Gene expressions of Cross-talking of transcription factors controlling development of oral-maxillofacial region

控制口颌面部发育的转录因子串扰的基因表达

• 批准号: 14571866
• 负责人: SATO Yoshinori
• 金额: $ 2.24万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571866/
• 关键词: Oral-maxillofacial region; BMP; CBFA-1; mandible; maxilla; 骨形成; RNAKL; OGF; 軟骨; 顎顔面の発育; mRNA; Meckel軟骨

Development of oral-maxillofacial region is controlled by various growth factors.Recently, core binding protein-1 (CBFA-1) was shown to be a critical transcriptional regulator of osteoblasts differentiation, Mutation in this gene causes cleidocranial dysplasia. CBFA-1 is deeply involved in formation of development of oral-maxillofacial region and tooth formation. Bone morphogenetic proteins (BMPs) were originally identified as proteins that induce ectopic bone formation. More recent studies have shown that BMPs are a multifunctional growth factor that regulates cell proliferation and the synthesis of extracellular matrices and acts as a signal in epithelial-mesenchymal interaction during tooth development. In the recent studies, it was demonstrated that gene expression of CBFA-1 is induced by BMP. Therefore,. localizations and gene expressions of BMP and CBFA-1 were investigated in mouse embryos.The distributions of mRNA and localization were co-localized. The expressions CBFA-1 mRNA w … More ere detected in the mesenchymal condensations of front nasal regions of skull at E12 days. The gene expressions of these regions were spread from E13 and E14 days. Gene expressions CBFA-1 were detected in the osteoblasts along the Meckel' s cartilage. From 0 to 21 days the strong gene expressions were detected in the osteoblasts of maxilla and mandible.In the tooth germs of the bud stage, gene expressions were localized in the tooth buds and the mesenchymal cells of the dental follicle. In the cup stage, strong expressions were detected in the enamel organ and the primary enamel knots. Gene expressions were trans-localized to the dental papilla and dental follicle. The secondary enamel knots showed strong gene expression.The distributions of mRNA and localization of BMP-4 were co-localized with CBFA-1. During bone formation, BMP-4 was localized in the preosteoblasts.These data show that BMP-4 was related in the early differentiation of osteoblasts but CBFA-1 related maturation of osteoblasts. Less

口腔颌面部的发育受多种生长因子的调控，核心结合蛋白-1（core binding protein-1，CBFA-1）是成骨细胞分化的关键转录调控因子，该基因突变可导致锁骨颅骨发育不良。CBFA-1与口腔颌面部的发育和牙齿的形成密切相关。骨形态发生蛋白（BMP）最初被鉴定为诱导异位骨形成的蛋白质。最近的研究表明，骨形成蛋白是一种多功能的生长因子，调节细胞增殖和细胞外基质的合成，并在牙齿发育过程中作为上皮-间充质相互作用的信号。近年来的研究表明，BMP可诱导CBFA-1基因的表达。因此，。研究了BMP和CBFA-1在小鼠胚胎中的定位和基因表达，mRNA的分布和定位是共定位的。CBFA-1 mRNA的表达与CBFA-1 mRNA的表达呈负相关。 ...更多信息 在E12天，在颅骨前鼻区的间充质致密物中检测到。这些区域的基因表达从E13和E14开始扩散。CBFA-1基因表达在沿着Meckel软骨的成骨细胞中检测到。0 ~ 21 d，上颌骨成骨细胞中有较强的基因表达，芽期牙胚中，基因表达定位于牙芽和牙囊间充质细胞中。在杯状期，釉器和初级釉结中检测到强烈的表达。基因表达在牙乳头和牙囊中有反式定位。次级釉节中有较强的基因表达，BMP-4的mRNA和定位与CBFA-1共定位。在成骨过程中，BMP-4定位于前成骨细胞中，提示BMP-4与成骨细胞的早期分化有关，而CBFA-1与成骨细胞的成熟有关。少

项目成果

稲毛稔彦他: "歯周組織とエナメルタンパク"永末書店. 61-69 (2002)

Toshihiko Inage 等人：“牙周组织和牙釉质蛋白”，Nag​​asue Shoten 61-69 (2002)。

稲毛稔彦 他: "歯周組織とエナメルタンパク"永末書店. 61-69 (2002)

Toshihiko Inage 等人：“牙周组织和牙釉质蛋白”，Nag​​asue Shoten 61-69 (2002)。

稲毛稔彦, 大井田新一郎, 他: "抜歯窩の治癒過程におけるBMPの遺伝子発現"日本大学歯学部総合歯学研究所研究報告書. 03号. 101-111 (2003)

Toshihiko Inage、Shinichiro Oida等人：“拔牙槽愈合过程中BMP基因的表达”日本大学牙科学院牙科研究所03号101-111(2003)。

稲毛稔彦, 他: "歯胚の成長に伴う硬組織形成の微細構造"日本大学歯学部研究紀要(一般教育). 29号. 45-53 (2001)

Toshihiko Inage 等人：“与牙胚生长相关的硬组织形成的精细结构”日本大学牙科学院通报（普通教育）29, 45-53 (2001)。

稲毛稔彦, 佐藤吉則, 大井田新一郎他: "マウス顎顔面の発育におけるCBFA-1およびBMP-4の局在について"日大歯学. (発表予定). (2003)

Toshihiko Inage、Yoshinori Sato、Shinichiro Oida 等人：“CBFA-1 和 BMP-4 在小鼠颌面发育中的定位”日本大学牙科（计划发表）。