Accumulation of Bisphenol A in the body

双酚A是内分泌干扰物质之一，对胎儿的影响最大。研究该物质在胎儿期进入体内后在全身的分布、在体内的积累以及向体外的排泄，将为该物质的安全性提供非常重要的信息。使用4只怀孕第13天的小鼠。以4.3 mg BPA/kg体重（50 uCi/只小鼠）腹腔注射c -双酚A。分别于注射后1小时、1天、3天和5天处死小鼠。立即将牺牲的整个小鼠身体冷冻，并沿小鼠身体中心矢状切出约10缸厚的全身切片。注射后第5天出生的5只小鼠采用同样的处理方法。利用成像板和荧光/放射性同位素图像分析仪获得小鼠的自动x线图像并进行分析。注射1小时后，c -双酚A分布到包括胎儿在内的全身。肝脏、回肠、颌下腺、肾脏、胆囊和子宫壁均可见高浓度的^<14>C。胎儿肝脏已显示出比胎儿身体其他部位更集中的<14>C。24小时时，除肝脏、横结肠和腹膜正下方空肠外，全身^<14>C含量均下降。第3天，主要在腹腔（尤其是胎壁）检出^<14>C，肝、肾皮质、回肠、空肠和结肠为敏感器官。到第5天，全身放射性同位素活性明显减弱，仅限于肝脏、肾皮质、肠和结肠。^<14>C活性被传递给幼鼠，并在肝脏区域检测到，尽管其活性非常弱。

Bisphenol A is one of the endocrine disturbing materials, which gives its influence mostly to the fetus. It will give a very important information for the safety of the material to investigate its distribution to the whole body, accumulation in the body and excretion to outside of body when incorporated to the body in the time of fetus.Four mice in the thirteenth day of pregnancy were used. ^<14>C-bisphenl A was injected intraperitoneally to the pregnant mice at 4.3 mg BPA/kg body weight (50 uCi/mouse). Each mouse was killed at one hour, one day, three days and five days after the injection. The sacrificed entire mouse body was immediately frozen and approximately 10 urn-thick whole body section was cut out sagittaly along the center of the mouse body using cryomicrotome. Five mice delivered by the fifth day after the injection were processed in the same way. The auto radiographic image of the mice was obtained and analyzed using the imaging plate and fluorescent/radioisotope image analyzer.The ^<14>C-bisphenol A distributed to the whole body including the fetuses one hour after the injection. High concentration of ^<14>C was detected in liver, ileum, submandibular gland) kidney, gall bladder and wall of uterus. The liver in the fetus already showed more concentrated ^<14>C than the other part of fetus body. At 24 hours, the ^<14>C was decreasing in its amount in the whole body except in liver, transverse colon and also in the jejunum right under the peritoneal. At three days, ^<14>C was detected mostly in the abdominal cavity, especially the fetus wall) liver, cortex of kidney, ileum, jejunum and colon were sensitive organs. By fifth day, the radioisotope activity of the whole body was much weaker and the activity was limited to liver, cortex of kidney, intestine and colon. The ^<14>C activity was transmitted to baby mice and detected in the liver area even though its activity was very weak.