CENTRAL CONTROL BY CIRCUMVENTRICULAR ORGANS OF SALIVARY SECRETION

心室周围器官对唾液分泌的中枢控制

• 批准号: 14571769
• 负责人: IKENAGA Kiyotoshi
• 金额: $ 1.92万
• 依托单位: KYUSHUDENTAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571769/
• 关键词: Salivary secretion; parotid saliva; thirst; osmorceptor; sodium sensing; circumventricular organ; acetylcholine; rat

The number of people who claim oral dryness increases recently. Although this is due to the increase of elderly persons and stress and a change of life style, the underlying mechanisms are not well-known. The saliva is made by the blood and the secretion is easily influenced by osmolarity and volume of body fluid and by the autonomic nervous system. This study was designed to investigate how the subfornical organ (SFO), which is a center of body fluid balance and cardiovascular regulation, is involved in salivary secretion. Rats were used for in vivo and in vitro experiments. In the in vivo experiments, a change of drinking and parotid saliva was measured by intracerebroventricular injection hypertonic saline and sucrose. We found the induction of drinking and the reduction of salivary secretion were larger by the hypertonic saline than by the sucrose. These results indicate that the salivary secretion is affected by central detection of osmolarity and sodium ions. In vitro experiments, we found the existence of sodium ion sensing receptors in the SFO and the activation of SFO neurons by a certain concentration of sodium ions. Further, we found the existence of acetylcholine muscarinic and nicotinic receptors in the SFO and the involvement of them in the body fluid balance.

最近，声称口干的人数有所增加。虽然这是由于老年人的增加和压力以及生活方式的改变，但其潜在的机制尚不清楚。唾液是由血液产生的，唾液的分泌很容易受到渗透压和体液体积以及自主神经系统的影响。本研究旨在研究穹隆下器(SFO)如何参与唾液分泌，SFO是体液平衡和心血管调节的中心。用大鼠进行体内和体外实验。在体内实验中，通过侧脑室注射高渗盐水和蔗糖来测量饮水和腮腺唾液的变化。高渗盐水对小鼠饮酒的诱导作用和唾液分泌量的减少均大于蔗糖。这些结果表明，唾液分泌受到渗透压和钠离子中枢检测的影响。在体外实验中，我们发现在SFO中存在钠离子感受器，并且一定浓度的钠离子可以激活SFO神经元。此外，我们还发现SFO中存在乙酰胆碱、M受体和烟碱受体，并参与体液平衡。

项目成果

K.Ito, M.Morikawa, K.Inenaga: "Suppression of reflex saliva from rat parotid gland following intracerebroventricular injection of hypertonic NaCl and sucrose"Archeues of Oral Biology. 47(1). 93-97 (2002)

K.Ito、M.Morikawa、K.Inenaga：“脑室内注射高渗氯化钠和蔗糖后大鼠腮腺唾液反射的抑制”口腔生物学的Archeues。

Ono K, Honda E, Toyono T, et al.: "Evidence for the presence of nicotinic receptors on rat subfornical organ neurons"Autonomie Neuroscience. 108. 87-90 (2003)

Ono K、Honda E、Toyono T 等人：“大鼠穹窿下器官神经元上存在烟碱受体的证据”Autonomie Neuroscience。

Honda E, Ono K, Inenaga K.: "DAMGO suppresses both excitatory and inhibitory synaptic transmission in supraoptic neurones of mouse hypothalamic slice preparations"J.Neuroendocrinology. (in press).

Honda E、Ono K、Inenaga K.：“DAMGO 抑制小鼠下丘脑切片制剂的视上神经元的兴奋性和抑制性突触传递”J.Neuroendocrinology。

Honda E, Ono K, Toyono T, Kawano H, Masuko S, Inenaga K.: "Activation of muscarinic receptors in rat subfornical organ neurones"J.Neuroendocrinol.. 15. 770-777 (2003)

Honda E、Ono K、Toyono T、Kawano H、Masuko S、Inenaga K.：“大鼠穹窿下器官神经元中毒蕈碱受体的激活”J.Neuroendocrinol.. 15. 770-777 (2003)

Xu S-H, Ono K, Honda E, Inenaga K: "Noncholinergic actions of atropine on GABAergic synaptic transmission in the subfornical organ of rat slice preparations"Toxicology and Applied Pharmacology. 178. 180-185 (2002)

Xu S-H、Ono K、Honda E、Inenaga K：“阿托品对大鼠切片制剂穹窿下器官 GABA 突触传递的非胆碱能作用”毒理学和应用药理学。