Histochemial and Mollecular Biogical Studies on Functional Diffentiation of Chondrcytes

软骨细胞功能分化的组织化学和分子生物学研究

• 批准号: 14571747
• 负责人: TAJIMA Yoshifumi
• 金额: $ 1.6万
• 依托单位: Meikai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571747/
• 关键词: Chondrocytes; Fuctional differentiation; Molecular biology; 分子生物学; 機能的分化; 病態

In this research project, we aimed our interest to investigate modulation of cartilage cells function in accordance with their growth and differentiation. To confirm this research object, we used immunohistochemial and molecular biological procedures on a variety of cartilage cells and tissues from mandibular condyle and growth plate. We obtaind the following results :1)Estrogen affects the cartilage cell maturation via a regulation of their PTH/PTHP receptor in the rat mandibular condylar cartilage.2)A certain GFP-tagged protein such as MT1-MMP can be utilized as a functional marker of chodrocytic growth and differentiation.

在这个研究项目中，我们的目的是研究软骨细胞功能的调节与它们的生长和分化。为了证实这一研究对象，我们采用细胞化学和分子生物学方法对各种下颌骨髁状突和生长板的软骨细胞和组织进行了研究。结果表明：1）雌激素通过调节大鼠髁突软骨细胞的PTH/PTHP受体来影响软骨细胞的成熟; 2）某些GFP标记的蛋白如MT 1-MMP可以作为软骨细胞生长和分化的功能性标志物。

项目成果

Y Tajima et al.: "Persistence and modulation of PTH/PTHrP receptor expression in hypertrophic cells of mandibular condylar cartilage"Acta Histochem Cytochem. 36・(5). 481-487 (2003)

Y Tajima 等：“下颌髁软骨肥大细胞中 PTH/PTHrP 受体表达的持久性和调节”Acta Histochem Cytochem 36·(5) 481-487 (2003)。

Y.Tajima, M.Kuroda, J.Okazawa, K.Kusama, K.Kurihara: "Persistence and modulation of PTH/PTHrP receptor expression in hypertrophic cells of mandibular condylar cartilage from ovariectomized and estrogen-treated ovariectomized adult rats."Acta Histochem. Et

Y.Tajima、M.Kuroda、J.Okazawa、K.Kusama、K.Kurihara：“卵巢切除和雌激素治疗的卵巢切除成年大鼠下颌髁软骨肥大细胞中 PTH/PTHrP 受体表达的持续性和调节。”Acta Histochem

T Takuma et al.: "Trafficking of GFP-tagged SNARE proteins in HSY cells"J Biochem. 132. 729-735 (2002)

T Takuma 等人：“HSY 细胞中 GFP 标记的 SNARE 蛋白的运输”J Biochem。

Y Tajima et al.: "Persistence and modulation of PTH/PTHrP receptor expression in hypertrophic cells of mandibular condylar cartilage"Acta Histochem Cytochem. 36(5). 481-487 (2003)

Y Tajima 等：“下颌髁软骨肥大细胞中 PTH/PTHrP 受体表达的持久性和调节”Acta Histochem Cytochem。

田島義文: "病理・口腔病理 サブノート(第3版)"サンショウ(株). 77 (2003)

田岛芳文：《病理学/口腔病理学分注（第3版）》Sansho Co., Ltd. 77（2003）