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Changes of Serum Lipid Levels induced by Periodontal Infection in Rats

牙周感染引起的大鼠血清脂质水平的变化

基本信息

批准号：
14571809
负责人：
ITAGAKI Yumi
金额：
$ 2.18万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

In recent years, some evidence has been presented that serum antibody titer against Porphyromonas gingzvalis(Pg), cholesterol, triglyceride and blood glucose levels were significantly higher in periodontally diseased patients than in healthy subjects. Thus, it is suggested that periodontal disease could influence the metabolism of lipids and glucose. This study examined the effects of experimentally induced periodontitis on the serum level of triglyceride, glucose, cytokine(TNF-α) and C-reactive protein(CRP) in rats.Twenty-nine female Wistar rats were divided into the following 3 groups : Pg-infected group(n=9), Pg-non infected group(n=8) and control group(n=12). Experimental periodontitis was induced by applying a small elastic ring around the mandibular first molar(M1) in Pg-infected and non infected groups. After one week, Pg soaked or sterile silk sutures were inserted into periodontal pockets and fixed by elastic rings around M1 of rats in the Pg-infected or non infected group, re … More spectively. Silk sutures and elastic rings were changed after 2 weeks. Two more weeks after changing the sutures and elastic rings, rats were sacrificed by drawing blood from the hearts and removed mandibular bones. Rats of control group were not treated and maintained for 5 weeks.Significant alveolar bone loss was found in the Pg-infected (2.20±0.14mm) and non infected (2.14±0.13mm) groups compared with that in the control group (1.36±0.11mm). However, there was no significant difference between Pg-infected and non infected groups. The blood glucose level was significantly higher in the Pg-infected group(143.1±6.1mg/dl) than that in the Pg-non infected (132.3±4.8mg/dl) and control groups (132.7±9.4mg/dnl). With regard to triglyceride, there was no significant difference in the three groups because of large individual differences. TNF-α levels of all groups were below the detectable level(<0.7pg/ml). There was no significant difference in the CRP level.It was considered that Pg-infection of periodontium could raise the blood glucose level. However, TNF-α, which has been considered involved in the increased glucose level, was not detected in peripheral blood. Further studies are needed to determine the mechanisms underlying the association between Pginfection of the periodontium and an increase in the glucose level. Less

近年来，有证据表明牙周病患者血清抗牙龈卟啉单胞菌（Pg）抗体滴度、胆固醇、甘油三酯和血糖水平明显高于健康人。因此，我们认为牙周病可能影响脂质和葡萄糖的代谢。本研究探讨实验性牙周炎对大鼠血清甘油三酯、葡萄糖、细胞因子（TNF-α）和c反应蛋白（CRP）水平的影响。29只雌性Wistar大鼠分为3组：pg感染组（n=9）、pg未感染组（n=8）和对照组（n=12）。在pg感染组和未感染组的下颌第一磨牙（M1）周围应用小弹性环诱导实验性牙周炎。1周后，将Pg浸泡或无菌丝缝线插入牙周袋，用弹力环固定在Pg感染组和未感染组大鼠的M1周围。2周后更换丝缝线和弹力环。在更换缝合线和弹性环两周后，通过从心脏抽血和移除下颌骨来处死大鼠。对照组不给药，维持5周。与对照组（1.36±0.11mm）相比，pg感染组（2.20±0.14mm）和未感染组（2.14±0.13mm）的牙槽骨丢失明显。然而，pg感染组与未感染组之间无显著差异。pg感染组血糖水平（143.1±6.1mg/dl）明显高于未感染组（132.3±4.8mg/dl）和对照组（132.7±9.4mg/dnl）。在甘油三酯方面，由于个体差异较大，三组间无显著差异。各组TNF-α水平均低于检测水平（<0.7pg/ml）。两组CRP水平无显著差异。认为牙周组织感染可引起血糖升高。然而，被认为与葡萄糖水平升高有关的TNF-α在外周血中未被检测到。需要进一步的研究来确定牙周组织感染与葡萄糖水平升高之间的关联机制。少