Integration of chromosome maps of allogamous diploid red clover.

同种异体二倍体红三叶草染色体图谱的整合。

• 批准号: 20580006
• 负责人: OHMIDO Nobuko
• 金额: $ 2.5万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20580006/
• 关键词: マメ科植物; 染色体地図; 遺伝子地図; FISH; 画像解析; アカクローバ; ゲノム解析

Red clover (Trifolium pratense L.) is a forage legume, an allogamous diploid (2n=2x=14) with 440Mb genome size. Seven prometaphase chromosomes were identified by fluorescence in situ hybridization (FISH) using 26S and 5S rDNA probes and bacterial artificial chromosome (BAC) clones and chromosome image analysis system ver.4.0 (CHIAS4). 14 BAC clones located close to the both end of each linkage group (LG), representatively LG1-7, exclusively hybridized to the distal regions of Chromosome 4, 2, 6, 5, 1, 7 and 3, respectively. Chromosomes images of FISH mapping were quantitatively analyzed based on CHIAS4 and construct of quantitative idiogram. This is the first complete chromosome map of red clover and integration of the genetic linkage maps and the quantitative chromosome maps provides valuable information on the genetic data and insight into the legumes genetics. This study should contribute to provide significant data for DNA markers and molecular breeding in legume varieties.

红三叶草 (Trifolium pratense L.) 是一种饲料豆科植物，是一种同源二倍体 (2n=2x=14)，基因组大小为 440Mb。使用 26S 和 5S rDNA 探针、细菌人工染色体 (BAC) 克隆和染色体图像分析系统 4.0 版 (CHIAS4)，通过荧光原位杂交 (FISH) 鉴定了 7 条中期染色体。 14个BAC克隆位于靠近每个连锁群(LG)的两端，代表性的是LG1-7，分别与染色体4、2、6、5、1、7和3的远端区域特异性杂交。基于CHIAS4和定量图的构建对FISH作图的染色体图像进行定量分析。这是第一个完整的红三叶草染色体图谱，遗传连锁图谱和定量染色体图谱的整合提供了有关遗传数据的宝贵信息和对豆科植物遗传学的深入了解。这项研究应该有助于为豆类品种的 DNA 标记和分子育种提供重要数据。

项目成果

Genome structure of the legume, Lotus japonicus.

• DOI: 10.1093/dnares/dsn008
• 发表时间: 2008-08
• 期刊: DNA research : an international journal for rapid publication of reports on genes and genomes
• 作者: Sato S;Nakamura Y;Kaneko T;Asamizu E;Kato T;Nakao M;Sasamoto S;Watanabe A;Ono A;Kawashima K;Fujishiro T;Katoh M;Kohara M;Kishida Y;Minami C;Nakayama S;Nakazaki N;Shimizu Y;Shinpo S;Takahashi C;Wada T;Yamada M;Ohmido N;Hayashi M;Fukui K;Baba T;Nakamichi T;Mori H;Tabata S
• 通讯作者: Tabata S

High-resolution FISH for gene mapping and molecular analysis of plant chromosomes.

用于植物染色体基因定位和分子分析的高分辨率 FISH。

• 发表时间: 2008

Nuclear organization analyzed by visualization.

通过可视化分析核组织。

• 发表时间: 2008
• 期刊: The Nucleus 50
• 作者: Ohmido N;Wako T;Fukui K.
• 通讯作者: Fukui K.

Sequence analysis of the genome of an oil-bearing tree, Jatropha curcas L.

• DOI: 10.1093/dnares/dsq030
• 发表时间: 2011-02
• 期刊: DNA research : an international journal for rapid publication of reports on genes and genomes
• 作者: Sato S;Hirakawa H;Isobe S;Fukai E;Watanabe A;Kato M;Kawashima K;Minami C;Muraki A;Nakazaki N;Takahashi C;Nakayama S;Kishida Y;Kohara M;Yamada M;Tsuruoka H;Sasamoto S;Tabata S;Aizu T;Toyoda A;Shin-i T;Minakuchi Y;Kohara Y;Fujiyama A;Tsuchimoto S;Kajiyama S;Makigano E;Ohmido N;Shibagaki N;Cartagena JA;Wada N;Kohinata T;Atefeh A;Yuasa S;Matsunaga S;Fukui K
• 通讯作者: Fukui K

Comprehensive chromosome map of red clover (Trifolium pratense L. ).

红三叶草（Trifolium pratense L.）的综合染色体图谱。

• 发表时间: 2008
• 作者: Kataoka R;Hara M;Kato S;Isobe S;Sato S;Tabata S;Ohmido N.
• 通讯作者: Ohmido N.