The study of roles of Rho family GTPase in progression and metastasis of renal cell cancer

我们分析了RhoA，RhoB，RhoC和ROCK基因的mRNA水平的手术标本RCC组织从78个连续的日本患者和相应的非肿瘤组织来自同一患者使用逆转录后聚合酶链反应（RT-PCR）。结果表明，RhoC和ROCK在睾丸肿瘤组织中的表达水平明显高于非肿瘤组织（P<0.0001，P<0.0001）; RhoA的mRNA表达水平之间没有差异。RhoB mRNA在肿瘤组织中的表达水平明显高于非肿瘤组织（P<0.05），但其表达与肿瘤的分级、分期及预后无关。RhoC和ROCK mRNA表达水平与肿瘤分级有关（P<0.05，P<0.05）; ...更多信息 年龄（P<0.0001，P<0.0001）。RhoC和ROCK mRNA在肿瘤组织中的表达水平呈正相关（P<0.0001）。RhoC和ROCK mRNA水平低与高的患者生存率的Kaplan-Meier曲线显示，RhoC和ROCK mRNA水平高与总生存率相关（分别为P<0.0001，P<0.0001）。多因素分析显示RhoC和ROCK mRNA水平是影响总生存率的独立预后因素，在肾盂输尿管癌中，RhoC和ROCK蛋白的高表达与低分化分级、高分期和预后不良有关：1）在膀胱癌中，RhoC和ROCK蛋白的高表达与低分化分级、高分期、高分化程度和高分化程度有关，在睾丸生殖细胞肿瘤中，RhoA、ROCK、Rac 1和Cdc 42的高表达与疾病的晚期有关。少

We analyzed the mRNA levels of RhoA, RhoB, RhoC and ROCK gene in the surgical specimens of RCC tissues from 78 consecutive Japanese patients and in the corresponding non-tumor tissue originating from the same patient using polymerase chain reaction after reverse transcription (RT-PCR). We compared their expression levels in tumor tissues with those in non-tumor tissues and evaluated the relationship between their expression levels in tumors and clinicopathological features.The mRNA levels of RhoC and ROCK were increased in tumor tissues, compared with those in non-tumor tissues of the resected testis (P<0.0001, P<0.0001, respectively). There was no difference between the expression levels of mRNAs for RhoA. Although RhoB mRNA levels were higher in tumor tissues than those in non-tumor tissues (P<0.05), its expression was not associated with tumor grade, stage and prognosis. The increase of the RhoC and ROCK mRNA levels was related to the tumor grade (P<0.05, P<0.05, respectively) and s … More tage (P<0.0001, P<0.0001, respectively). There was a positive relationship between expression levels of mRNAs of RhoC and ROCK in tumor tissues (P<0.0001). Kaplan-Meier plots of survival rate in patients with low versus high mRNA levels of RhoC and ROCK showed that high mRNA levels of RhoC and ROCK were associated with overall survival (P<0.0001, P<0.0001, respectively). Multivariate analysis showed that mRNA levels of RhoC and ROCK were independent prognostic factors in overall survival.In renal pelvis and ureter cancer, the higher expression of RhoA and ROCK proteins were associated poorly differentiated grade, higher stage, and unfavorable prognosis 1).In bladder cancer, although the higher protein levels of RhoA, RhoC, and ROCK were related to poorly differentiated grade, higher stage, and unfavorable prognosis, the lower RhoB levels were shown in advanced tumors 2).In testicular germ cell tumor, the higher expressions of RhoA, ROCK, Rac1 and Cdc42 were associated with advanced stage of the disease 3). Less