A study on genetic alterations and pioliferative activity of odontogenic tumors

比较基因组杂交（CGH）是一种分子细胞学技术，它允许在单个杂交反应中快速检测整个肿瘤基因组的染色体拷贝数改变。然而，也有一些CGH再调查的染色体改变在牙源性肿瘤。为了明确颌骨成釉细胞瘤染色体拷贝数的改变，应用CGH和荧光原位杂交（FISH）技术对10例颌骨成釉细胞瘤进行了研究。CGH仅在1/10例中检测到染色体改变，包括1q增加和1pter、10q和22q丢失。在这种情况下，使用1号染色体着丝粒探针的FISH分析显示三体细胞显著增加，占所有检查的肿瘤细胞的41.2%。在另一个病例中，检测到三体细胞显著增加（15.4%）。8例中5例可见10-33%的单体细胞。此外，在通过CGH检测到改变的情况下。使用10q26区域特异性探针进行FISH分析，检测到21.4%的单体细胞和22.0%的三体细胞。这些FISH结果与CGH结果一致，表明这些染色体区域的改变可能参与成釉细胞瘤的发病机制。在更多的情况下，使用额外的各种探针进行进一步的研究是必要的。这项研究得到了M博士的支持。Balazs和R. Adany，匈牙利德布勒森大学。正在进行中

Comparative genomic hybridization (CGH) is a molecular cytology technique that allows rapid detection of chromosome copy number alterations through the entire tumor genome in a single hybridization reaction. However, there have been a few CGH repolls of chromosomal alterations in odontogenic tumors. In order to define and map chromosomal copy number alterations in ameloblastomas, which is one of the most popular odontogenic tumors, of the jaw bones, CGH and fluorescence in situ hybridization (FISH) techniques were applied for 10 tumors of this type. Only in 1 out of the 10 cases, chromosomal alterations were detected by CGH ; those included gain at 1q and losses at 1pter, 10q, and 22q. In this case, FISH analysis using chromosome 1 centromeric probe revealed significant increase of trisomic cells, which accounted for 41.2% of all tumor cells examined. Also in another case, significant increase of trisomic cells (15.4%) were detected. Moreover, in 5 out of the rest 8 cases, 10-33% of monosomic cells were observed. Furthermore, in the case in which alterations were detected by CGH. 21.4% of monosonic cells and 22.0% of trisomic cells were detected by FISH analysis using specific probe for 10q26 region. These FISH results, which are well agreed with the CGH results, indicate that the alterations at these chromosomal regions may be involved in pathogenesis of ameloblastomas. Further studies using additional various kinds of probes in much more cases are necessary. This study has been supported by Dr. M. Balazs and Prof. R. Adany, University of Debrecen, Hungary. and it is in progress.