Effects of mechanical force on RANKL expression and osteoclasts formation in PDL cells
机械力对 PDL 细胞 RANKL 表达和破骨细胞形成的影响
基本信息
- 批准号:14571968
- 负责人:
- 金额:$ 2.18万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2002
- 资助国家:日本
- 起止时间:2002 至 2003
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We found in the present study that not tensile force but compressive force applied to the periodontal ligament (PDL) cells induces RANKL mRNA. It may be related to the osteoclasts genesis in the compression side during orthodontic tooth movement. As expression of RANKL is usually little, it is so difficult to detect RANKL expression clearly in any experiments. The expression of cox-2 in the PDL cells, the most important enzyme for PGE_2 synthesis and responsible for osteoclasts genesis, is significantly stimulated in response to both compressive and tensile forces, and the intensity of expression is much dependent on the donor age. These data may be involved in the fact that gingival recession and alveolar crest resorption during orthodontic treatment is more frequent in the adult patients than in the young patients. Even through the cox-2 expression of PDL cells in response to mechanical force is high, RANKL expression is unclear or very low. It is known that PGE_2 has been shown to stimulate RANKL expression, but RANKL is mediated not only expression of cox-2 but also other factors. Another experiments show that the certain magnitude of compressive force to the osteoblats (Saos-2) stimulates BSP expression via PGE_2 synthesis, and cox-2 may have important roles of bone formation. The experiments to clarify anti-inflammatory effects of low-energy laser irradiation show that low-energy laser irradiation dramatically inhibits cox-2 expression from PDL cells in response to cyclic tension force. There is high possibility that low-energy laser irradiation to the moved teeth may reduce alveolar crest resorption during orthodontic treatment. Further studies such as effects of different laser dose, frequency and wavelength to PDL cells on bone resorption factors may be necessary for clinical use.
我们在本研究中发现,施加于牙周膜 (PDL) 细胞的压力而非拉力会诱导 RANKL mRNA。这可能与正畸牙齿移动时受压侧破骨细胞的生成有关。由于RANKL的表达通常很少,因此在任何实验中都很难清楚地检测到RANKL的表达。 PDL 细胞中 cox-2 的表达是 PGE_2 合成中最重要的酶,负责破骨细胞的生成,在压缩力和拉力的作用下会显着刺激,并且表达强度很大程度上取决于供体年龄。这些数据可能与正畸治疗期间牙龈退缩和牙槽嵴吸收在成年患者中比年轻患者更常见有关。即使通过响应机械力的PDL细胞的cox-2表达很高,RANKL表达也不清楚或很低。已知PGE_2已被证明可以刺激RANKL表达,但RANKL不仅介导cox-2的表达,还介导其他因子的表达。另一项实验表明,对成骨细胞(Saos-2)施加一定程度的压缩力可通过PGE_2的合成刺激BSP的表达,并且cox-2可能在骨形成中发挥重要作用。阐明低能激光照射的抗炎作用的实验表明,低能激光照射显着抑制 PDL 细胞响应循环张力的 cox-2 表达。在正畸治疗期间,对移动的牙齿进行低能量激光照射很可能会减少牙槽嵴吸收。临床应用可能需要进一步研究不同激光剂量、频率和波长对PDL细胞对骨吸收因子的影响。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
SHIMIZU Noriyoshi其他文献
SHIMIZU Noriyoshi的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('SHIMIZU Noriyoshi', 18)}}的其他基金
The effect of low-power laser irradiation on DFAT differentiation
低功率激光照射对DFAT分化的影响
- 批准号:
26463101 - 财政年份:2014
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The elucidation of sharing roles of periodontal ligament cells and alveolar bone osteoblasts in orthodontic-force responsive osteoclast formation.
阐明牙周膜细胞和牙槽骨成骨细胞在正畸力响应破骨细胞形成中的共同作用。
- 批准号:
22592298 - 财政年份:2010
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Improvement of the mini-screw stability for orthodontic treatmentby laser irradiation
激光照射正畸治疗微型螺钉稳定性的提高
- 批准号:
19592369 - 财政年份:2007
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Bone formation stimulation by semiconductor laser via Cbfa1/Pebp2αA
半导体激光通过 Cbfa1/Pebp2αA 刺激骨形成
- 批准号:
12672018 - 财政年份:2000
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
相似海外基金
Foundation construction of nanoactuator technology that interferes with biological systems through mechanical force
通过机械力干扰生物系统的纳米执行器技术的基础构建
- 批准号:
23KJ1862 - 财政年份:2023
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for JSPS Fellows
Regulation of Cell Adhesions by Mechanical Force
机械力对细胞粘附的调节
- 批准号:
2044394 - 财政年份:2021
- 资助金额:
$ 2.18万 - 项目类别:
Standard Grant
Regulation of cell division by mechanical force in tissues: speed versus strength
组织中机械力对细胞分裂的调节:速度与强度
- 批准号:
2627308 - 财政年份:2021
- 资助金额:
$ 2.18万 - 项目类别:
Studentship
The role of notochord derived signaling, mechanical force generation and AF derived Tgfβ signaling on intervertebral disc formation
脊索衍生信号、机械力产生和 AF 衍生 Tgfβ 信号对椎间盘形成的作用
- 批准号:
10414098 - 财政年份:2020
- 资助金额:
$ 2.18万 - 项目类别:
The role of notochord derived signaling, mechanical force generation and AF derived Tgfβ signaling on intervertebral disc formation
脊索衍生信号、机械力产生和 AF 衍生 Tgfβ 信号对椎间盘形成的作用
- 批准号:
10264801 - 财政年份:2020
- 资助金额:
$ 2.18万 - 项目类别:
The roles of mechanical force in cerebral development: focus on the dynamics and fate of neural progenitor cells and their molecular mechanism
机械力在大脑发育中的作用:关注神经祖细胞的动态和命运及其分子机制
- 批准号:
19K06920 - 财政年份:2019
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Fabrication of rotaxane-type meso-macroscopic molecular devices whose molecular motions can be converted into mechanical force
分子运动可转化为机械力的轮烷型介观分子器件的制备
- 批准号:
19H02787 - 财政年份:2019
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
The relationship between the mechanical force and the interaction of skin-immune system during wound healing process
伤口愈合过程中机械力与皮肤-免疫系统相互作用的关系
- 批准号:
16K15755 - 财政年份:2016
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Measurement and functional analysis of left-right asymmetric mechanical force driving left-right asymmetric morphogenesis in Drosophila
果蝇左右不对称机械力驱动左右不对称形态发生的测量和功能分析
- 批准号:
15H04378 - 财政年份:2015
- 资助金额:
$ 2.18万 - 项目类别:
Grant-in-Aid for Scientific Research (B)