The mechanism of immature permanent teeth's apex closure

未成熟恒牙根尖闭合的机制

• 批准号: 12672008
• 负责人: MAKI Kenshi
• 金额: $ 1.6万
• 依托单位: Kyushu Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12672008/
• 关键词: matrix vesicles; Alkaline phosphatase; Lactate dehydrogenase; protease; Matrix Vesicles; Lactate dehydrogenase; Alkaline phosphatase

The dentin was removed from bovine tooth germs, followed by the separation of the intracellular matrix vesicles fraction after collagenase treatment.Alkaline phosphatase activity, protease activity, and lactate dehydrogenase activity were assayed for the marker enzyme of matrix vesicles. Matrix vesicles containing alkaline phosphatase, metalloprotease, and lactate dehydrogenase were found in the dentin. We also found matrix vesicle-like vesicles with a high density. Those also contained alkaline phosphatase and lactate dehydrogenase, but not metalloprotease.

从牛牙胚中去除牙本质，然后分离胶原酶处理后的细胞内基质囊泡组分，测定基质囊泡的标记酶碱性磷酸酶活性、蛋白酶活性和乳酸脱氢酶活性。基质囊泡含有碱性磷酸酶，金属蛋白酶，和乳酸脱氢酶被发现在牙本质。我们还发现了高密度的基质囊泡样囊泡。这些还含有碱性磷酸酶和乳酸脱氢酶，但不含金属蛋白酶。

项目成果

Maki K, Hayashi S, Nishioka T, Kimura M, Noguchi T: "A New Type of Matrix Vesicles is present in Bovine Tracheal cartlages"Connect Tissue Res.. 41(2). 109-115 (2000)

Maki K、Hayashi S、Nishioka T、Kimura M、Noguchi T：“牛气管软骨中存在一种新型基质囊泡”Connect Tissue Res.. 41(2)。

K.Maki,S.Hoyashi,T.Niohioka,K.Kimura,T.Nogachi.: "A new Type of Matrix Vesicles is found in Fetal Bovine Tracheal Cartilages"Conrective Tissue Research.. 41(2). 109-115 (2000)

K.Maki，S.Hoyashi，T.Niohioka，K.Kimura，T.Nogachi.：“在胎牛气管软骨中发现一种新型基质囊泡”结缔组织研究.. 41（2）。