Comparative analysis of replication timing among human cancer cell lines using microarray

使用微阵列比较分析人类癌细胞系的复制时间

• 批准号: 16510144
• 负责人: WATANABE Yishihisa
• 金额: $ 2.62万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16510144/
• 关键词: replication timing; p53; microarray; genome-wide; genetic instability; chromosome instability; 染色体不安定性; アンプリコン; replicatino timing; S期内複製時期; がん抑制遺伝子p53; ゲノム不安定性; DNAマイクロアレイ

The completion of the human genome sequence will greatly accelerate development of a new branch of biosience and provide fundamental knowledge to biomedical research. We used DNA microarray based on the sequence information to measure replication timing of human genome. Megabase-sized zones that replicate early or late in S phase (thus early/late transition) were defined at the sequence level. Using the newly replicated DNA from cell-cycle fractionated HCT116p53(+/+) and HCT1 116p53(-/-), the replication timing was determined and compared at the genome sequence level. In conclusion, we found that the early/late-switch regions of replication timing coincided with unstable regions of the genome and that genome-wide assessment of replication timing by DNA microarray serve as an efficient strategy for identifying cancer-related genes.

人类基因组测序的完成将大大促进生物科学这一新的分支的发展，并为生物医学研究提供基础知识。我们使用基于序列信息的DNA微阵列来测量人类基因组的复制时间。在序列水平上定义了在S期早期或晚期（因此早期/晚期转换）复制的兆碱基大小的区域。使用来自细胞周期分级分离的HCT 116 p53（+/+）和HCT 1116 p53（-/-）的新复制的DNA，在基因组序列水平上确定并比较复制时间。总之，我们发现，复制时间的早期/晚期转换区域与基因组的不稳定区域相吻合，并且通过DNA微阵列对复制时间进行全基因组评估可以作为识别癌症相关基因的有效策略。

项目成果

Effect of splice-site polymorphisms of theTMPRSS4, NPHP4 andORCTL4 genes on their mRNA expression

• DOI: 10.1007/bf02715838
• 发表时间: 2005-08
• 期刊: Journal of Genetics
• 影响因子: 1.5
• 作者: Hidetaka Yamada;K. Shinmura;T. Tsuneyoshi;H. Sugimura

遺伝子多型

遗传多态性

• 发表时间: 2004
• 期刊: 病理と臨床 臨時増刊 Vol.22
• 作者: 渡辺良久;椙村春彦
• 通讯作者: 椙村春彦

Amplicons on human chromosome 11q are located in the early/late-switch regions of replication timing

• DOI: 10.1016/j.ygeno.2004.08.001
• 发表时间: 2004-11-01
• 期刊: GENOMICS
• 影响因子: 4.4
• 作者: Watanabe, Y;Ikemura, T;Sugimura, H
• 通讯作者: Sugimura, H