Study on the kinetics and function of cell adhesion molecule in the generation of skeletal muscle after nerve injury

细胞粘附分子在神经损伤后骨骼肌生成中的动力学及功能研究

• 批准号: 14571717
• 负责人: HATOKO Mitsuo
• 金额: $ 1.79万
• 依托单位: NARA MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571717/
• 关键词: Muscle regeneration; Adhesion Molecule; Cadherin; Catenin; Verve Injury; Skeletal Musle; Rat; Gastrocnemius Muscle; ウエスタンブロット法; 免疫染色法; N-カドヘリン; M-カドヘリン; ウエスタンブロット; 免疫染色

We investigated the expression of cadherin (N,M,R) and catenin (α,β,γ) in the degeneration and regeneration processes after nerve injury using rat sciatic nerve and gastrocnemius model. The expression level and localization were detected by western blotting and immunofluorescent staining. The level of N and R-cadherins had already increased in the ist postoperative week in both groups. In the sutured group, the level returned to almost the same as the control by the 9th postoperative week. In the unsutured group, the N and R-cadherin expression decreased after the 9th postoperative week. The level of M-cadherin expression had already decreased at the 1st postoperative week in both groups. In the unsutured group, this did not subsequenty return to the control level. However, in the sutured group, the level, returned to the control level in the 9th postoperative week. In all cadherins studied, immunofluorescent staining was observed along the muscular membrane and the time course of the … More degree of immunofluorescent staining was similar to the results of western blot analysis. Immunofluorescence was also observed along the muscular membrane in all catenins studied. The level of α and αN-catenins in the sutured group was almost the same as the control in the early period, but after the 4th postoperative week, the level elevated gradually. Subsequently, as reirmervation proceeded, the level decreased and immunofluorescent staining disappeared. β -catenin expression in the unsutured group decreased gradually as denervation of the muscle proceeded and immunofluorescent staining disappeared. Staining in the sutured group for β-catenin was almost the same as the control at all time points studied. That is, immunofluorescene disappeared in the early period after treatment, reappeared as the muscle was reinnervated. The kinetics of the level of γ-catenin in both groups was almost the same as β-catenin. Our study demonstrated that the kinetics of cadherins and catenins, which is thought to be related to the regeneration and reconstitution of skeletal muscle, differ in each protein. This result suggests that each protein plays a different role in the regeneration process of skeletal muscle after nerve injury. Less

以大鼠坐骨神经和腓肠肌为模型，研究了钙粘附素(N，M，R)和连环素(α，β，γ)在神经损伤后退变和再生过程中的表达。Western blotting和免疫荧光染色检测其表达水平和定位。两组患者的N和R-钙粘附素水平在术后第一周均已升高。缝合组在术后第9周恢复到与对照组基本相同。未缝合组术后9周N、R-钙粘蛋白表达减弱。两组患者术后1周M-钙粘蛋白表达水平均已下降。在未缝合组，这一点随后没有恢复到对照水平。而缝合组在术后第9周恢复到对照水平。在所有研究的钙粘附素中，沿着肌膜和…的时间进程观察到免疫荧光染色免疫荧光染色程度较高与免疫印迹分析结果相似。在所有被研究的连环蛋白中，沿着肌膜也观察到了免疫荧光。缝合组术前α、αN-连环蛋白水平与对照组无明显差异，术后4周后逐渐升高。随后，随着再激活的进行，其水平降低，免疫荧光染色消失。未缝合组随着肌肉失神经支配的进行，β-连环素的表达逐渐减少，免疫荧光染色消失。缝合组各时间点β-连环蛋白染色与对照组基本相同。也就是说，免疫荧光在治疗后早期消失，随着肌肉的重新神经支配而重新出现。两组γ-连环蛋白水平的动态变化与β-连环蛋白的变化趋势基本一致。我们的研究表明，钙粘附素和连环素的动力学在每种蛋白质中都不同，它们被认为与骨骼肌的再生和重建有关。这一结果表明，每种蛋白质在神经损伤后骨骼肌再生过程中扮演着不同的角色。较少

项目成果

HATOKO, M et al.: "Expression of N-cadherin by Skeletal Muscle in the Degeneration and the Degeneration/Regeneration Processes after Nerve Injury"Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery. (In press).

HATOKO, M 等人：“神经损伤后变性和变性/再生过程中骨骼肌 N-钙粘蛋白的表达”斯堪的纳维亚整形重建外科和手外科杂志。

MITSUO HATOKO et al.: "Expression of N-Cadherin and αN-Catenin in the Degeneration/Regeneration Process of Rat Skeletal Muscle After Nerve Injury"Journal of Reconstructive Microsurgery. in press.

MITSUO HATOKO 等人：“神经损伤后大鼠骨骼肌变性/再生过程中 N-钙粘蛋白和 αN-连环蛋白的表达”《重建显微外科杂志》出版。

HATOKO, M et al.: "Expression of N-Cadherin and αN-Catenin in the Degeneration/Regeneration Process of Rat Skeletal Muscle After Nerve Injury"Journal of Reconstructive Microsurgery. (In press).

HATOKO, M 等人：“神经损伤后大鼠骨骼肌变性/再生过程中 N-钙粘蛋白和 αN-连环蛋白的表达”《重建显微外科杂志》（正在出版）。

HATOKO, M., et al.: "The Expression of N-cadherin by Skeletal Muscle in the Degeneration and Degeneration/Regeneration Processes after nerve injury"Scandinavian Journal of Plastic and Reconstructive Surgery, and Hand Surgery. (In press).

HATOKO, M. 等人：“神经损伤后变性和变性/再生过程中骨骼肌 N-钙粘蛋白的表达”《斯堪的纳维亚整形与重建外科和手外科杂志》。

HATOKO, M., et al.: "Expression of N-cadherin and αN-catenin in the degeneration/regeneration process of rat skeletal muscle after nerve injury"Journal of Reconstructive Microsurgery. (In press).

HATOKO, M. 等人：“神经损伤后大鼠骨骼肌变性/再生过程中 N-钙粘蛋白和 αN-连环蛋白的表达”《重建显微外科杂志》（正在出版）。