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A study on genetic alterations and pioliferative activity of odontogenic tumors

牙源性肿瘤的基因改变和增殖活性研究

基本信息

批准号：
14571730
负责人：
TOIDA Makoto
金额：
$ 1.47万
依托单位：
Gifu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

Comparative genomic hybridization (CGH) is a molecular cytology technique that allows rapid detection of chromosome copy number alterations through the entire tumor genome in a single hybridization reaction. However, there have been a few CGH repolls of chromosomal alterations in odontogenic tumors. In order to define and map chromosomal copy number alterations in ameloblastomas, which is one of the most popular odontogenic tumors, of the jaw bones, CGH and fluorescence in situ hybridization (FISH) techniques were applied for 10 tumors of this type. Only in 1 out of the 10 cases, chromosomal alterations were detected by CGH ; those included gain at 1q and losses at 1pter, 10q, and 22q. In this case, FISH analysis using chromosome 1 centromeric probe revealed significant increase of trisomic cells, which accounted for 41.2% of all tumor cells examined. Also in another case, significant increase of trisomic cells (15.4%) were detected. Moreover, in 5 out of the rest 8 cases, 10-33% of monosomic cells were observed. Furthermore, in the case in which alterations were detected by CGH. 21.4% of monosonic cells and 22.0% of trisomic cells were detected by FISH analysis using specific probe for 10q26 region. These FISH results, which are well agreed with the CGH results, indicate that the alterations at these chromosomal regions may be involved in pathogenesis of ameloblastomas. Further studies using additional various kinds of probes in much more cases are necessary. This study has been supported by Dr. M. Balazs and Prof. R. Adany, University of Debrecen, Hungary. and it is in progress.

比较基因组杂交(CGH)是一种分子细胞学技术，可以在单个杂交反应中快速检测整个肿瘤基因组中的染色体拷贝数变化。然而，在牙源性肿瘤中也有一些关于染色体改变的CGH重测。为了明确和定位颌骨中最常见的牙源性肿瘤之一成釉细胞瘤的染色体拷贝数变化，应用CGH和荧光原位杂交(FISH)技术对10例此类肿瘤进行了研究。10例患者中仅1例在CGH检查中发现染色体改变，包括1q增加，1p、10q和22q丢失。在本例中，使用1号染色体着丝粒探针的FISH分析显示，三体细胞显著增加，占所检测的所有肿瘤细胞的41.2%。另1例三体细胞明显增多(15.4%)。此外，在其余8例中，有5例观察到10-33%的单体细胞。此外，在CGH检测到变化的情况下。用10q26区特异性探针进行FISH分析，可检测到21.4%的单体细胞和22.0%的三体细胞。FISH结果与CGH结果一致，提示这些染色体区域的改变可能与成釉细胞瘤的发病有关。在更多的情况下使用额外的各种探针进行进一步的研究是必要的。这项研究得到了匈牙利德布勒森大学M.Balazs博士和R.Adany教授的支持。而且它正在进行中。