Improvement in ozone tolerance of poplar plants with an antisense and sense DNA for 1-aminocyclopropane-1-carboxylate synthase

在花椰菜花叶病毒35S启动子的控制下，将臭氧诱导的杨树1-氨基环丙烷-1-羧酸(ACC)合成酶的反义和正义DNA导入杨树，获得转基因植株。在20株转基因植物中，有15株观察到臭氧诱导的乙烯生成率低于野生型植物。在这15个品系中，臭氧诱导的可见伤害都有所减弱，且伤害的程度与臭氧诱导的乙烯产生水平呈正相关。另一方面，在10株转基因植物中，有4株观察到臭氧诱导的乙烯生成率高于野生型植物。转基因植株和野生型植株在生长和形态上没有显著差异。转基因植株在臭氧胁迫下的气孔导度高于野生型植株。这些发现表明，为臭氧诱导的ACC合成酶引入反义和正义DNA可以在不降低植物的气体吸收和生产力的情况下提高植物的臭氧耐受性。

Antisense and sense DNA for an ozone-inducible 1-aminocyclopropane-1-carboxylate (ACC) synthase from poplar, under the control of the cauliflower mosaic virus 35S promoter, was introduced into poplar to generate transgenic plants. Lower rates of ozone-induced ethylene production were observed in fifteen of twenty transgenic plants than in the wild-type plants. Ozone-induced visible damage was attenuated in these fifteen lines, and the extent of damage was positively related to the level of ozone-induced ethylene production. On the other hand higher rates of ozone-induced ethylene production were observed in four of ten transgenic plants than in the wild-type plants. No significant differences in growth and morphology were observed between transgenic and wild-type plants. Stomatal conductance of transgenic plants during ozone exposure was higher than that of wild-type plants. These findings indicate that the introduction of antisense and sense DNA for an ozone-inducible ACC synthase can improve the ozone tolerance of plants without reducing their gas absorption and productivity.