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In vivo chondrogenesis by applying composites of synovium-derived mesenchymal stem cells with collagen gel covered with periosteum.

通过应用滑膜来源的间充质干细胞与骨膜覆盖的胶原凝胶的复合物进行体内软骨形成。

基本信息

批准号：
16500287
负责人：
ICHINOSE Shizuko
金额：
$ 2.43万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

Mesenchymal stem cells (MSCs) have the potential to differentiate into the lineages of mesenchymal tissues. The aim of this study is to clarify the detailed process of cartilage regeneration in MSC_s.We examined "In vivo chondrogenesis by applying composites of synovium-derived mesenchymal stem cells with collagen gel covered with periosteum" as follows.(1) Comparison of stem cells derived from bone marrow, synovium and cartilage tissue.We demonstrated that synovium-derived MSCs had greater in vitro chondrogenic ability, suggesting an optimal cell source for cartilage regeneration.(2) In vitro cartilage formation of composites of MSCs with collagen gel.We demonstrated that the composites of synovium-derived MSCs with collagen gel had greater in vitro chondrogenic and osteogenic ability.(3) Transplantation.We transplanted composites of MSCs with collagen gel into a full-thickness articular cartilage defect of adult rabbits. After 4 weeks, although the cell density decreased, transplanted MSCs produced a great amount of cartilage matrix extensively. The periosteum became thinner and chondroprogenitors in the periosteum produced a small amount of cartilage matrix. In the deeper zone, transplanted MSCs progressed to the hypertrophic chondrocytes. In the deep zone, some transplanted cells differentiated into bone cells and cells around medullary cavity were replaced with host cells. In the next phase, border between bone and cartilage progressed to the original height. Also, integrations between native and regenerated cartilage were improved. Transplanted synovium-derived MSCs altered over a time course according to the micro environments. Our results will advance MSC-based therapeutic strategies for cartilage injury and provide the clues for the mechanisms that govern multilineage differentiation of MSCs.

间充质干细胞（MSCs）具有分化为间充质组织谱系的潜力。本研究的目的是阐明MSC_s软骨再生的详细过程。我们研究了“利用滑膜来源的间充质干细胞与骨膜覆盖的胶原凝胶的复合材料进行体内软骨形成”，结果如下。(1)骨髓、滑膜和软骨组织干细胞的比较。我们证明了滑膜来源的MSCs具有更大的体外软骨形成能力，这表明它是软骨再生的最佳细胞来源。(2) MSCs与胶原凝胶复合材料的体外软骨形成。我们证明了滑膜来源的MSCs与胶原凝胶的复合材料具有更大的体外成软骨和成骨能力。(3)移植。我们将MSCs与胶原凝胶复合移植到成年兔全层关节软骨缺损中。4周后，移植的MSCs虽然细胞密度下降，但大量产生软骨基质。骨膜变薄，骨膜内的软骨祖细胞产生少量软骨基质。在深部区，移植的间充质干细胞向增生性软骨细胞发展。在深部区，部分移植细胞分化为骨细胞，髓腔周围细胞被宿主细胞取代。在下一阶段，骨和软骨之间的边界发展到原来的高度。此外，原生软骨和再生软骨之间的整合也得到了改善。移植的滑膜来源的间充质干细胞在一段时间内根据微环境发生改变。我们的研究结果将推进基于间充质干细胞的软骨损伤治疗策略，并为控制间充质干细胞多谱系分化的机制提供线索。

项目成果

期刊论文数量（40）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

In vitro cartilage formation of composites of synovium-derived mesenchymal stem cells with collagen gel

DOI：
10.1007/s00441-005-0010-6
发表时间：
2005-11-01
期刊：
CELL AND TISSUE RESEARCH
影响因子：
3.6
作者：
Yokoyama, A;Sekiya, I;Muneta, T
通讯作者：
Muneta, T

Amlodipine and carvedilol prevent cytotoxicity in cortical neurons isolated from stroke-prone spontaneously hypertensive rats.

DOI：
10.1291/hypres.27.271
发表时间：
2004-04
期刊：
Hypertension research : official journal of the Japanese Society of Hypertension
影响因子：
0
作者：
K. Yamagata;S. Ichinose;M. Tagami
通讯作者：
K. Yamagata;S. Ichinose;M. Tagami

Morphological examination during in vitro cartilage formation by human mesenchymal stem cells