Neurological and Endocrinological analysis of the output pathways of circadian clock driving eclosion rhythm in the flies, Drosophila melanogaster and Sarcophaga crassipalpis

点击翻译按钮获取中文摘要

To understand how the circadian clocks time the gate of eclosion in Drosophila melanogaster, we examined the neuronal connection focusing on the putative clock neurons, PDF-Tri neurons, that transiently occur associated with eclosion, and showed the synaptic connections between the PDF-Tri and the peptidergic (CCAP-, EH- or corazonin) neurons involved in eclosion behavior. We also analyzed the eclosion rhythms in the flesh fly Sarcophaga crassipalpis, and revealed that two mechanisms are required for determination of the gate of eclosion. One is driven by low titer of 20-hydroxyecdysone between 20 h and 16 h before eclosion, and the other is driven by the signal from the circadian clock transmitted later than 6 h before eclosion.