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Establishment of the screening system of vaccine candidate genes against cysticercosis using Nod-scid mice.

Nod-scid小鼠囊尾蚴病疫苗候选基因筛选体系的建立

基本信息

批准号：
16500277
负责人：
OKAMOTO Munehiro
金额：
$ 1.98万
依托单位：
Tottori University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16500277/
关键词：
Taenia taeniaerormis Library Vaccine Protective Antigen immunity Taeniid Cestode Nod-Scid mouse rat テニア科条虫 Nod-Scid

项目摘要

Cysticercosis, one of the most serious larval cestodiasis of medical importance in humans and of economic importance in pigs, is spreading worldwide, especially in Asia, Africa and Latin America. The trial of the vaccine development against Taenia solium cysticercosis has been made in many countries, and actually some candidate molecules have been also reported. However, the effects of vaccine used those molecules have been not always perfect. Expression-library immunization has been proposed as an effective means to screen a large number of genes of the pathogen as candidate protective molecules. In the previous study, we demonstrated the efficacy of expression-library immunization for vaccine development against T solium cysticercosis using T taeniaeformis- rat model system. In order to pick out the vaccine candidate genes, we have to check many clones. However, pigs, which are intermediate hosts of T. solim, are not easy to use for such screening test. Then the purpose of this study is to establish the screening system using Nod-Scid mouse.Although we have created the cDNA library from adult worms in the previous study, qualoity of the cDNA library was insufficient for using the library vaccine. So we made a new cDNA library from oncospheres or eggs using the 5'and 3'race system. The quarity of the new cDNA library seemd to be sufficient for using the vaccine.

囊虫病是对人类具有重要医学意义和对猪具有重要经济意义的最严重的幼虫囊虫病之一，正在世界范围内蔓延，特别是在亚洲、非洲和拉丁美洲。许多国家都进行了猪带绦虫囊虫病疫苗研制的试验，实际上也报道了一些候选分子。然而，使用这些分子的疫苗的效果并不总是完美的。表达文库免疫被认为是一种筛选大量病原体基因作为候选保护分子的有效手段。在之前的研究中，我们利用带绦虫-大鼠模型系统证明了表达文库免疫对猪囊尾蚴病疫苗开发的有效性。为了挑选出疫苗候选基因，我们必须检查许多克隆。然而，猪是梭状螺旋体的中间宿主，不容易用于这种筛选试验。本研究的目的是建立Nod-Scid小鼠筛选系统。虽然我们在之前的研究中建立了成虫的cDNA文库，但cDNA文库的质量不足以用于文库疫苗的使用。因此，我们利用5′和3′小种系统从癌球或卵中构建了新的cDNA文库。新cDNA文库的质量似乎足以用于疫苗的使用。