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Accumulation of Bisphenol A in the body

双酚A在体内蓄积

基本信息

批准号：
14571964
负责人：
TANAKA Mitsuro
金额：
$ 2.18万
依托单位：
Iwate Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

Bisphenol A is one of the endocrine disturbing materials, which gives its influence mostly to the fetus. It will give a very important information for the safety of the material to investigate its distribution to the whole body, accumulation in the body and excretion to outside of body when incorporated to the body in the time of fetus.Four mice in the thirteenth day of pregnancy were used. ^<14>C-bisphenl A was injected intraperitoneally to the pregnant mice at 4.3 mg BPA/kg body weight (50 uCi/mouse). Each mouse was killed at one hour, one day, three days and five days after the injection. The sacrificed entire mouse body was immediately frozen and approximately 10 urn-thick whole body section was cut out sagittaly along the center of the mouse body using cryomicrotome. Five mice delivered by the fifth day after the injection were processed in the same way. The auto radiographic image of the mice was obtained and analyzed using the imaging plate and fluorescent/radioisotope image analyzer.The ^<14>C-bisphenol A distributed to the whole body including the fetuses one hour after the injection. High concentration of ^<14>C was detected in liver, ileum, submandibular gland) kidney, gall bladder and wall of uterus. The liver in the fetus already showed more concentrated ^<14>C than the other part of fetus body. At 24 hours, the ^<14>C was decreasing in its amount in the whole body except in liver, transverse colon and also in the jejunum right under the peritoneal. At three days, ^<14>C was detected mostly in the abdominal cavity, especially the fetus wall) liver, cortex of kidney, ileum, jejunum and colon were sensitive organs. By fifth day, the radioisotope activity of the whole body was much weaker and the activity was limited to liver, cortex of kidney, intestine and colon. The ^<14>C activity was transmitted to baby mice and detected in the liver area even though its activity was very weak.

双酚A是一种内分泌干扰物质，对胎儿的影响最大。研究该物质在胎儿期进入体内后的全身分布、体内蓄积和体外排泄情况，将为该物质的安全性提供非常重要的信息。以<14>4.3 mg BPA/kg体重（50 uCi/小鼠）向妊娠小鼠腹膜内注射14 C-双酚A。每只小鼠在注射后1小时、1天、3天和5天处死。将处死的整个小鼠身体立即冷冻，并使用冷冻切片机沿小鼠身体中心矢状沿着切下约10 μ m厚的整个身体切片。以相同的方式处理注射后第五天分娩的五只小鼠。用成像板和荧光/放射性同位素图像分析仪获得小鼠的自动X线图像并进行分析，<14>注射后1小时，1C-双酚A分布于包括胎儿在内的全身。在<14>肝脏、回肠、颌下腺、肾脏、胆囊和子宫壁中检测到高浓度的^ C。胎儿的肝脏已经显示出<14>比胎儿身体的其他部分更浓的^ C。在24小时，<14>除肝脏、横结肠和腹膜正下方的空肠外，全身^ C的量均减少。第3天，<14>腹腔内主要检出~（13）C，尤其是胎肝、肾皮质、回肠、空肠和结肠为敏感器官。到第5天，全身放射性同位素活性明显减弱，仅限于肝脏、肾皮质、肠和结肠。尽管^<14>C的活性非常微弱，但它还是被传递给了幼鼠，并在肝脏区域被检测到。