Optimal exercise intensity for defense ability to oxidative stress

氧化应激防御能力的最佳运动强度

• 批准号: 16500397
• 负责人: NAGASAWA Junichi
• 金额: $ 2.37万
• 依托单位: The University of Electro-Communications
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16500397/
• 关键词: active oxygen; physical exercise; lactate; 生理学; 活性酵素; 運動強度; 酸化ストレス

The purpose of the study was to evaluate the effects of physical exercise to antioxidation power in plasma. Six male subjects (21-25 years old) performed maximal exercise by a gradual increase load using a bicycle ergometer. Exercise load was raised by 25W every four minutes to exhaustion. Blood samples were obtained from the antecubital vein at rest, each load stage, and 10 minutes, 1-hour and 1-day after exercise. As antioxidation power, we measured iron reduction power (Fe^<3+>→Fe^<2+>). The quantity of hydroxyl radical generation was measured by a spin trap method. The lactic acid and the pyruvic acid were measured by an enzyme method. Six another subjects exercised with a physical work as equivalence. The iron reduction power in plasma rose with an increase in exercise intensity. On the contrary, the quantity of generation of a hydroxyl radical decreased with a rise of exercise intensity. This shows that antioxidation power of plasma increases by a rise of exercise intensity. In addition, there were negative relations to lactic acid and ESR signal intensity. These data supported that the plasma lactic acid which increased by exercise has a role of a scavenger of active oxygen.

本研究的目的是评价体育锻炼对血浆抗氧化能力的影响。6名男性受试者(21-25岁)使用自行车测功器进行递增负荷的最大运动。运动负荷每4分钟增加25W至力竭。分别于安静、各负重阶段、运动后10min、1h、1d从肘前静脉取血。作为抗氧化能力，我们测定了铁还原能力(Fe^&lt；3+&gt；→Fe^&lt；2+&gt；)。用自旋捕获法测定了羟基自由基产生量。用酶法测定乳酸和丙酮酸。另有六名受试者以体力劳动为等量进行锻炼。血浆铁还原力随运动强度的增加而升高。相反，羟基自由基的生成量随着运动强度的增加而减少。这表明，随着运动强度的增加，血浆抗氧化能力增强。此外，与血乳酸、ESR信号强度呈负相关。这些数据支持运动增加的血浆乳酸具有清除活性氧的作用。