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The heat shock protein(HSP) was investigated as the pathogenesis of focal infection with tonsil by the SCID mouse model

通过SCID小鼠模型研究热休克蛋白（HSP）作为扁桃体局灶性感染的发病机制

基本信息

批准号：
14571629
负责人：
FUJIHARA Keiji
金额：
$ 1.98万
依托单位：
Wakayama Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

<Introduction>In this study, we investigated the heat shock protein(HSP) as the relationship between tonsils and pustulosis palmaris et plantaris(PPP). We engrafted human cells onto severe combined immunodifficiency(SCID) mice, together with the human skin.<Material, Method and Results>1.We got the tonsil, peripheral blood and normal skin of sole from PPP patient with permission. We transplanted tonsillar or peripheral lymphocytes together with the sole skin into SCID mice. We examined the anti-bacterial HSP antibody by ELISA. DnaK is bacterial HSP 70. GroEL is bacterial HSP65. HSP-60 is also bacterial HSP. We got high titer of anti-DnaK antibody, anti-GroEL antibody and anti-HSP60IgG antibody just in the mice transplanted tonsillar lymphocytes with auto-sole skin.2.The engrafted skins were immuno-stained. The infiltrated lymphocytes were mainly CD4+ and CD8+ T lymphocytes.3.We got the high titer of anti HSP60IgG antibody, injected the HSP60 recombinant to the mice transplanted the tonsil lymphocytes.<Discussion>We already reported that tonsillar lymphocytes directly infiltrated into human sole skin in human skin/SCID mouse chimera model. In this study, we found high titer of anti-HSP antibody in the same model. It seemed that the tonsillar lymphocytes had already sensitized by bacterial HSP in crypts of tonsil. The lymphocytes may react with the HSP in human sole skin. The tonsillar lymphocytes of PPP may react with human sole skin by cell-immunity and humoral-immunity. We emphasized that the HSP may be one of the pathogen of PPP. The anti-HSP antibody may be the help of pre-operation diagnosis of focal infection of tonsil with PPP.

<Introduction>本研究旨在探讨热休克蛋白（HSP）与扁桃体和掌跖脓疱病（PPP）的关系。我们将人类细胞与人类皮肤一起移植到严重联合免疫缺陷（SCID）小鼠身上。材料、方法和结果1.经许可取PPP患者扁桃体、外周血及正常足底皮肤。我们将扁桃体或外周血淋巴细胞与足底皮肤一起移植到SCID小鼠体内。ELISA法检测抗细菌HSP抗体。DnaK是细菌HSP 70。GroEL是细菌HSP 65。HSP-60也是细菌HSP。自体足底皮肤移植小鼠扁桃体淋巴细胞后，可获得高滴度的抗DnaK抗体、抗GroEL抗体和抗HSP 60 IgG抗体。3.获得高滴度的抗HSP 60 IgG抗体，将重组HSP 60注射到扁桃体淋巴细胞移植小鼠体内。<Discussion>我们已经报道了在人皮肤/SCID小鼠嵌合体模型中扁桃体淋巴细胞直接浸润到人足底皮肤。在本研究中，我们在同一模型中发现了高滴度的抗HSP抗体。提示扁桃体淋巴细胞已被细菌性HSP致敏。人足底皮肤中的淋巴细胞可与HSP发生反应。PPP扁桃体淋巴细胞可能通过细胞免疫和体液免疫与人足底皮肤发生反应。我们认为HSP可能是PPP的病原之一。结论：抗HSP抗体可作为PPP扁桃体局灶性感染的术前诊断依据。