Studies on the basement membranes below the placodes in head-The model of the basement membrane of the tooth germ.

头部基板下方基底膜的研究——牙胚基底膜模型。

• 批准号: 14571742
• 负责人: OSAWA Takuji
• 金额: $ 2.24万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571742/
• 关键词: Placode; Amphibian; Basement membrane; Hemidesmosome; Anchoring fibril; Collagen fibril; Lamina densa; Ectoderm

The morphological changes of the basement membranes below placodes and the underlying collagen fibrils during development were studied with electron microscopy, using amphibian as materials. Epidermal basement membrane and the collagen fibrils in the body wall of Cucumaria frondosa were also studied to elucidate the basic conformation of the basement membrane, and to make the process of the formation of collagen layer clear.The polygonal framework, which was similar to the hypothesis by Yurchenco (1986), was found in the epidermal basement membrane of Cucumaria. We were possibly successful to show the basic framework with type IV collagen. Similar morphologies have been seen in the epidermal basement membranes of Rana during metamorphosis, and also in the basement membranes of mouse and chick during development.In the present study, it became clear that the arrangement of the collagen fibrils in the body wall of echinoderms differed from those in the mammalian dermis. The data in the present study can be thought as the basic information to the studies of the basement membrane of the tooth germ.

以两栖动物为材料，利用电子显微镜研究了发育过程中基板下方基底膜及其下胶原纤维的形态变化。为了阐明黄瓜属植物表皮基底膜的基本结构和胶原纤维的形成过程，还对黄瓜属植物表皮基底膜和体壁中的胶原纤维进行了研究，发现黄瓜属植物表皮基底膜中存在与Yurchenco（1986）假说相似的多边形骨架。我们可能成功地显示了IV型胶原的基本框架。相似的形态已被视为在表皮基底膜的蛙在变态过程中，也在基底膜的小鼠和鸡在development.In本研究中，很明显，胶原纤维的安排在体壁棘皮动物不同于那些在哺乳动物真皮。本研究为牙胚基底膜的研究提供了基础资料。

项目成果

Tokuji Osawa et al.: "Measurement of the repeat period of myelin sheath using ultrathin frozen sections"Journal of Electron Microscopy. 51・3. 195-197 (2002)

Tokuji Osawa 等：“使用超薄冷冻切片测量髓鞘的重复周期”，电子显微镜杂志 51・3（2002 年）。

Tokuji Osawa: "Regeneration of the epidermis and mucosal epithelium on the basement membranes."Medical Electron Microscopy. 36・4. 193-203 (2003)

大泽德司：“基底膜上的表皮和粘膜上皮的再生”。医学电子显微镜36・4（2003）。

Yohko Kato, et al.: "Solubilization of organic matrix of Lingula unguis with chitinase."Biomineralization. in press. (2004)

Yohko Kato 等人：“用几丁质酶溶解舌拉有机基质。”生物矿化。

Ichiro Naito, Tokuji Osawa, et al.: "Fine structural localization of collagen type IV α chains in the rat glomerular basement membrane using chain-specific monoclonal antibodies."Current Progress in Human Life Science. in press. (2004)

Ichiro Naito、Tokuji Osawa 等人：“使用链特异性单克隆抗体对大鼠肾小球基底膜中 IV 型胶原蛋白链进行精细结构定位。”《人类生命科学最新进展》（2004 年）。

Tokuji Osawa et al.: "Real electron microscopic images of collagen fibrils in the endonerium"Journal of Electron Microscopy. 51・5. 341-345 (2002)

Tokuji Osawa 等人：“内膜中胶原纤维的真实电子显微镜图像”电子显微镜杂志 51・5（2002 年）。