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PRECISE ANALYSIS AND DISSOLVING BEHAVIOR OF BISPHENOL A (ENDOCRINE DISRUPTING CHEMICAL) IN DENTAL MATERIALS.

牙科材料中双酚 A（内分泌干扰化学品）的精确分析和溶解行为。

基本信息

批准号：
10671928
负责人：
TANAKA Mitsuro
金额：
$ 1.98万
依托单位：
Tokyo Medical and Dental University, Faculty of Dentistry
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

The pit and fissure sealant is used very frequently in the pediatric dentistry clinic. It is possible that bisphenol A, one of the endocrine disturbing chemicals, might be contained in the sealant. The concentration of the bisphenol A in the sealant was measured in this study.In the first year, analysis of estrogen and bisphenol A was conducted using a yeast-based steroid hormone receptor gene transcription assay. A linear dose response relation was obtained between 10ィイD1-8ィエD1M to 10ィイD1-7ィエD1M for estrogen and 10ィイD1-4ィエD1M to 10ィイD1-3ィエD1M for bisphenol A. Higher or lower concentration that these ranges resulted in a lower estrogenic reaction. These data indicated that bisphenol A had 10ィイD1-4ィエD1 of reactivity than estrogen. Because of the poisonous nature of the sealant itself, about 20 times of dilution was necessary to apply sealant to the assay. This concentration did not cause any estrogenic reaction. In the second year, GCMS method was used in order to analyze much smaller amount of bisphenol A. As only very small amount of bisphenol A exists in sealant, a concentration and purification procedure was conducted before the application of the sample. Two times of extraction of bisphenol A were conducted with 30 ml of methanol against 2.5185 g of sealant. The extract was applied to silicagel chromatography containing 5 % water for the purification. This purified sample was applied to the GCMS and finally 0.025μg of bisphenol A was detected per 1 g of sealant.

窝沟封闭剂是儿科临床常用的封闭剂。密封剂中可能含有内分泌干扰物之一的双酚A。本研究检测了密封剂中双酚A的含量。第一年，采用酵母类固醇激素受体基因转录分析方法对雌激素和双酚A进行了分析。雌激素在10ィイd1-8ィエD1M至10ィイd1-7ィエD1M和双酚A在10ィイd1-4ィエD1M至10ィイd1-3ィエD1M之间呈线性剂量反应关系。这些数据表明，双酚A的反应性比雌激素高10ィイd1-4ィエd1。由于密封剂本身的毒性，将密封剂应用于检测需要大约20倍的稀释。该浓度未引起任何雌激素反应。第二年，由于密封胶中只有极少量的双酚A存在，采用气相色谱-质谱法分析双酚A的含量较少，在样品使用前进行了浓缩和纯化程序。用30毫升甲醇对2.5185克密封胶进行两次提取双酚A。提取液用含5%水的硅胶层析法进行纯化。将纯化的样品应用于气相色谱-质谱仪，每1 g密封胶中检测到0.025μg的双酚A。