Molecular immune mechanisum of VKH disease.

VKH病的分子免疫机制。

• 批准号: 14571655
• 负责人: HAYAKAWA Kouichi
• 金额: $ 2.18万
• 依托单位: AKITA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571655/
• 关键词: VKH disease; T cell clone; yrosinase family protein; Inflammatory lesions; VKH disease; CSF

We have established T cell clones(TCC) derived from the aqueous humors(AH) and cerebrospinal fluids(CSF) of Vogt-Koyanagi-Harada(VKH) disease patients. The TCCs were established with limiting dilution method after transforming the T cells with Herpesvirus Saimiri(HVS) C488. Four hundred forty four TCCs derived from AH of 3 patients and 958 TCCs from CSF of 7 patients were obtained. Among those TCCs, randomly selected 21 TCCS derived from the AH and 9 TCCs from the CSF of patient 1were challenged with tyrosinase and/or TRP1 peptide mixture. Ten out of 16 CD4 positive TCCs from the AH and 2 out of 6 CD4 positive TCCs from the CSF were reactive against tyrosinase or TRP1. The cytokine production profile showed that all except one of the CD4^+, CD8 TCCs reactive to tyrosinase or TRP were Th1 type. It was strongly suggested that the T cells reactive against tyrosinase and/or TRP1 play an important role to the induction and formation of the inflammatory lesions in VKH disease.

我们已经建立了T细胞克隆（TCC）来源于水性体液（AH）和脑脊液（CSF）的Vogt-Koyanagi-Harada（VKH）病患者。用松鼠猴疱疹病毒（HVS）C488转化T细胞后，用有限稀释法建立TCC。从3例患者的AH和7例患者的CSF中获得了444个TCC和958个TCC。随机选取21例AH来源的TCCs和9例CSF来源的TCCs，用酪氨酸酶和/或TRP 1肽混合物进行攻击。来自AH的16个CD 4阳性TCC中的10个和来自CSF的6个CD 4阳性TCC中的2个对酪氨酸酶或TRP 1具有反应性。细胞因子产生谱显示，除了一个对酪氨酸酶或TRP有反应的CD 4 ^+、CD 8 TCC外，所有TCC都是Th 1型。提示酪氨酸酶和/或TRP 1反应性T细胞在VKH病炎症病变的诱导和形成中起重要作用。

项目成果

Masahiko Y, Kiyoko G, Kunihiko Y.: "Establishment and characterization of T cell clone from aqueous humors and cerebrospinal of Vogt-Koyanagi -Harada disease patients"Akita J Med. 30. 1-11 (2003)

Masahiko Y、Kiyoko G、Kunihiko Y.：“Vogt-Koyanagi-Harada 病患者房水和脑脊髓 T 细胞克隆的建立和表征”Akita J Med。

山口雅彦, 後町清子, 山木邦比古: "rogt-小柳-原田(原田)病患者病巣局所から樹立したT細胞株の解析"秋田医学. 30. 1-11 (2003)

Masahiko Yamaguchi、Kiyoko Gomachi、Kunihiko Yamaki：“从 rogt-Koyanagi-Harada (Harada) 病患者的局部病变中建立的 T 细胞系的分析”秋田医学科学 (Akita Medical Science) 30. 1-11 (2003)。

山口雅彦, 後町清子, 山木邦比古: "γogt-小柳-原田(原田)病患者病巣局所から樹立したT細胞株の解析"Akita J Med. 30. 1-11 (2003)

Masahiko Yamaguchi、Kiyoko Gomachi、Kunihiko Yamaki：“从 γogt-Koyanagi-Harada (Harada) 病患者的局部病变中建立的 T 细胞系的分析”Akita J Med. 30. 1-11 (2003)