Molecular immune mechanisum of VKH disease.

我们已经建立了T细胞克隆（TCC）来源于水性体液（AH）和脑脊液（CSF）的Vogt-Koyanagi-Harada（VKH）病患者。用松鼠猴疱疹病毒（HVS）C488转化T细胞后，用有限稀释法建立TCC。从3例患者的AH和7例患者的CSF中获得了444个TCC和958个TCC。在这些TCC中，随机选择21个来自患者1的AH的TCC和9个来自患者1的CSF的TCC，用酪氨酸酶和/或TRP 1肽混合物进行激发。来自AH的16个CD 4阳性TCC中的10个和来自CSF的6个CD 4阳性TCC中的2个对酪氨酸酶或TRP 1具有反应性。细胞因子产生谱显示，除了一个对酪氨酸酶或TRP有反应的CD 4 ^+、CD 8 TCC外，所有TCC都是Th 1型。提示酪氨酸酶和/或TRP 1反应性T细胞在VKH病炎症病变的诱导和形成中起重要作用。

We have established T cell clones(TCC) derived from the aqueous humors(AH) and cerebrospinal fluids(CSF) of Vogt-Koyanagi-Harada(VKH) disease patients. The TCCs were established with limiting dilution method after transforming the T cells with Herpesvirus Saimiri(HVS) C488. Four hundred forty four TCCs derived from AH of 3 patients and 958 TCCs from CSF of 7 patients were obtained. Among those TCCs, randomly selected 21 TCCS derived from the AH and 9 TCCs from the CSF of patient 1were challenged with tyrosinase and/or TRP1 peptide mixture. Ten out of 16 CD4 positive TCCs from the AH and 2 out of 6 CD4 positive TCCs from the CSF were reactive against tyrosinase or TRP1. The cytokine production profile showed that all except one of the CD4^+, CD8 TCCs reactive to tyrosinase or TRP were Th1 type. It was strongly suggested that the T cells reactive against tyrosinase and/or TRP1 play an important role to the induction and formation of the inflammatory lesions in VKH disease.