Study on the Distribution Behavior and Toxicity of Metallic Wear Particles and Ion Released from Total Knee Prosthesis.

全膝关节假体释放金属磨损颗粒和离子的分布行为及毒性研究。

• 批准号: 10680791
• 负责人: ICHINOSE Shizuko
• 金额: $ 0.32万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10680791/
• 关键词: Biomaterial; Total knee prosthesis; Toxicity; Co-Cr-Mo alloy; Ti-Al-V alloy; Wear particle; Dissolution; Electron microscopic observation; 毒性の発現; 合金微粒子とイオン化; マクロファージ; Co-Cr合金

We surgically obtained two Co-Cr-Mo and five Ti-Al-V alloy knee prosthesis which were retrieved due to mechanical failure and pain. We examined the distribution and the size of the small particles which were released from Co-Cr-Mo and Ti-Al-V alloy using backscattered SEM and TEM. In addition we analyzed the metal ions in the adjacent tissues of the knee prosthesis by energy dispersive X-ray spectroscopy(EDS).We observed that small particles were detected evenly in the synovial tissues and the particles contained Co-Cr-Mo and Ti-Al-V alloy components. Metallic wear particles were phagocyted by the cells next to the synovial bursae and showed bulky or needle-like structures. We demonstrated that both Co-Cr-Mo particles and Cr particles were present in the cells adjacent to Co-Cr-Mo alloy, although Ti-Al-V particles alone were found in cases using Ti-Al-V alloy prosthesis. These results suggest that Co ion released from Co-Cr-Mo alloy enters the surrounding tissues as well as blood stream and damages the body.

我们通过手术获得了两个钴铬钼和五个钛铝钒合金膝关节假体，由于机械故障和疼痛而被取出。利用背散射扫描电镜和透射电镜研究了Co-Cr-Mo和Ti-Al-V合金中析出的小颗粒的分布和尺寸。此外，我们还利用X射线能谱仪（EDS）对膝关节假体周围组织中的金属离子进行了分析，观察到滑膜组织中均匀分布着细小的金属颗粒，这些金属颗粒中含有Co-Cr-Mo和Ti-Al-V合金成分。金属磨损颗粒被滑膜囊附近的细胞吞噬，并显示出粗大或针状结构。我们证明，Co-Cr-Mo颗粒和Cr颗粒都存在于Co-Cr-Mo合金附近的细胞中，尽管在使用Ti-Al-V合金假体的病例中仅发现Ti-Al-V颗粒。这些结果表明，钴铬钼合金释放的钴离子进入周围组织，以及血液和损害身体。

项目成果

Ichinose S. Muneta T, Miyazaki K and Ojima M: "Study on the distribution and toxicity of HDP and metallic wear particles induced by total knee prosthesis - Observation by TEM -"Jpn. J. Clin. Electron Microsc.. Vol. 32 Supplement. S136 (1999)

Ichinose S. Muneta T、Miyazaki K 和 Ojima M：“全膝关节假体引起的 HDP 和金属磨损颗粒的分布和毒性研究 - TEM 观察 -”Jpn。

市野瀬志津子: "整形外科用人工関節から生じるポリエチレン摩耗粉と金属微粒子の生体内分布と毒性の研究-滑膜を中心とした周囲組織の電顕観察-"日本臨床電子顕微鏡学会誌. 32・増刊号. S136 (1999)

Shizuko Ichinose：“骨科人工关节产生的聚乙烯磨损颗粒和细金属颗粒的生物分布和毒性研究 - 滑膜和周围组织的电子显微镜观察 -”日本临床电子显微镜学会杂志，第 32 期。 .S136（1999）

Ito S, Muneta T, Shinomiya K and Ichinose S: "Electron microscopic evaluation of the effects of stress-shielding on maturation of the mid-substance and ligament-bone junction of the reconstructed anterior cruciate ligament in rabbits."Journal of Materials

Ito S、Muneta T、Shinomiya K 和 Ichinose S：“电子显微镜评估应力屏蔽对兔子重建前十字韧带的中间物质和韧带骨连接处成熟的影响。”材料杂志

市野瀬志津子: "軟骨細胞の変性および軟骨気質蛋白の異常に関する免疫電顕的検討"電子顕微鏡. 35・増刊号(印刷中). (2000)

Shizuko Ichinose：“软骨细胞变性和软骨蛋白异常的免疫电子显微镜研究”电子显微镜第 35 期，特刊（2000 年）。

Ichinose S, Muneta T, Miyazaki K, Tagami M and Ojima M: "Immuno-gold electron microscopic evaluation of degeneration of chondral cell and abnormality of chondral matrix protein."Electron microscopy. Vol. 35 Supplement (in print). (2000)

Ichinose S、Muneta T、Miyazaki K、Tagami M 和 Ojima M：“软骨细胞变性和软骨基质蛋白异常的免疫金电子显微镜评估。”电子显微镜。