Study on dynamics and physiology of antibiotic resistant bacteria in natural environments

自然环境中抗生素耐药菌的动态和生理学研究

• 批准号: 14572109
• 负责人: TANI Katsuji
• 金额: $ 2.18万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14572109/
• 关键词: tetracycline; β-lactcm; river; surface water; biofilm; bacteria; antibiotic resistance; PCR; テトラサイクリン耐性遺伝子; βラクタマーゼ遺伝子; マイクロコズム; 系統解析; 抗生物質耐性; 汚染; 多様性

Understanding of dynamics of antibiotic resistant bacteria in natural environments contributes to prevention, of their spread. It is important to consider not only resistant bacteria but also resistance genes, because the genes are able to move between different bacteria. In this study distribution of the antibiotic resistance genes in the rivers and bacterial responses to antibiotic were investigated.1. Diversity of tetracycline resistance genes in the river water was higher in the polluted site than in the unpolluted sites.2. The PCR primer sets for amplifying the beta-lactamase genes were designed. Various beta-lactamase genes could be detected in the river water by PCR with newly designed primers.3. Dynamics of tetracycline resistant bacteria was investigated using a microcosm which consisted of river water and glass beads for biofilm formation. Diversity was higher in biofilm forming bacteria than in free living bacteria., Bacteria could acquire antibiotic resistance and survive in a biofilm.because of transfer of antibiotic genes and change of permeability of antibiotics intothe cells.

了解自然环境中抗生素耐药性细菌的动态有助于预防其传播。重要的是不仅要考虑耐药细菌，还要考虑耐药基因，因为这些基因能够在不同的细菌之间移动。本研究调查了河流中抗生素耐药基因的分布和细菌对抗生素的反应.污染区河水中四环素抗性基因的多样性高于未污染区.设计了β-内酰胺酶基因PCR扩增引物。用新设计的引物进行PCR检测，可在河水中检出多种β-内酰胺酶基因.以河水和玻璃微珠组成的微宇宙为载体，研究了四环素耐药菌的动态变化。生物膜形成细菌的多样性高于自由生活细菌，由于抗生素基因的转移和抗生素对细胞通透性的改变，细菌可以获得抗生素耐药性并在生物被膜中存活。