A study of the Calcification by matrix vesicles in closure of immature teeth

基质囊泡钙化在未成熟牙闭合过程中的研究

• 批准号: 14571961
• 负责人: MAKI Kenshi
• 金额: $ 1.66万
• 依托单位: Kyushu Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571961/
• 关键词: Odontollast; Matrix Vesicle; Protease; Lactate Dehydrogenate; Matrix Vesicle; 歯根膜; protease; Metalloprotease; Alkaline phosphataes

Matrix vesicles are extracellular organelles that initiate mineralization in the extracellular that initiate mineralization in the extracellular matrix of carious calcifying tissues. The matrix vesicles fractions from the dentin of fetal bovine, obtained by collagenase digestion and differential centrifugation, were subjected to sucrose-density-gradient centrifugation.In this report, we investigated the enzymes alkaline phosphates activity( ALP), protease activity lactate dehydrogenase(LDH) and protease to evaluate the matrix vesicles. We found not only matrix vesicles but also matrix vesicle like vesicles without protease in the dentin of fetal bovine. Protease activity after inhibition with o-phenanthroline was restored by the addition of Co<2+>, A1<2+>, Zn<2+>, Mg<2+>,. These results show that the protease in the matrix vesicles in the dentin is a metalloprotease.

基质囊泡是细胞外细胞器，其启动细胞外矿化，启动龋齿钙化组织的细胞外基质矿化。通过胶原酶消化和差速离心获得胎牛牙本质基质囊泡组分，进行蔗糖密度梯度离心。在本报告中，我们通过检测碱性磷酸酶活性（ALP）、蛋白酶活性、乳酸脱氢酶（LDH）和蛋白酶活性来评估基质囊泡。我们在胎牛牙本质中不仅发现了基质囊泡，而且还发现了不含蛋白酶的基质囊泡样囊泡。用邻菲咯啉抑制后的蛋白酶活性通过添加Co 2+ 、A1 2+ 、Zn 2+ 、Mg 2+ 恢复。这些结果表明牙本质基质囊泡中的蛋白酶是金属蛋白酶。