A study on identification of pre-and postsynaptic neural elements of the circadian pacemaker neurons in insects.

昆虫昼夜节律起搏神经元突触前和突触后神经元鉴定的研究。

• 批准号: 16570069
• 负责人: YASUYAMA Kouji
• 金额: $ 2.43万
• 依托单位: Kawasaki Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16570069/
• 关键词: Drosophila melanogaster; Protophormia terraenovae; circadian clock; extraretinal photoreceptors; pigment dispersing factor; synapses; immuno-electron microscopy; three-dimensional reconstruction; 概日時計; 側方ニューロン; 副視髄; 前大脳背側部; 免疫組織化学; 色素拡散因子; 前

To unravel the wiring of the circadian pacemaker networks underlying rhythmic locomotor activity, we surveyed the pre-and post-synaptic neural elements of the circadian pacemaker neurons, using Drosophila melanogaster and Protophormia terraenovae as model organisms. P. terraenovae has PDF (pigment dispersing factor)-immunopositive neurons (called here Pt-PDF/LNs), corresponding to the Drosophila PDF-immunopositive s-LNvs (small ventral lateral neurons) that function as circadian pacemakers. Therefore, P. terraenovae is also a good model species, paralleling D. melanogaster.In D. melanogaster, the s-LNvs transmit circadian information to the subsets of other clock neurons located in the dorsal protocerebrum of the brain. We examined the synaptic connections of the PDF-positive fibers of s-LNvs in the dorsal protocerebrum, and found that the fibers of s-LNvs contain large dense-cored vesicles (DCVs), as well as numerous small clear vesicles which form divergent output synapses onto unlab … More eled neurites. In addition, three-dimensional reconstruction revealed the presence of input synapses onto the s-LNvs fibers. These results indicate that the s-LNvs receive direct synaptic input in the dorsal protocerebrum, besides exhibiting structural features for supplying the dual outputs from the s-LNvs, either via synaptic outputs or via paracrine release of DCV contents. In P. terraenovae, we demonstrated that the fibers of Pt-PDF/LNs in the accessory medulla receive input synapses from the terminals of extraretinal photoreceptors called 'Pt-eyelets'. Furthermore, in the dorsal protocerebrum, we showed that the fibers of Pt-PDF/LNs form output synapses onto the PL neurons, which locate in the pars lateralis and are indispensable for the induction of reproductive diapause. These results indicate that Pt-PDF/LNs convey the light information detected by the Pt-eyelet to the PL neurons, and that they might play some roles in the time-measurement system and in sending processed information to the PL neurons. Our results provided the first insight into some basic characteristics of the clock's neural circuits. Less

为了揭示节奏运动活动的昼夜节律起搏器网络的接线，我们使用果蝇和原始的Terraenovae作为模型生物进行了调查，调查了昼夜节律前和后起搏器神经元后突触后神经元的接线。 P. terraenovae具有PDF（色素分散因子） - 免疫阳性神经元（此处称为PT-PDF/LNS），对应于果蝇PDF-PDF-免疫阳性S-LNV（小腹侧侧神经元），它们起到了昼夜节律的症状。因此，P。terraenovae也是一个很好的模型物种，与D. melanogaster并行。我们检查了背侧杂种中S-LNV的PDF阳性纤维的突触连接，并发现S-LNV的纤维包含大型致密囊泡（DCV），以及许多形成差异输出突触的小型透明的小蔬菜，这些小型蔬菜会引起非贴为非贴型的突触……更多的ELED神经素。此外，三维重建揭示了在S-LNVS纤维上存在输入突触。这些结果表明，除了表现出用于通过合成输出或通过DCV含量的旁分泌释放，S-LNV在背侧原脑中接收直接的突触输入。在P. terraenovae中，我们证明了附件髓质中的Pt-PDF/LN的纤维从称为“ Pt-Eyelets”的末端接收输入突触。此外，在背质脑中，我们表明，PT-PDF/LNS的纤维形成了PL神经元的突触，该元素位于Pars侧侧，对于诱导生殖性滞育而言是必不可少的。这些结果表明，PT-PDF/LNS将PT-EYET检测到的光信息传达给PL神经元，并且它们可能在时间衡量系统中扮演一些角色，并在将处理的信息发送给PL神经元中。我们的结果为时钟神经元的某些基本特征提供了第一个洞察力。较少的

项目成果

Synaptic connections between pigment-dispersing factor-immunoreactive neurons and neurons in the pars lateralis of the blow fly Protophormia terrraenovae.

色素分散因子免疫反应性神经元与绿头苍蝇 Protophormia terraenovae 外侧部神经元之间的突触连接。

• 发表时间: 2005
• 期刊: J.Comp.Neurol. 491
• 作者: Kawaguchi;S.;Shimada;S.;et al.;Kawaguchi et al.;Kouji Yasuyama;Kouji Yasuyama et al.;Kouji Yasuyama;泰山 浩司;Yoshitaka Hamanaka
• 通讯作者: Yoshitaka Hamanaka

Synaptic connections between eyelet photo- receptors and pigment dispersing factor- immunoreactive neurons of the blowfly Protophormia terraenovae.

绿头苍蝇 Protophormia terraenovae 的孔眼光受体和色素分散因子免疫反应神经元之间的突触连接。

• 发表时间: 2006
• 期刊: J. Comp. Neurol. 494(2)
• 作者: Kawaguchi;S.;Shimada;S.;et al.;Kawaguchi et al.;Kouji Yasuyama;Kouji Yasuyama et al.
• 通讯作者: Kouji Yasuyama et al.

Analysis of the synaptic connections by post- embedding immuno-electron microscopy in combination with intracellular dye injection.

通过嵌入后免疫电子显微镜结合细胞内染料注射分析突触连接。

• 发表时间: 2005
• 期刊: J. Electr. Microsc. Technol. Med. Biol. 19(1)
• 作者: Kawaguchi;S.;Shimada;S.;et al.;Kawaguchi et al.;Kouji Yasuyama;Kouji Yasuyama et al.;Kouji Yasuyama;泰山 浩司;Yoshitaka Hamanaka;Kouji Yasuyama et al.
• 通讯作者: Kouji Yasuyama et al.

Synaptic connections between eyelet photoreceptors and pigment dispersing factor-immunoreactive neurons of the blowfly Protophormia terraenovae.

苍蝇 Protophormia terraenovae 的孔眼感光器和色素分散因子免疫反应神经元之间的突触连接。

• 发表时间: 2006
• 期刊: J. Comp. Neurol. 494
• 作者: Kawaguchi;S.;Shimada;S.;et al.;Kawaguchi et al.;Kouji Yasuyama
• 通讯作者: Kouji Yasuyama

細胞内色素注入とpost-embedding免疫電顕法の併用によるシナプス結合関係の解析

使用细胞内染料注射和嵌入后免疫电子显微镜分析突触连接

• 发表时间: 2005
• 期刊: 医学生物学電子顕微鏡技術学会誌 19巻1号
• 作者: Kawaguchi;S.;Shimada;S.;et al.;Kawaguchi et al.;Kouji Yasuyama;Kouji Yasuyama et al.;Kouji Yasuyama;泰山 浩司
• 通讯作者: 泰山 浩司