Study on dynamics and physiology of antibiotic resistant bacteria in natural environments

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Understanding of dynamics of antibiotic resistant bacteria in natural environments contributes to prevention, of their spread. It is important to consider not only resistant bacteria but also resistance genes, because the genes are able to move between different bacteria. In this study distribution of the antibiotic resistance genes in the rivers and bacterial responses to antibiotic were investigated.1. Diversity of tetracycline resistance genes in the river water was higher in the polluted site than in the unpolluted sites.2. The PCR primer sets for amplifying the beta-lactamase genes were designed. Various beta-lactamase genes could be detected in the river water by PCR with newly designed primers.3. Dynamics of tetracycline resistant bacteria was investigated using a microcosm which consisted of river water and glass beads for biofilm formation. Diversity was higher in biofilm forming bacteria than in free living bacteria., Bacteria could acquire antibiotic resistance and survive in a biofilm.because of transfer of antibiotic genes and change of permeability of antibiotics intothe cells.