STUDY OF GRANULYSIN AS A USEFUL PARAMETER FOR ASSESSING PATHOLOGICAL STATUS

颗粒溶素作为评估病理状态有用参数的研究

• 批准号: 14572196
• 负责人: KISHI Atsuko
• 金额: $ 1.98万
• 依托单位: LOUIS PASTEUR CENTER FOR MEDICAL RESEARCH
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14572196/
• 关键词: GRANULYSIN; NK CELL; CD8T CELL; CYTOLYTIC GRANULE; PATHOLOGICAL CONDITION; granulysin; perforin; 細胞障害性顆粒; 癌; 免疫機能

We set up a system using mAbs of detecting granulysin using ELISA method and revealed that granulysin can be detected from the supernatants of PBMC without any stimulation. Ganulysin production of healthy individuals is correlated with the number of NK cells, whereas some cancer patients do not show any correlation with NK cells due to the high production from CD8+ T cells.

我们使用使用ELISA方法检测颗粒素的mAb建立了一个系统，并发现可以从PBMC的上清液中检测到颗粒素，而无需任何刺激。健康个体的Ganulysin产生与NK细胞的数量相关，而由于CD8+ T细胞产生的高产生，一些癌症患者与NK细胞没有任何相关性。

项目成果

Kishi A, Takamori Y, Ogawa K, Takano S, Tomita S, Tanigawa M, Niman M, Kishida T, Fujita S: "Differential expression of granulysin and perforin by NK cells in cancer patients and correlation of impaired granulysin expression with progression of cancer"Can

Kishi A、Takamori Y、Okawa K、Takano S、Tomita S、Tanikawa M、Niman M、Kishida T、Fujita S：“癌症患者中 NK 细胞颗粒溶素和穿孔素的差异表达以及颗粒溶素表达受损与癌症进展的相关性