Neurological and Endocrinological analysis of the output pathways of circadian clock driving eclosion rhythm in the flies, Drosophila melanogaster and Sarcophaga crassipalpis

果蝇、果蝇和巨须石蝇生物钟驱动羽化节律的输出途径的神经学和内分泌学分析

• 批准号: 22570076
• 负责人: YASUYAMA Kouji
• 金额: $ 3万
• 依托单位: Kawasaki Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22570076/
• 关键词: 羽化リズム; ハエ目昆虫; 概日時計; ペプチドニューロン; 免疫電顕法; キイロショウジョウバエ; シリアカニクバエ; 免疫組織化学; PDF; CCAP

To understand how the circadian clocks time the gate of eclosion in Drosophila melanogaster, we examined the neuronal connection focusing on the putative clock neurons, PDF-Tri neurons, that transiently occur associated with eclosion, and showed the synaptic connections between the PDF-Tri and the peptidergic (CCAP-, EH- or corazonin) neurons involved in eclosion behavior. We also analyzed the eclosion rhythms in the flesh fly Sarcophaga crassipalpis, and revealed that two mechanisms are required for determination of the gate of eclosion. One is driven by low titer of 20-hydroxyecdysone between 20 h and 16 h before eclosion, and the other is driven by the signal from the circadian clock transmitted later than 6 h before eclosion.

为了了解黑腹果蝇的生物钟如何为羽化门计时，我们检查了与羽化相关的短暂发生的假定时钟神经元 PDF-Tri 神经元的神经元连接，并显示了 PDF-Tri 和参与羽化的肽能（CCAP-、EH- 或 corazonin）神经元之间的突触连接 行为。我们还分析了肉蝇Sarcophaga crassipalpis的羽化节律，并揭示了确定羽化门需要两种机制。一种是在羽化前20小时至16小时之间由低滴度的20-羟基蜕皮酮驱动，另一种是由羽化前6小时之后传输的生物钟信号驱动。

项目成果

Blocking synaptic transmission with tetanus toxin light chain reveals modes of neurotransmission in the PDF-positive circadian clock neurons of Drosophila melanogaster

用破伤风毒素轻链阻断突触传递揭示了果蝇 PDF 阳性生物钟神经元的神经传递模式

• DOI: 10.1016/j.jinsphys.2011.06.004
• 发表时间: 2011
• 期刊: Journal of Insect Physiology
• 影响因子: 2.2
• 作者: Umezaki;Yujiro
• 通讯作者: Yujiro

Morphological analysis of neuronal connections between pigment-dispersing factor-immunoreactive neurons and pars intercerebralis neurons in the blow fly, Protophormia terraenovae.

丽蝇 Protophormia terraenovae 中色素分散因子免疫反应性神经元和脑间部神经元之间神经元连接的形态学分析。

• 发表时间: 2012
• 作者: Hase;Hiroaki
• 通讯作者: Hiroaki

Comparison of the effect of constant light on the ciecadian rhytm of white-eye mutant and wild-type blow fly Protophormia terraenovae.

恒定光对白眼突变体和野生型绿头蝇Protophormia terraenovae 蝉鸣节律的影响比较。

• 发表时间: 2011
• 期刊: Biological Rhythm Research
• 影响因子: 1.1
• 作者: Hamasaka;Y.
• 通讯作者: Y.

シリアカニクバエの概日時計はいつ羽化時刻を知らせるか

叙利亚蟹蝇的生物钟何时发出出现时间的信号？

• 发表时间: 2012
• 作者: Selcho;Mareike;松浦哲也,佐藤文彦,伊藤久仁子,一ノ瀬充行;山本美貴,志賀向子
• 通讯作者: 山本美貴,志賀向子

Morphological analysis of neuronalconnections between pigment-dispersingfactor-immunoreactive neurons and pars intercerebralis neurons in the blow fly, Protophormia terraenovae.

丽蝇 Protophormia terraenovae 中色素分散因子免疫反应性神经元和脑间部神经元之间神经元连接的形态学分析。

• 发表时间: 2012
• 作者: Hase H.;Matsumura R.;Ya suyama K.;Shiga S.
• 通讯作者: Shiga S.