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Analysis of Myc-target gene, Mina53 in cells and in a body

细胞和体内 Myc 靶基因 Mina53 的分析

基本信息

批准号：
16570164
负责人：
TSUNEOKA Makoto
金额：
$ 2.37万
依托单位：
Kurume University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16570164/
关键词：
Oncogene Cancer Esophageal Cancer Testis Transcription myc mina53 プロモーター細胞増殖核小体 mimitin ミトコンドリア

项目摘要

Mina53 is a nuclear protein, which has a domain called JmjC. When the effect of Mina53 over-expression on activity of various promoters, it was found that Mina53 stimulates E2F site dependent promoter activity. Since E2F is a transcription factor, which highly influences cell proliferations, the activity of Mina53 to stimulate the E2F site dependent promoter activity appears to be involved in the control of cell proliferation by Mina53. In 2006, it was reported that JmjC domain is related to Histone demethylase activity. Then it becomes an important next question if Mina53 has the enzyme activity.We produced antibody that recognized mouse Mina53 protein, and then investigate expression pattern of Mina53 in a mouse body. It was observed the high expression of Mina53 in testis, spleen, colon, where cells are proliferating well. When we investigated the expression pattern of Mina53 in testis in detail, it was found that Mina53 is highly expressed in spermatogonia, especially in type A spe … More rmatogonia.These results suggest that Mina53 is related to cell proliferation not only in cancer but also in normal tissues.2,Myc-target genes can be classified into two classesWe cloned another Myc-target gene mimitin by the similar methods for mina53 cloning. Mimitin was found to encode Mitochondrial protein and involved in cell proliferation of esophageal squamous cell carcinoma cells. Mina53 and Mimitin are highly expressed in some human cancer tissues, and they are required for cell proliferation in some types of cells. However, it appears some types of cells do not require them for cell proliferation. These may be due to the existence of a functionally redundant protein and/or mechanism. These Myc-target genes can be classified into one group as "specufuc type". On the other hand, there are Myc-responsive genes, which appear to be generally important for all types of cells to proliferate. Inhibition of the function of one of the specific type of Myc-responsive genes may hamper only some restricted types of cells and would have fewer side effects and more potential than a general type for cancer therapy. Less

Mina 53是一种核蛋白，其具有称为JmjC的结构域。当研究Mina 53过表达对各种启动子活性的影响时，发现Mina 53刺激E2 F位点依赖性启动子活性。由于E2 F是高度影响细胞增殖的转录因子，Mina 53刺激E2 F位点依赖性启动子活性的活性似乎参与Mina 53对细胞增殖的控制。2006年有报道称JmjC结构域与组蛋白去甲基化酶活性有关。因此，Mina 53是否具有酶活性成为下一个重要的问题。我们制备了识别小鼠Mina 53蛋白的抗体，然后研究了Mina 53在小鼠体内的表达模式。在睾丸、脾脏、结肠中观察到Mina 53的高表达，这些细胞增殖良好。我们详细研究了Mina 53在睾丸中的表达模式，发现Mina 53在精原细胞中高表达，特别是在A型精子中， ...更多信息 2、Myc靶基因可分为两类我们用与mina 53相似的方法克隆了另一个Myc靶基因mimitin。Mimitin编码线粒体蛋白，参与食管鳞癌细胞增殖。Mina 53和Mimitin在某些人类癌症组织中高度表达，并且它们是某些类型细胞中细胞增殖所需的。然而，似乎某些类型的细胞不需要它们进行细胞增殖。这可能是由于存在功能冗余的蛋白质和/或机制。这些Myc靶基因可以被分类为一组“特异性类型”。另一方面，有Myc反应基因，这似乎是所有类型的细胞增殖一般重要。抑制一种特定类型的Myc反应基因的功能可能只会阻碍某些有限类型的细胞，并且比一般类型的癌症治疗具有更少的副作用和更大的潜力。少