Gene expressions of Cross-talking of transcription factors controlling development of oral-maxillofacial region

口腔颌面部的发育受多种生长因子的控制。最近，核心结合蛋白-1 （CBFA-1）被证明是成骨细胞分化的关键转录调节因子，该基因的突变导致锁骨颅发育不良。CBFA-1深入参与口腔颌面区发育和牙齿形成。骨形态发生蛋白（BMPs）最初被鉴定为诱导异位骨形成的蛋白。最近的研究表明，bmp是一种多功能生长因子，可调节细胞增殖和细胞外基质的合成，并在牙齿发育过程中作为上皮-间质相互作用的信号。近年来的研究表明，BMP可诱导CBFA-1基因的表达。因此,。研究了BMP和CBFA-1在小鼠胚胎中的定位和基因表达。mRNA的分布和定位是共定位的。第12天颅骨前鼻区间充质凝聚物中CBFA-1 mRNA的表达增多。这些区域的基因表达从E13和E14天开始扩散。在沿梅克尔软骨的成骨细胞中检测到CBFA-1基因的表达。0 ~ 21 d时，在上颌骨和下颌骨成骨细胞中检测到强烈的基因表达。在牙胚芽期，基因表达定位于牙芽和牙毛囊间充质细胞。杯状期，牙釉质器官和初代牙釉质结有强烈的表达。基因表达转移到牙乳头和牙滤泡。次生牙釉质结表现出较强的基因表达。BMP-4的mRNA分布和定位与CBFA-1共定位。在骨形成过程中，BMP-4定位于成骨前细胞。这些数据表明BMP-4与成骨细胞的早期分化有关，而CBFA-1与成骨细胞的成熟有关。少

Development of oral-maxillofacial region is controlled by various growth factors.Recently, core binding protein-1 (CBFA-1) was shown to be a critical transcriptional regulator of osteoblasts differentiation, Mutation in this gene causes cleidocranial dysplasia. CBFA-1 is deeply involved in formation of development of oral-maxillofacial region and tooth formation. Bone morphogenetic proteins (BMPs) were originally identified as proteins that induce ectopic bone formation. More recent studies have shown that BMPs are a multifunctional growth factor that regulates cell proliferation and the synthesis of extracellular matrices and acts as a signal in epithelial-mesenchymal interaction during tooth development. In the recent studies, it was demonstrated that gene expression of CBFA-1 is induced by BMP. Therefore,. localizations and gene expressions of BMP and CBFA-1 were investigated in mouse embryos.The distributions of mRNA and localization were co-localized. The expressions CBFA-1 mRNA w … More ere detected in the mesenchymal condensations of front nasal regions of skull at E12 days. The gene expressions of these regions were spread from E13 and E14 days. Gene expressions CBFA-1 were detected in the osteoblasts along the Meckel' s cartilage. From 0 to 21 days the strong gene expressions were detected in the osteoblasts of maxilla and mandible.In the tooth germs of the bud stage, gene expressions were localized in the tooth buds and the mesenchymal cells of the dental follicle. In the cup stage, strong expressions were detected in the enamel organ and the primary enamel knots. Gene expressions were trans-localized to the dental papilla and dental follicle. The secondary enamel knots showed strong gene expression.The distributions of mRNA and localization of BMP-4 were co-localized with CBFA-1. During bone formation, BMP-4 was localized in the preosteoblasts.These data show that BMP-4 was related in the early differentiation of osteoblasts but CBFA-1 related maturation of osteoblasts. Less