Molecular switch between cell proliferation and differentiation in the craniofacial development

颅面发育中细胞增殖和分化之间的分子转换

基本信息

批准号：
14571745
负责人：
AMANO Osamu
金额：
$ 1.92万
依托单位：
Meikai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

Heat shock proteins (Hsps) are induced by heat shock and other physiological stresses, and protect cells from cell death through their roles as molecular chaperone. Hsps are constitutively expressed in the cells under unstressed conditions. The 27kDa Hsp (Hsp27) has been suggested to work at crucial cellular checkpoints for proliferation, and differentiation, and cell death. Hsp27 was suggested to regulated the acinar cell differentiation and protect them from apoptosis.In the present study, we have examined the chronological immunolocalization of Hsp27 in the regenerating acinar cells after release of duct ligation in the rat submandibular gland.Male Wister rats of 8 weeks old were anesthetized and the right main excretory duct of submandibular gland were ligated by small clips and the left is control. After 1 week, clips were removed to release the duct ligation. Submandibular glands were fixed with 4% paraformaldehyde solution after 0, 1, 3, 5, 7, 10, 14 and 28 days after clip remov … More al. They were treated for immunohistochemistry of Hsp27 and PCNA.In the control and normal submandibular glands, Hsp27-immunoreactivity was observed in the nerves and blood vessels but not in the acinar and duct cells. Three days after the clip removal, small number of Hsp27-immunopositive cells was found in the distal end of regenerating acinar portion. Hsp27-immunoreacitvity was intensely localized in the cytoplasm of small and round-shaped epithelial cells. Hsp27-positive cells were increased in number until 7 days after clip removal, but no positive cells were recognized in the acini after 14 days of clip removal. Histologically, normal acinar cells were recognized after 14 days. Hsp27-positive epithelial cells were not colocalized with PCNA-immunoreactivity during the acinar regeneration.These results suggest that differentiation of acinar cells occur during 3-7 days after clip removal, and Hsp27 regulates the initial differentiation of acinar cells during submandibular gland regeneration. Less

热休克蛋白（HSP）是由热休克和其他物理应力诱导的，并通过其作为分子链酮的作用来保护细胞免受细胞死亡的影响。 HSP在无重大条件下在细胞中组成型表达。已建议27KDA HSP（HSP27）在关键的细胞检查点上工作，以进行增殖，分化和细胞死亡。 Hsp27 was suggested to regulate the acinar cell differentiation and protect them from apoptosis.In the present study, we have examined the chronological immunolocalization of Hsp27 in the regenerating acinar cells after release of duct ligation in the rat submandibular gland.Male Wister rats of 8 weeks old were anesthetized and the right main extreme duct of submandibular gland were ligated by small clips and the left是控制。 1周后，删除夹子以释放管道连接。下颌下腺在0、1、3、5、7、10、14和28天后用4％多聚甲醛溶液固定夹子溶液……更多。他们接受了HSP27和PCNA的免疫组织化学处理。在对照和正常的下颌网格中，在神经和血管中观察到Hsp27-免疫反应性，但在腺泡和管道细胞中未观察到。剪辑去除后三天，在再生腺泡部分的远端发现了少量的Hsp27-免疫阳性细胞。 HSP27-免疫性抗毒性在小型和圆形上皮细胞的细胞质中强烈定位。 Hsp27阳性细胞的数量增加到夹子去除后7天，但是在去除夹子14天后，在ACINI中未识别阳性细胞。在组织学上，14天后识别正常腺泡细胞。 Hsp27阳性上皮细胞在腺泡再生过程中未与PCNA-免疫反应性共定位。这些结果表明，腺泡细胞的分化发生在夹夹去除后的3-7天内，HSP27调节在下细胞腺体再生过程中腺泡细胞的初始分化。较少的