Integration of chromosome mand linkage maps of Lotus japonicus, Miyakogusa as a model plant of legume

豆科植物模型植物宫古草的染色体和连锁图谱的整合

• 批准号: 16580003
• 负责人: OHMIDO Nobuko
• 金额: $ 1.86万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16580003/
• 关键词: Chromosome map; Gene Mapping; Legume; Lotus japonicus; Miyakogusa; マッピング; TACクローン; 画像解析

Lotus japonicus, Miyakojima MG-20 is a cytological model plant of the legume family. I have developed quantitative pachytene chromosome maps and combined them with the other Lotus chromosome maps to integrate their information for effective and accurate genome analysis. Pachytene chromosomes applied the quantitative chromosome image analysis and FISH methods using four repetitive sequences and 28 transformation-competent artificial chromosome (TAC) clones are used for higher resolution mapping. The addresses of gene loci had been identified by chromosome image analysis system (CHIAS), which measures the condensation pattern (CP) of the pachytene chromosome. We have integrated three maps ; mitotic prometaphase chromosome map, meiotic pachytene chromosome map and linkage map. The integrated maps demonstrated precise physical positions of TAC clones on chromosomes, and associated with chromosomal higher structures, representing in centromeres, telomeres, cytologically specific heterochromatin and euchromatin. Moreover, we compared genetic distances derived from recombination frequency with physical distances between DNA markers on pachytene chromosome maps.

莲花，宫古岛MG-20，是豆科植物的细胞学模式植物。我已经开发了定量粗线期染色体图谱，并将它们与其他莲花染色体图谱相结合，以整合它们的信息，以进行有效和准确的基因组分析。利用4个重复序列和28个具有转化能力的人工染色体(TAC)克隆，应用定量染色体图像分析和FISH方法对粗线期染色体进行高分辨率定位。用染色体图像分析系统(CHIAS)测定粗线期染色体的凝集模式(CP)，确定基因座的位置。我们已经整合了三个图谱：有丝分裂前中期染色体图谱、减数分裂粗线染色体图谱和连锁图谱。整合图谱显示了TAC克隆在染色体上的精确物理位置，并与着丝粒、端粒、细胞学特异的异染色质和常染色质的高级结构相关联。此外，我们还比较了重组频率得出的遗传距离与粗线期染色体图谱上DNA标记之间的物理距离。

项目成果

Comparison of Surface Structures between Extended and Condensed Stages of Barley Chromosome Revealed with Atomic Force Microscopy

原子力显微镜揭示大麦染色体延伸阶段和凝聚阶段表面结构的比较

• 发表时间: 2005
• 期刊: Cytologia 第70巻・第1号
• 作者: Youzhi;Ma;Ma Y.;Ohmido N.
• 通讯作者: Ohmido N.

Fluorescent labeling of plant chromosomes in suspension by FISH

• DOI: 10.1266/ggs.80.35
• 发表时间: 2005-02-01
• 期刊: GENES & GENETIC SYSTEMS
• 影响因子: 1.1
• 作者: Ma, YZ;Lee, JH;Fukui, K
• 通讯作者: Fukui, K

Application of the Bio-Active Beads Method in Rice Transformation

生物活性珠法在水稻转化中的应用

• 发表时间: 2004
• 期刊: Plant Biotechnology 7
• 作者: Suzuki;Y. et al.;Hen Lin
• 通讯作者: Hen Lin

Advances in Rice Genetics (Recent advances in FISH analysis of plant chromosomes.)

水稻遗传学进展（植物染色体 FISH 分析的最新进展。）

• 发表时间: 2004
• 作者: Ohmido;N.;Fukui;K.
• 通讯作者: K.

蛋白質 核酸 酵素

蛋白质核酸酶

• 发表时间: 2007
• 作者: 島津忠広;堀之内末治;吉田 稔
• 通讯作者: 吉田 稔