ShEEP Request for QuantStudio 12K Flex Real-Time PCR system

ShEEP 请求 QuantStudio 12K Flex 实时 PCR 系统

• 批准号: 9796289
• 负责人: PAULA C BICKFORD
• 金额: --
• 依托单位: JAMES A. HALEY VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2019-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9796289
• 关键词: Biological Assay; Caliber; Complementary DNA; Data; Data Collection; Equipment; Failure; Funding; Genes; Goals; Healthcare; Hour; Hydrophobicity; Investigation; Laboratories; Lead; Lightning; Liquid substance; Maintenance; Microfluidics; Microscope; Power Sources; Reaction; Reagent; Research; Research Personnel; Resources; Running; Sampling; Sheep; Slide; Surface Tension; System; Technology; Time; cost; flexibility; hydrophilicity; instrument; nanolitre; novel; operation; parallel processing; screening

Abstract We are requesting Fisher HealthCare QuantStudioTM 12K Flex Real-Time PCR System. This instrument can simultaneously run up to four 3,072-reaction QuantStudioTM 12K Flex OpenArray® Plates in about 4 hours. A streamlined workflow and automated plate handling minimize start-up and hands-on time. Thus, this system will allow maximum throughput with minimal resources. This system combines flexible throughput capabilities with a streamlined workflow, the Fisher HealthCare QuantStudio™ 12K Flex system allows for targeted discovery through confirmation and screening, all on a single platform. OpenArray® technology is a broadly applicable nanoliter fluidics platform for low-volume, solutionphase reactions, and enables lower reagent and assay costs, and rapid parallel processing. The OpenArray® technology uses a microscope slide–sized plate with 3,072 through-holes. Each plate contains 48 subarrays, each with 64 through-holes. Each through-hole is 300 μm in diameter and 300 μm deep and is treated with hydrophilic and hydrophobic coatings. Reagents are retained in the through-holes via surface tension. A major advantage is the small amount of sample needed for analysis. Only 2µg of cDNA per sample is needed to assay over 600 target genes due to the low volume fluidics platform. One OpenArray® microfluidics panel can hold as many samples as eight traditional 384-well plates. This machine also run traditional 384 well plates. This equipment will significantly enhance the quality of research for PI’s at the James A Haley VA.

摘要 我们需要Fisher Healthcare QuantStudioTM 12K Flex实时聚合酶链式反应系统。这 仪器最多可同时运行4台3,072-Reaction QuantStudioTM 12K Flex Open阵列®制版约需4小时。简化的工作流程和自动化的板材处理 最大限度地减少启动时间和实际操作时间。因此，该系统将允许最大吞吐量 最少的资源。该系统结合了灵活的吞吐能力和简化的 工作流程，费舍尔医疗保健QuantStudio™12K灵活系统允许有针对性 通过确认和筛选进行发现，所有这些都在单一平台上进行。OpenArray® 技术是一种广泛适用的纳升流体平台，适用于低体积、溶液相 反应，并实现更低的试剂和检测成本，以及快速并行处理。这个 OpenArray®技术使用带有3,072个通孔的显微镜载玻片大小的平板。每个人 盘片包含48个子阵，每个子阵上都有通孔。每个通孔为300μm英寸 直径和深度为300μm，采用亲水和疏水涂层处理。 试剂通过表面张力保留在通孔中。一个主要的优势是 分析所需的少量样品。每个样本只需要2微克的cDNA 由于低流量的流体平台，可以分析600多个靶基因。One OpenArray® 微流体面板可以容纳多达8个传统的384孔板的样品。这 机器还运行传统的384井板。该设备将显著增强 詹姆斯·A·海利退伍军人学院私家侦探的研究质量。