Establishment of the screening system of vaccine candidate genes against cysticercosis using Nod-scid mice.

囊虫病是人类最严重的幼虫绦虫病之一，在医学上具有重要意义，在猪中具有重要的经济意义，目前正在世界范围内蔓延，特别是在亚洲、非洲和拉丁美洲。猪带绦虫囊尾蚴病疫苗的研制在许多国家进行了试验，实际上也报道了一些候选分子。然而，使用这些分子的疫苗效果并不总是完美的。表达文库免疫已被提出作为一种有效的手段来筛选大量的病原体的基因作为候选保护分子。在先前的研究中，我们使用带绦虫-大鼠模型系统证明了表达文库免疫用于抗猪囊尾蚴病疫苗开发的功效。为了挑选出疫苗候选基因，我们必须检查许多克隆。然而，猪是T.索利姆不容易用于这种筛选试验。本研究的目的是建立Nod-Scid小鼠的筛选体系，虽然我们在前期的研究中已经建立了成虫cDNA文库，但文库的质量不足以用于文库疫苗的筛选。因此，我们利用5 '端和3'端race系统从六钩蚴或卵中构建了一个新的cDNA文库。新cDNA文库的质量足以满足疫苗的使用要求。

Cysticercosis, one of the most serious larval cestodiasis of medical importance in humans and of economic importance in pigs, is spreading worldwide, especially in Asia, Africa and Latin America. The trial of the vaccine development against Taenia solium cysticercosis has been made in many countries, and actually some candidate molecules have been also reported. However, the effects of vaccine used those molecules have been not always perfect. Expression-library immunization has been proposed as an effective means to screen a large number of genes of the pathogen as candidate protective molecules. In the previous study, we demonstrated the efficacy of expression-library immunization for vaccine development against T solium cysticercosis using T taeniaeformis- rat model system. In order to pick out the vaccine candidate genes, we have to check many clones. However, pigs, which are intermediate hosts of T. solim, are not easy to use for such screening test. Then the purpose of this study is to establish the screening system using Nod-Scid mouse.Although we have created the cDNA library from adult worms in the previous study, qualoity of the cDNA library was insufficient for using the library vaccine. So we made a new cDNA library from oncospheres or eggs using the 5'and 3'race system. The quarity of the new cDNA library seemd to be sufficient for using the vaccine.