The heat shock protein(HSP) was investigated as the pathogenesis of focal infection with tonsil by the SCID mouse model

在这项研究中，我们研究了热休克蛋白(HSP)在扁桃体和掌底脓疱病(PPP)之间的关系。我们将人细胞与人皮肤一起植入重度联合免疫缺陷(SCID)小鼠体内。材料、方法及结果1.经允许，取PPP患者的扁桃体、外周血及足底正常皮肤。我们将扁桃体或外周淋巴细胞与足底皮肤一起移植到SCID小鼠体内。用酶联免疫吸附试验检测抗细菌热休克蛋白抗体。DNAK是细菌热休克蛋白70。GroEL是细菌型HSP65。HSP-60也是细菌性HSP。仅在移植自体底皮肤的小鼠扁桃体淋巴细胞中即可获得高滴度的抗DNAK抗体、抗GroEL抗体和抗HSP60Ig G抗体。我们获得了高滴度的抗HSP60抗体，将HSP60重组蛋白注射到移植扁桃体淋巴细胞的小鼠体内。在人皮肤/SCID小鼠嵌合模型中，我们已经报道了扁桃体淋巴细胞直接渗透到人的足底皮肤。在本研究中，我们在同一模型中发现了高滴度的抗HSP抗体。扁桃体淋巴细胞似乎已被扁桃体隐窝内的细菌热休克蛋白致敏。人足底皮肤中的淋巴细胞可与热休克蛋白发生反应。PPP的扁桃体淋巴细胞可能通过细胞免疫和体液免疫与人足底皮肤发生反应。我们强调HSP可能是PPP的病原体之一。抗HSP抗体有助于扁桃体局灶性感染的术前诊断。

<Introduction>In this study, we investigated the heat shock protein(HSP) as the relationship between tonsils and pustulosis palmaris et plantaris(PPP). We engrafted human cells onto severe combined immunodifficiency(SCID) mice, together with the human skin.<Material, Method and Results>1.We got the tonsil, peripheral blood and normal skin of sole from PPP patient with permission. We transplanted tonsillar or peripheral lymphocytes together with the sole skin into SCID mice. We examined the anti-bacterial HSP antibody by ELISA. DnaK is bacterial HSP 70. GroEL is bacterial HSP65. HSP-60 is also bacterial HSP. We got high titer of anti-DnaK antibody, anti-GroEL antibody and anti-HSP60IgG antibody just in the mice transplanted tonsillar lymphocytes with auto-sole skin.2.The engrafted skins were immuno-stained. The infiltrated lymphocytes were mainly CD4+ and CD8+ T lymphocytes.3.We got the high titer of anti HSP60IgG antibody, injected the HSP60 recombinant to the mice transplanted the tonsil lymphocytes.<Discussion>We already reported that tonsillar lymphocytes directly infiltrated into human sole skin in human skin/SCID mouse chimera model. In this study, we found high titer of anti-HSP antibody in the same model. It seemed that the tonsillar lymphocytes had already sensitized by bacterial HSP in crypts of tonsil. The lymphocytes may react with the HSP in human sole skin. The tonsillar lymphocytes of PPP may react with human sole skin by cell-immunity and humoral-immunity. We emphasized that the HSP may be one of the pathogen of PPP. The anti-HSP antibody may be the help of pre-operation diagnosis of focal infection of tonsil with PPP.