Analysis of T-cell clone indicating GVL effect due to allo stem cell transplantation and donor lymphocytes infusion.

在t（8 ; 21）急性白血病（AML）急性白血病（AML）患者中，有几份报告演示了对AML 1/MTG 8融合产品的持续检测，代表了最低限度复发性疾病（MRD）。这是一种不受约束的介导机制，它可能会抑制MRD的扩展，并可能导致连续性的结果。这是以前展示的，有些人没有（8：21） AML患者有很高的抗MTG 8抗体称号。MTG 8在正常成人组织中的表达要么不能检测到，要么只能精确检测到。我们制造了MTG 8基因在t（8 ; 21）中的超负荷表达的hypothesis，AML细胞可以作为一种可能的肿瘤抗原产生作用，那么它可能会诱导免疫介导的抑制MRD的表达。我们可以接受HLA-A0201-限制性细胞毒性T-淋巴细胞（CTL）线，反对MTG 8肽（MTG 8baaa 182 -191）使用单细胞衍生的牙科细胞从一个健康的供体。T细胞受体（TCR） Vα17、TCRVβ14和15及TCR ... More Jβ2.1和以前主要用于这些CTL线路。我们的数据是，MTG 8蛋白可能是肿瘤抗原的一个被CTLs识别为T（8 ; 21） AML患者与HLA-A0201谁在长期remission.慢性骨髓瘤白血病（CML）开发在37-year-old man 255天后在alogeneic bone marrow transplantation。patient received a donor lymphocyte infusion （DLI） twice at a dose of 5 x10-D16-D1/kg T cells。他在DLI后完成了完整的细胞遗传反应（CCR） 14周，并在CCR状态下保留了17个月。无论是急性慢性移植与宿主疾病（GVHD）都被观察到。自然杀手（NK）细胞的活动被提升了。此外，对TCR repertoire disclosed oligoclonal of T cell of the TCR Vβ和Jβ subfamilies的分析。这些观察到的证据提供了类似T细胞的克隆扩展的证据，它能够在没有引起GVHD的情况下具有介导的抗微生物活动。Less（低）

Several reports have demonstrated the persistent detection of AML1/MTG8 fusion products, representing minimal residual disease (MRD), in patients with t(8 ; 21) acute myelogenous leukemia (AML) who are in long-term remission. It is probable that immune-mediated mechanisms that are able to suppress the expansion of MRD may result in the continuance of remission. It was previously shown that some t(8 ; 21) AML patients had high anti-MTG8 antibody titers. MTG8 expression in normal adult tissue is either not detectable or else only faintly detectable. We made the hypothesis that the orverexpression of the MTG8 gene in t(8 ; 21) AML cells could act as a possible tumor antigen, which might be able to induce the immune-mediated suppression of the expansion of MRD. We were able to induce HLA-A0201-restricted cytotoxic T-lymphocyte (CTL) lines against an MTG8 peptide (MTG8b aa182-191) using monocyte-derived dendritic cells from a healthy donor. T cell receptor (TCR) Vα17, TCRVβ14 and 15 and TCR … More Jβ2.1 and predominantly used in these CTL lines. Our data which suggest that the MTG8 protein could be one of the tumor antigens recognized by CTLs may be helpful in further investigations of TCR analysis in t(8 ; 21) AML patients with HLA-A0201 who are in long-term remission.Hematologic relapse of chronic myeloid leukemia (CML) developed in 37-year-old man 255 days after allogeneic bone marrow transplantation. The patient received a donor lymphocyte infusion (DLI) twice at a dose of 5x10ィイD16ィエD1/kg T cells. He achieved complete cytogenetic response (CCR) 14 weeks after DLI, and has remained in a CCR state for 17 months. Neither acute chronic graft-versus-host disease (GVHD) was observed. Natural killer (NK) cell activity was elevated. Also, analysis of the TCR repertoire disclosed oligoclonal of T cells of the TCR Vβ and Jβ subfamilies. These observed provide evidence for the clonal expansion of allogeneic T cells that are capable of mediation antileukemic activity without causing GVHD. Less