Molecular immune mechanisum of VKH disease.

我们建立了从Vogt-Koyanagi-Harada（VKH）病患者的水体液（AH）和脑脊液（CSF）中提取的T细胞克隆（TCC）。用猴猴疱疹病毒（HVS） C488转化T细胞，用极限稀释法建立tcc。3例AH患者获得444例tcc， 7例CSF患者获得958例tcc。在这些tcc中，随机选择21个来自AH的tcc和9个来自患者1的CSF的tcc，用酪氨酸酶和/或TRP1肽混合物攻击。来自AH的16个CD4阳性tcc中有10个，来自CSF的6个CD4阳性tcc中有2个对酪氨酸酶或TRP1有反应。细胞因子生成谱显示CD4^+、CD8 tcc除1个外均为Th1型，对酪氨酸酶或TRP有反应。这强烈提示T细胞对酪氨酸酶和/或TRP1的反应在VKH疾病炎症病变的诱导和形成中起重要作用。

We have established T cell clones(TCC) derived from the aqueous humors(AH) and cerebrospinal fluids(CSF) of Vogt-Koyanagi-Harada(VKH) disease patients. The TCCs were established with limiting dilution method after transforming the T cells with Herpesvirus Saimiri(HVS) C488. Four hundred forty four TCCs derived from AH of 3 patients and 958 TCCs from CSF of 7 patients were obtained. Among those TCCs, randomly selected 21 TCCS derived from the AH and 9 TCCs from the CSF of patient 1were challenged with tyrosinase and/or TRP1 peptide mixture. Ten out of 16 CD4 positive TCCs from the AH and 2 out of 6 CD4 positive TCCs from the CSF were reactive against tyrosinase or TRP1. The cytokine production profile showed that all except one of the CD4^+, CD8 TCCs reactive to tyrosinase or TRP were Th1 type. It was strongly suggested that the T cells reactive against tyrosinase and/or TRP1 play an important role to the induction and formation of the inflammatory lesions in VKH disease.