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Ecological Research for a new invaded termite pest, Reticulitermes kanmonensis.

新入侵白蚁害虫关门散白蚁的生态研究。

基本信息

批准号：
16580039
负责人：
TAKEMATSU Yoko
金额：
$ 2.37万
依托单位：
Yamaguchi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16580039/
关键词：
R.kanmonensis invaded species ecological features distribution nestmate recognition R.speratus aquaporin 水運搬能力

项目摘要

In this study, ecological features of a new invaded termite species, Reticulitermes kanmonensis, were investigated as below :1. Ecological features of R.kanmonensisIn the field, R.speratus and R.kamnonensis nested almost in the same damaged-level woods when they distributed allopatrically. However, R.kanmonensis nested in the lower damaged-level wood than R.speratus when they distributed sympatrically. It is also remarkable that R.kanmonensis showed the high ability of gallery construction and tolerance against dryness. In the experimental condition, the consumption rate of R.kanmonensis was highest at 30℃. This is almost same as that of R. speratus.2. Inter-and intra-species interaction among co-existent speciesR.speratus showed strong agonistic behavior against different species and no trophallactic contact. However agonistic behavior against different colony of the same species was weak and trophallactic exchange of food was observed. On the other hand, R.kanmonensis showed strong agonistic behavior against different species and different colony of the same species and trophallaxis contact was absent. These results suggested that R.speratus has colony fusion as already known, although R.kanmonensis does not show colony fusion and strongly exclude non-nestmate individuals.It is suggested that the difference in distribution patterns of the two species is due to the difference in their nestmate recognition characteristics.3. Elucidation of water utilization ability and the physiological mechanismsWe cloned and sequenced cDNAs encoding water channel protein, aquaporin (AQP) from digestive tracts of Coptotermes formosanus, in order to clarify the ability of water utilization of termite. From these results, there are at least two kinds of AQP mRNAs which can be distinguished by nucleic acid sequences since C-terminal of coding region, and the deduced amino acid sequences have 53-65% identities with presently known insect AQPs.

本研究对一种新入侵白蚁Reticulitermes kanmonensis的生态特征进行了如下研究： 1．关门杜鹃的生态特征在田间，斯佩拉图斯和关门杜鹃在异域分布时，几乎在同一受损层树林中筑巢。然而，当它们同域分布时，R.kanmonensis 的筑巢位置比 R.speratus 低。值得注意的是，R.kanmonensis 表现出很高的廊道建设能力和耐干燥能力。实验条件下，30℃时坎门杜鹃的消耗率最高。这与R. speratus 几乎相同。2。共存物种R.speratus之间的种间和种内相互作用对不同物种表现出强烈的竞争行为，并且没有营养接触。然而，对同一物种的不同群体的竞争行为较弱，并且观察到食物的营养交换。另一方面，R.kanmonensis 对不同物种和同一物种的不同群体表现出强烈的竞争行为，并且缺乏营养轴接触。这些结果表明，R.speratus 具有已知的群体融合，而 R.kanmonensis 没有表现出群体融合，并且强烈排除非同巢个体。这表明两个物种分布模式的差异是由于它们的同巢识别特征的差异所致。 3．阐明白蚁水分利用能力及生理机制我们从家白蚁消化道中克隆了编码水通道蛋白水通道蛋白(AQP)的cDNA并测序，以阐明白蚁的水分利用能力。从这些结果来看，至少有两种AQP mRNA可以通过从编码区C端开始的核酸序列来区分，并且推导的氨基酸序列与目前已知的昆虫AQP有53-65％的同一性。