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REGULATION OF TUBEROUS ROOT FORMATION BY D-TYPE CYCLIN IN SWEET POTATO

D型细胞周期素对甘薯块根形成的调控

基本信息

批准号：
16580012
负责人：
SAITOU Kazuyuki
金额：
$ 2.43万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16580012/
关键词：
SWEET POTATO CYCLIN D TUBEROUS ROOT FORMATION SUCROSE CYTOKININ ABA アブシジン酸

项目摘要

Two cDNAs for D3-type cyclins were cloned and designated Ipoba;CycD3;1 and Ipoba;CycD3;2. The deduced amino acid sequences for Ipoba;CycD3;1 and Ipoba;CycD3;2 contained a retinoblastoma-binding motif and a cyclin box, whereas they shared only 49% identity. In the process of tuberous root formation, Ipoba;CycD3;1 was expressed in thick roots and tuberous roots at the highest level. The Ipoba;CycD3;2 gene expression reached a maximum level in thick roots. The transcript level of ADP-glucose pyrophosphorylase large subunit iAGPLI-1 increased continuously. In order to clarify the effect of sucrose and plant hormones on gene expression more precisely, leaf-petiole cuttings were used. The expression of Ipoba;CycD3;1 increased stepwise by application of 50 mM and 100 mM sucrose solution, and the highest expression level was obtained with 100 mM and 150 mM sucrose. A hundred mM was optimal for maximum expression of Ipoba;CycD3;2. The expression of iAGPLI-1 gene kept an increasing tendency with … More an increase in sucrose concentration up to 150 mM. Benzyladenine induced the expression of Ipoba;CycD3;1 and enhanced sucrose induction of Ipoba;CycD3;1 expression. The relative amount of Ipoba;CycD3;2 mRNA was slightly reduced by benzyladenine, while the transcripts increased in response to benzyladenine when sucrose was supplemented. The induction of iAGPLI-1 by sucrose was repressed by benzyladenine. ABA reduced the relative amount of Ipoba;CycD3;1 mRNA. Sucrose antagonized the inhibitory effect of ABA for Ipoba;CycD3;1 gene expression. The expression of Ipoba;CycD3;2 was not influenced by ABA. iAGPLI-1 gene expression was not significantly affected by ABA, whereas the induction of iAGPLI-1 by sucrose was inhibited by ABA. These results suggested that cell proliferation and storage parenchyma differetiation in the process of tuberous root formation were controlled via regulation of D3 cyclins and ADP-glucose pyrophosphorylase gene expression by alterations in the concentration of sucrose, cytokinin and ABA. Less

克隆了两个D3型细胞周期蛋白的cDNA，并命名为Ipoba; CycD 3;1和Ipoba; CycD 3;2。推导的氨基酸序列Ipoba; CycD 3;1和Ipoba; CycD 3;2含有视网膜母细胞瘤结合基序和细胞周期蛋白盒，而它们只有49%的同一性。在块根形成过程中，Ipoba; CycD 3;1在粗根和块根中表达量最高。Ipoba; CycD 3;2基因在粗根中表达量最高。ADP-葡萄糖焦磷酸化酶大亚基iAGPLI-1的转录水平持续升高。为了更准确地阐明蔗糖和植物激素对基因表达的影响，采用叶柄插条。Ipoba的表达; CycD3; 1通过应用50 mM和100 mM蔗糖溶液逐步增加，并且在100 mM和150 mM蔗糖下获得最高表达水平。对于Ipoba; CycD 3;2的最大表达，100 mM是最佳的。iAGPLI-1基因的表达量随时间的延长呈上升趋势， ...更多信息蔗糖浓度增加至150 mM。苄基腺嘌呤诱导Ipoba; CycD 3;1的表达，并增强Ipoba; CycD 3;1表达的蔗糖诱导。Ipoba的相对量; CycD3; 2 mRNA略有下降，苄基腺嘌呤，而成绩单增加响应苄基腺嘌呤时，补充蔗糖。蔗糖诱导的iAGPLI-1被苄基腺嘌呤抑制。阿坝降低了Ipoba; CycD 3;1 mRNA的相对量。蔗糖可拮抗阿坝对Ipoba; CycD 3;1基因表达的抑制作用。阿坝对Ipoba; CycD 3;2的表达无影响。阿坝对iAGPLI-1基因的表达没有显著影响，而阿坝抑制蔗糖诱导的iAGPLI-1基因的表达。这些结果表明，在块根形成过程中，细胞增殖和贮藏薄壁组织的再生是通过改变蔗糖、细胞分裂素和阿坝的浓度来调节D3细胞周期蛋白和ADP-葡萄糖焦磷酸化酶基因的表达而实现的。少