Histochemical and Cytochemical Studies on Functional Differentiation of the Chondrocytes

软骨细胞功能分化的组织化学和细胞化学研究

• 批准号: 07671983
• 负责人: TAJIMA Yoshifumi
• 金额: $ 1.47万
• 依托单位: Meikai University School of Dentistry
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671983/
• 关键词: Chondrocytes; Functional differentiation; Histochemistry; 分化と機能

In this research project, we addressed our interest on the mechanical modulation of the cartilage cell (s) function in accordance with their growth and differentiation. To investigate their mechanism, we studied mandibular condylar and articular cartilages by means of histochemical procedures using a variety of marker for the cartilage cell function. We used the cartilages from hypophysectomized- and ovariectomized rats.The following results were obtained :(1) Growth hormone affects the process of cartilage cell glycosylation and subsequent sulfation occurring in the Golgi complexes.(2) Growth hormone specifically promotes the proliferation phase of the chondrocytes in the proliferative zone in vivo.(3) Estrogen deficiency at early stages after ovariectomy causes cell proliferation and matrix synthesis in the growth plate cartilage.

在这项研究中，我们对软骨细胞(S)生长和分化过程中的机械调节功能感兴趣。为了探讨它们的机制，我们利用软骨细胞功能的各种标记物，通过组织化学方法研究了下颌骨的髁状突和关节软骨。结果表明：(1)生长激素影响软骨细胞糖基化和随后在高尔基复合体中发生的硫酸盐化过程；(2)生长激素特异性地促进体内增殖区软骨细胞的增殖期；(3)卵巢切除后早期雌激素缺乏导致生长板软骨细胞增殖和基质合成。

项目成果

Y Tajima et al.: "In vivo modulation of proliferating cell nuclear antigen in growth plate chondrocytes-----" J Histochem Cytochem. 44. 713-720 (1996)

Y Tajima 等人：“生长板软骨细胞中增殖细胞核抗原的体内调节 -----”J Histochem Cytochem。

Y Tajima et al.: "In vivo modulation of proliferating cell nuclear antigen in growth plate chondrocytes" J Histochem Cytochem. 44. 713-720 (1996)

Y Tajima 等人：“生长板软骨细胞中增殖细胞核抗原的体内调节”J Histochem Cytochem。

Y. Tajima et al.: "Ultrastructural demonstration of soybean aggulutinin binding sites in rat mandibular condylar chondrocytes" Jpn. J. Oral Biol.38. 132-138 (1996)

Y. Tajima 等：“大鼠下颌髁突软骨细胞中大豆凝集素结合位点的超微结构证明”Jpn。

Tajima Y, Inaba M, Utsumi N, Yokose S.: "Ultrastructural demonstration of SBA binding sites in rat mandibular condylar chondrocytes." Jpn I Oral Biol. 38. 132-138 (1996)

Tajima Y、Inaba M、Utsumi N、Yokose S.：“大鼠下颌髁突软骨细胞中 SBA 结合位点的超微结构演示。”

Tajima Y, Kato K, Maruyama S, Hosoi K.: "In vivo modulation of proliferating cell nuclear antigen in growth plate chondrocytes from normal, hypophysectomized, and growth hormone-treated hypophysectomized rats." J Histochem Cytochem.44. 713-720 (1996)

Tajima Y、Kato K、Maruyama S、Hosoi K.：“正常、垂体切除和生长激素治疗的垂体切除大鼠的生长板软骨细胞中增殖细胞核抗原的体内调节。”