Optimal exercise intensity for defense ability to oxidative stress

本研究旨在探讨运动对血浆抗氧化能力的影响。六名男性受试者（21-25岁）使用自行车测力计进行逐渐增加负荷的最大运动。运动负荷每4分钟增加25 W至力竭。分别在安静时、各负荷阶段、运动后10分钟、1小时和1天从肘前静脉采集血样。作为抗氧化能力，我们测量了铁还原能力（Fe^<3+>→Fe^<2+>）。羟基自由基产生的量通过自旋捕获法测量。通过酶法测定乳酸和乳酸。另外6名受试者进行体力劳动锻炼作为等效。血浆铁还原能力随运动强度的增加而增加。相反，羟自由基的产生量随着运动强度的增加而减少。这说明血浆的抗氧化能力随运动强度的增加而增强。此外，乳酸与ESR信号强度呈负相关。这些数据支持运动增加的血浆乳酸具有活性氧清除剂的作用。

The purpose of the study was to evaluate the effects of physical exercise to antioxidation power in plasma. Six male subjects (21-25 years old) performed maximal exercise by a gradual increase load using a bicycle ergometer. Exercise load was raised by 25W every four minutes to exhaustion. Blood samples were obtained from the antecubital vein at rest, each load stage, and 10 minutes, 1-hour and 1-day after exercise. As antioxidation power, we measured iron reduction power (Fe^<3+>→Fe^<2+>). The quantity of hydroxyl radical generation was measured by a spin trap method. The lactic acid and the pyruvic acid were measured by an enzyme method. Six another subjects exercised with a physical work as equivalence. The iron reduction power in plasma rose with an increase in exercise intensity. On the contrary, the quantity of generation of a hydroxyl radical decreased with a rise of exercise intensity. This shows that antioxidation power of plasma increases by a rise of exercise intensity. In addition, there were negative relations to lactic acid and ESR signal intensity. These data supported that the plasma lactic acid which increased by exercise has a role of a scavenger of active oxygen.