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Analyses of acidophilic bacterial communities and environmental changes using microcosms

使用微观世界分析嗜酸细菌群落和环境变化

基本信息

批准号：
16570008
负责人：
SHIKANO Shuichi
金额：
$ 2.18万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16570008/
关键词：
acidophilic bacteria community microcosm acidic lake Lake Katanuma stratification and circulation hydrogen sulfide FISH method DGGE method

项目摘要

1.The Lake Katanuma, a strongly acid lake, was surveyed during April 2004 to December 2006. The thermal pattern in the lake water showed dimictic. During the stratification period from April to August, oxic water with no hydrogen sulfide was detected in the epilimnion, whereas oxygen depletion and accumulated hydrogen sulfide were prominent in the hypolimnion. Oxic conditions and no hydrogen sulfide were observed in the entire water column during the autumn circulation period.2.Two genus specific oligonucleotide probes were used with fluorescent in situ hybridization (FISH) to measure the biomasses of the genera Acidiphilium and Acidithiobacillus, respectively. The genus Acidiphilium dominated the bacteria community at the stratification period, while both genera dominated at the circulation period.3.We made the microcosms from the lake water of the stratification and circulation periods, respectively. These microcosms were cultured under the following conditions ; 1)dark condition, 2) … More low temperature, 3)low dissolved oxygen, 4)ferrous addition, 5)glucose addition, 6)thiosulfate addition, and 7)control. FISH analysis indicated that the increased abundant of Acidithiobacillus and the decrease of Acidiphilium were observed after 1 week in the microcosms treated with the thiosulfate addition and low temperature. In the microcosms of the other conditions, the biomasses of two genera have not changed.4.Denaturing gradient gel electrophoresis (DGGE) analysis of the microbial community in the microcosms revealed that the genus Acidithiobacillus was consisting of two species, At.caldus and At.albertensis and the genus Acidiphilium was mainly composed of A.acidophilum. Other bacteria genera were not detected by DGGE. Although Acidiphilium species are considered the major representatives of the acidic environments such as the Lake Katanuma, the genus Acidithiobacillus could proliferate under the conditions of sulfide supply or low water temperature during autumn circulation period. Less

1.2004年4月至2006年12月对片沼湖进行了调查，该湖是一个强酸性湖泊。湖水中的热模式显示出一水硬铝石。在4~8月的层结期间，表层没有检测到不含硫化氢的氧化水，而表层则以耗氧和积累硫化氢为主。2.利用两个属特异性寡核苷酸探针进行荧光原位杂交(FISH)，分别对嗜酸杆菌属和硫杆菌属的生物量进行了测定。在层化期细菌群落以嗜酸杆菌属为主，在循环期细菌群落以嗜酸杆菌属为主。3.分别从层化期和循环期的湖水中制作微观世界。这些微宇宙在以下条件下培养：1)黑暗条件，2)…更低的温度，3)低溶解氧，4)铁的添加，5)葡萄糖的添加，6)硫代硫酸盐的添加，以及7)控制。FISH分析表明，在加入硫代硫酸盐和低温处理的微宇宙中，1周后，酸性硫杆菌数量增加，嗜酸菌减少。微宇宙中微生物群落的变性梯度凝胶电泳(DGGE)分析表明，酸杆菌属由两个种组成，分别为At.caldus和At.albertensis，而Acidiphilium则主要由嗜酸曲霉组成。DGGE未检出其他细菌属。尽管嗜酸杆菌被认为是片沼湖等酸性环境的主要代表，但在秋季循环期硫化物供应或水温较低的条件下，嗜酸杆菌属可以繁殖。较少