Peptide adsorption on metal oxide surfaces. Investigating the biomaterial/biological interface with synchrotron radiation.

金属氧化物表面上的肽吸附。

• 批准号: EP/V002341/1
• 负责人: Andrew THOMAS
• 金额: $ 4.59万
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FV002341%2F1
• 关键词: Peptide; adsorption; metal; oxide; surfaces.

The interaction between biological molecules and inorganic material surfaces is of importance in a number of areas from biomedical devices for implantation, sensors and bioreactors. The required behaviours for each of these applications are slightly different, for example in biomedical implants it is thought that a protein conditioning layer is formed at the surface, prior to cell attachment and growth of new tissue. On the other hand, in pharmaceutical applications where protein therapeutics may be manufactured, interactions with the reactor material surfaces can lead to denaturation or agglomeration of the proteins. This may have the effect of removing the desired action protein, or in the worse case, causing unwanted side effects such as protein aggregation.Studies of molecular adsorption on well-characterised surfaces using synchrotron techniques have led to an improved understanding of protein-metal interactions. However, in most cases the surface of a metal implant or pipeline will be terminated with a native oxide layer. In addition, these studies are often carried out under vacuum conditions, thus the effect of water on the adsorption process is not well understood. Obviously in a real biomedical device or pharmaceutical plant, water will be a major component of the surrounding media. Recent advances in photoelectron spectroscopy now allow measurements to be made in the presence of water and water vapour. Photoelectron spectroscopy is an extremely powerful probe of surface chemistry and the adsorption mechanism of molecules, since it is capable of studying just the top 1 - 10 nm of a material surface.In this proposal we will study the adsorption processes of five different amino acids which exhibit varying degrees of acidity/basicity and two small peptides on idealised titanium dioxide surfaces. Rutile titanium dioxide is the native oxide formed on titanium which is widely used both as a metal and alloy in biomedical implants, and is believed to be a key factor in the success of these devices.

生物分子和无机材料表面之间的相互作用在从用于植入的生物医学装置、传感器和生物反应器的许多领域中是重要的。这些应用中的每一个所需的行为略有不同，例如在生物医学植入物中，人们认为在细胞附着和新组织生长之前，在表面形成蛋白质调节层。另一方面，在可制造蛋白质治疗剂的药物应用中，与反应器材料表面的相互作用可导致蛋白质的变性或附聚。这可能会去除所需的作用蛋白质的效果，或者在更坏的情况下，引起不必要的副作用，如蛋白质aggregation.Studies分子吸附在良好的表征表面使用同步加速器技术已经导致了一个更好的理解蛋白质-金属相互作用。然而，在大多数情况下，金属植入物或管道的表面将以天然氧化物层终止。此外，这些研究通常是在真空条件下进行的，因此水对吸附过程的影响还没有得到很好的理解。显然，在真实的生物医学装置或制药厂中，水将是周围介质的主要成分。光电子能谱的最新进展现在允许在水和水蒸气存在的情况下进行测量。光电子能谱是表面化学和分子吸附机制的一个非常强大的探针，因为它能够研究材料表面的顶部1 - 10 nm。在这个提议中，我们将研究五种不同的氨基酸，表现出不同程度的酸碱性和两种小肽在理想化的二氧化钛表面上的吸附过程。金红石二氧化钛是在钛上形成的天然氧化物，钛被广泛用作生物医学植入物中的金属和合金，并且被认为是这些装置成功的关键因素。