9796289
• 负责人: PAULA C BICKFORD
• 金额: --
• 依托单位: JAMES A. HALEY VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2019-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9796289
• 关键词: Biological Assay; Caliber; Complementary DNA; Data; Data Collection; Equipment; Failure; Funding; Genes; Goals; Healthcare; Hour; Hydrophobicity; Investigation; Laboratories; Lead; Lightning; Liquid substance; Maintenance; Microfluidics; Microscope; Power Sources; Reaction; Reagent; Research; Research Personnel; Resources; Running; Sampling; Sheep; Slide; Surface Tension; System; Technology; Time; cost; flexibility; hydrophilicity; instrument; nanolitre; novel; operation; parallel processing; screening

Abstract We are requesting Fisher HealthCare QuantStudioTM 12K Flex Real-Time PCR System. This instrument can simultaneously run up to four 3,072-reaction QuantStudioTM 12K Flex OpenArray® Plates in about 4 hours. A streamlined workflow and automated plate handling minimize start-up and hands-on time. Thus, this system will allow maximum throughput with minimal resources. This system combines flexible throughput capabilities with a streamlined workflow, the Fisher HealthCare QuantStudio™ 12K Flex system allows for targeted discovery through confirmation and screening, all on a single platform. OpenArray® technology is a broadly applicable nanoliter fluidics platform for low-volume, solutionphase reactions, and enables lower reagent and assay costs, and rapid parallel processing. The OpenArray® technology uses a microscope slide–sized plate with 3,072 through-holes. Each plate contains 48 subarrays, each with 64 through-holes. Each through-hole is 300 μm in diameter and 300 μm deep and is treated with hydrophilic and hydrophobic coatings. Reagents are retained in the through-holes via surface tension. A major advantage is the small amount of sample needed for analysis. Only 2µg of cDNA per sample is needed to assay over 600 target genes due to the low volume fluidics platform. One OpenArray® microfluidics panel can hold as many samples as eight traditional 384-well plates. This machine also run traditional 384 well plates. This equipment will significantly enhance the quality of research for PI’s at the James A Haley VA.

摘要 我们请求Fisher HealthCare QuantStudioTM 12 K Flex实时PCR系统。这 仪器可同时运行多达4个3，072反应QuantStudioTM 12 K Flex OpenArray®板在约4小时内。简化的工作流程和自动化平板处理 最大限度地减少启动和动手时间。因此，该系统将允许最大吞吐量， 最少的资源。该系统将灵活的吞吐能力与流线型的 Fisher HealthCare QuantStudio™ 12 K Flex系统允许有针对性地 通过确认和筛选进行发现，所有这些都在一个平台上进行。OpenArray® 技术是一种广泛适用于低体积、溶液相 反应，并且能够降低试剂和测定成本，以及快速并行处理。的 OpenArray®技术使用显微镜载玻片大小的板，具有3，072个通孔。每个 板包含48个子阵列，每个子阵列有64个通孔。每个通孔为300 μm in 直径为300 μm，并经过亲水和疏水涂层处理。 试剂通过表面张力保留在通孔中。一个主要的优点是 分析所需样品量少。每个样品仅需要2µg cDNA 由于低容量射流平台，可测定超过600个靶基因。一个OpenArray 微流体板可以容纳与8个传统384孔板一样多的样品。这 机器也运行传统的384孔板。该设备将大大提高 詹姆斯·A·哈利弗吉尼亚州PI的研究质量。