Regulation of Growth and Differentiation of Human Urethral Rhabdosphincter Cells

人尿道横纹括约肌细胞生长和分化的调控

• 批准号: 14571508
• 负责人: MIMATA Hiromitsu
• 金额: $ 2.24万
• 依托单位: Oita University (2004)大分医科大学 (2002-2003)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571508/
• 关键词: stress incontinence; urethral rhabdosphincter; skeletal muscle; growth factor; 尿失禁; シグナル伝達; HGF; IGF-I; cDNA microarray; bFGF; サイトカイン; ストレス応答; MAPキナーゼ; PI3キナーゼ

Regeneration of urethral rhabdosphincter muscle will be a new possible therapy for urinary stress incontinence. We selectively cultured skeletal satellite cells from human urethral rhabdosphincter using neural cell adhesion molecule as a cell surface marker. Proliferation of satellite cells of urethral rhabdosphincter is enhanced by growth factors such as hepatocyte growth factor(HGF) and insulin-like growth factor-I(IGF-I) via MAPK and PI3-K signaling pathways, respectively. As the satellite cells produced both HGF and IGF-I and the neutralizing antibodies against HGF and IGF-I inhibited the growth, these growth factors stimulate the growth of the urethral rhabdosphincter cells as autocrine frashion. These findings suggest that local injections of HGF and IGF-I around membranous urethra may be useful to regenerate urethral rhabdosphincter to treat stress incontinence.

尿道色刺肌的再生将是尿应激尿失禁的新疗法。我们使用神经细胞粘附分子作为细胞表面标记有选择性地培养的骨骼卫星细胞从人类尿道纹状体中。通过MAPK和PI3-K信号通路，分别通过肝细胞生长因子（HGF）（HGF）和胰岛素样生长因子I（IGF-I）等生长因子（例如肝细胞生长因子（HGF）和胰岛素样生长因子I（IGF-I））增强了尿道色丁蛋白的卫星细胞的增殖。由于卫星细胞同时产生了HGF和IGF-I，并且针对HGF和IGF-I的中和抗体抑制了生长，因此这些生长因子刺激了作为自分泌剂的尿道胸腺磷酸细胞的生长。这些发现表明，HGF和IGF-I围绕膜尿道的局部注射可能有助于再生尿道纹状杆菌以治疗应激尿失禁。

项目成果

腹圧性尿失禁に対する近未来の治療戦略

压力性尿失禁的未来治疗策略

• 发表时间: 2005
• 期刊: 西日本泌尿器科 67・4
• 作者: Sumino Y;et al.;Y.Sumino;三股 浩光
• 通讯作者: 三股 浩光