Molecular investigation on prognostic factors and development of an tailor-made therapy for Hunt syndrome.

亨特综合征预后因素的分子研究和定制疗法的开发。

• 批准号: 14571602
• 负责人: FURUTA Yasushi
• 金额: $ 2.56万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571602/
• 关键词: Varicella-zoster virus; VZV DNA; Real-time PCR; Anti-VZV antibody; Ramsay Hunt syndrome; Bell's palsy; Zoster sine herpete; 水痘帯状庖疹ウイルス; VAV DNA量

1.Varicella-zoster virus DNA level and facial paralysis in Ramsay Hunt syndromeWe have investigated whether the copy number of varicella-zoster virus (VZV) in saliva correlates with the clinical symptoms in patients with Ramsay Hunt syndrome (RHS). Patients with oropharyngeal zoster showed worse recovery of facial function than those without. It seems that the VZV DNA level in saliva reflects the kinetics of viral reactivation in the facial nerve as well as in the oropharyngeal epithelium in RHS patients.2.Patterns of varicella-zoster virus reactivation in Ramsay Hunt syndromeWe analyzed the relationship between VZV load and onset of facial paralysis in 42 RHS patients. The results indicate that facial paralysis in RHS can occur at various times between the early and the regression phase of VZV reactivation, suggesting that there are variable patterns of development of facial nerve dysfunction caused by VZV reactivation and the progression of neuritis.3.Serological diagnosis of varicel … More la-zoster virus reactivation in patients with acute peripheral facial paralysisWe focused on the determination of an anti-VZV IgG antibody liter in sero-diagnosis by using an enzyme immunoassay (EIA) and investigated the diagnostic criteria for VZV reactivation by EIA. It was considered appropriate to give a diagnosis of VZV reactivation for those cases exhibiting a double-fold increasd in the VZV IgG EIA antibody titer.4. Analysis of varicella-zoster virus load and cochleovestibular symptoms in patients with Ramsay Hunt syndromeThe present study analyzed the relationship between VZV load and the onset of the cochleovestibular symptoms. The results indicate that cochleovestibular symptoms can occur in various timing from the early phase to the regression phase of VZV reactivation, suggesting that there are various patterns in the development of the eighth cranial nerve dysfunction caused by VZV reactivation and progression of neuritis.5.Varicella-zoster virus reactivation is the major cause of acute peripheral facial paralysis m childrenThis study analyzed the causes of acute peripheral facial paralysis in children by serological assays and PCR using paired sera and saliva samples. The results indicate that VZV reactivation is the major cause of acute peripheral facial paralysis in children, especially in ages between 6 and 15. In contrast, our data suggest that HSV-1 reactivation is not the major pathogenesis of Bell's palsy in children.6.Analysis of patients with Bell's palsy who showed incomplete recoveryThis study analyzed the causes of clinically diagnosed Bell's palsy who showed incomplete recovery of facial movement. The results indicate that VZV or HSV-1 reactivation is the major cause of Bell's palsy with poor prognosis. Less

1.水痘-带状疱疹病毒DNA水平与Ramsay Hunt综合征（RHS）面瘫的关系我们研究了Ramsay Hunt综合征（RHS）患者唾液中水痘-带状疱疹病毒（VZV）的拷贝数与临床症状的关系。口咽部带状疱疹患者面部功能恢复较差。唾液中VZV DNA水平反映了RHS患者面神经和口咽上皮中病毒再激活的动力学。2. Ramsay Hunt综合征中水痘-带状疱疹病毒再激活的模式我们分析了42例RHS患者VZV载量与面瘫发病的关系。结果表明，RHS患者的面瘫可发生在VZV再激活的早期和消退期之间的不同时间，提示VZV再激活引起的面神经功能障碍的发展和神经炎的进展存在不同的模式。 ...更多信息 急性周围性面神经麻痹患者带状疱疹病毒（VZV）再激活的研究我们采用酶免疫分析法（EIA）测定血清学诊断中的抗VZVIgG抗体滴度，并探讨EIA对VZV再激活的诊断标准。对于VZV IgG EIA抗体滴度增加一倍的病例，认为诊断为VZV再激活是适当的。Ramsay Hunt综合征患者水痘-带状疱疹病毒载量与耳蜗前庭症状分析本研究分析了VZV载量与耳蜗前庭症状发作的关系。结果表明，从VZV再激活的早期阶段到消退阶段，耳蜗前庭症状可以在不同的时间发生，提示在VZV再激活和神经炎进展引起的第八脑神经功能障碍的发展中存在多种模式。带状疱疹病毒再激活是儿童急性周围性面瘫的主要原因，本文分析了儿童急性周围性面瘫的病因，使用配对血清和唾液样本通过血清学测定和PCR检测儿童瘫痪。结果表明，VZV再激活是儿童急性周围性面瘫的主要原因，特别是在6至15岁之间。相反，我们的数据表明HSV-1再激活不是儿童Bell麻痹的主要发病机制。6. Bell麻痹患者恢复不完全的分析本研究分析了临床诊断的Bell麻痹患者面部运动恢复不完全的原因。提示VZV或HSV-1再激活是Bell麻痹的主要原因，预后差。少

项目成果

古田 康 他: "耳鼻咽喉科領域の皮膚・粘膜疾患 耳性帯状疱疹"耳喉頭頸. 75巻. 517-522 (2003)

Yasushi Furuta 等：“带状疱疹，耳鼻喉科领域的皮肤和粘膜疾病”，《耳鼻喉科和颈部》75. 517-522 (2003)。

Furuta Y, et al.: "Varicella-zoster virus DNA level and facial paralysis in Ramsay Hunt syndrome"Ann Otol Phinol Laryngol. (in press).

Furuta Y 等人：“拉姆齐亨特综合征中的水痘带状疱疹病毒 DNA 水平和面瘫”Ann Otol Phinol Laryngol。

大谷 文雄 他: "Hunt症候群における第8脳神経症状とVZV再活性化動態"Facial Nerve Research. 23巻. 92-94 (2003)

Fumio Otani 等人：“亨特综合征中的第 8 脑神经症状和 VZV 重新激活动态”，面部神经研究，第 23 卷，92-94 (2003)。

Furuta Y, et al.: "Varicella-zoster virus DNA level and facial paralysis in Ramsay Hunt syndrome"Ann Otol Rhinol Laryngol. (印刷中).

Furuta Y 等人：“拉姆齐亨特综合征中的水痘带状疱疹病毒 DNA 水平和面瘫”Ann Otol Rhinol Laryngol（正在出版）。

Ohtani F.: "Analysis of VZV load in patients with Hunt syndrome"Facial Nerve Research. 22. 50-52 (2002)

Ohtani F.：“亨特综合征患者 VZV 负荷分析”面神经研究。