Unique Properties of the Interfacial Phase of the Adsorbed Reverse Micelle and Development of a New Chromatographic Separation Method

人们对反胶束的独特性质越来越感兴趣。此外，反胶束已被视为多功能微反应器。反胶束在化学分析中的应用受到了相当大的关注。在这项工作中，表面活性剂十六烷基三甲基氯化铵（CTAC）的反胶束成功地附着在多孔硅胶或玻璃表面。所得的反胶束相将表现出许多与传统胶束介质相关的反胶束性质，此外，ctac -水-二氧化硅界面有望具有独特的物质识别和分离性质，并可用作液相色谱中的固定相。尽管有大量关于反胶束性质的信息，但似乎没有关于在固体载体（如硅胶）上固定反胶束的先前报道。为了获得有助于理解反胶束界面相作用的知识，并开发一种新的利用反胶束固定相的色谱分离方法，本研究重点进行了以下几个方面的研究：(1)石英晶体微天平研究了CTAC反胶束在玻璃表面的吸附以及过氧化氢在吸附后的CTAC胶束的水相中的包封，并将其应用于单线流注射系统反胶束催化的化学发光法测定甲苯中的过氧化氢；(2)反胶束散装溶液中水与表面活性剂的摩尔比对CTAC和水在不同孔径多孔硅胶上反胶束吸附的影响；(3)在硅胶柱上固定化CTAC反胶束，制备反胶束固定相，表征其独特的色谱性能。

There has been increased interest in the unique properties of reverse micelles. Further, reverse micelles have been viewed as versatile microreactors. Use of reverse micelles in chemical analysis has received considerable attention. In this work, reverse micelles of the surfactant cetyltrimethylammonium chloride (CTAC) were successfully attached to porous silica gels or a glass surface. The resultant reversed admicellar phase will exhibit many of the reversed micellar properties associated with the conventional micellar media and in addition the CTAC-water-silica interface can be expected to bear unique properties of material recognition and separation and to be used as a stationary phase in liquid chromatography. There appears to be no prior report concerning the immobilization of reverse micelles on solid supports such as silica gel, despite a considerable amount of information about the properties of the reverse micelles.In order to obtain the knowledge which contributes to our understanding of the role of the interfacial phase of the reversed admicelle and to develop a new chromatographic separation method using the reversed admicellar stationary phase, this research focused on the followings : (1)The quartz crystal microbalance study on the adsorption of the CTAC reverse micelle on a glass surface as well as the entrapping of the hydrogen peroxide in the aqueous phase of the adsorbed CTAC micelle, and its application to the reversed micellar mediated chemiluminescence determination of hydrogen peroxide in toluene using a single line flow injection system ; (2)the effect of the molar ratio of water to surfactant in the reversed micellar bulk solution on the reversed micellar adsorption of CTAC and water onto porous silica gels of various pore sizes ; (3)the preparation, characterization and unique chromatographic performance of the reversed admicellar stationary phase by immobilizing the CTAC reversed micelles on silica gel in a commercial HPLC column.