Studies on osmoregulation by neurosecretory system in crustaceans

甲壳动物神经分泌系统渗透压调节的研究

• 批准号: 16580171
• 负责人: YASUDA Akikazu
• 金额: $ 2.3万
• 依托单位: Suntory Institute for Bioorganic Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16580171/
• 关键词: crayfish; neuropeptide; osmoregulation; anal respiration; orcokinin; tachykinin-related peptide; crustacean cardioactive peptide; crustacean-SIFamide; マイオサプレッシン; SIFアミド; 腹部神経節; CCAP; MALDI-TOF MS; 甲殻類心臓作用性ペプチド; アラトスタチン関連ペプチド; FLRFアミド関連ペプチド

To investigate osmoregulation by the central nerve system in crustaceans, topological mass spectrometry analysis was available for the identification of neuropeptides. In the crayfish (Procambarus clarkii), "anal respiration" has been described in the book "The Crayfish : An Introduction to the Study of Zoology" published in 1880. The observation has been noted that water is alternately taken into and expelled from the anus fifteen to seventeen times in a minute and it seemed to be regulated by the sixth abdominal ganglion. Direct matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry analysis with slice of the sixth abdominal ganglion was resulted in the presence of orcokinin and its gene-related peptides, crustacean-SIFamide, decapod tachykinin-related peptide, crustacean cardioactive peptide, myosupressin-like peptide. These peptides were examined in vivo on bioassays for rhythmic anal contraction and anal water flux. The activity of the peptides on the is … More olated intestine was also examined in vitro. Rhythmic anal contraction was caused by orcokinins, decapod tachykinin-related peptide, when each sample of 100 pmol was injected into the crayfish. Crustacean cardioactive peptide had most potent activity of anal opening. Water flux through the anus was observed by injection of crustacean cardioactive peptide or decapod tachykinin-related peptide. Interestingly, the action of the neuropeptides on the isolated intestine was diversified. The contraction of hindgut was activated by orcokinins, but not by crustacean cardioactive peptide or myosupressin-like peptide. Decapod tachykinin-related peptide showed repetitive action alternating between contraction and relaxation on the rectum. Crustacean-SIFamide caused complex movements of the hindgut, and induced most potent activity of defecation. We concluded that the crayfish can take water from the anus, but each function of neuropeptides identified in sixth abdominal ganglion remains unclear. We also think that "anal respiration" might not be a normal phenomenon, since the action was able to be reproduced by the overdose of a combination with crustacean-SIFamide and decapod tachykinin-related peptide. Less

为了研究甲壳类中枢神经系统的渗透调节作用，采用拓扑质谱联用技术对神经肽进行了鉴定。在克氏原虾(Procambarus Clarkii)中，《小龙虾：动物学研究导论》一书在1880年出版时曾描述过肛门呼吸。观察到，肛门内的水被交替地吸收和排出15到17次，似乎是由第六腹神经节调节的。采用基质辅助激光解吸电离飞行时间质谱仪对第六腹神经节切片进行直接基质辅助激光解吸/电离飞行时间质谱分析，结果发现甲壳动物SIF酰胺、十足类速激肽、甲壳动物心脏活性多肽、肌压素样多肽等基因相关多肽的存在。这些多肽在体内进行了生物测定，以检测肛门节律性收缩和肛门水流量。多肽在IS…上的活性在体外也检查了更多的受试者的肠管。当向小龙虾中注入每个100pmoL的样本时，由Orcokinins引起的节律性肛门收缩是由十足速激肽相关的多肽引起的。甲壳动物心脏活性多肽具有最强的肛门开放活性。通过注射甲壳动物心脏活性多肽或十足速激肽相关多肽观察肛门水流量。有趣的是，神经肽在离体肠上的作用是多样化的。后肠的收缩可被或可激肽激活，但不能被甲壳类心脏活性多肽或肌压素样多肽激活。十足类速激肽相关肽在直肠的收缩和松弛之间显示出重复的作用。甲壳动物-SIF酰胺引起复杂的后肠运动，并诱导最有效的排便活动。我们的结论是，小龙虾可以从肛门摄取水分，但第六腹神经节中发现的神经肽的每一种功能尚不清楚。我们还认为“肛门呼吸”可能不是一种正常现象，因为这种作用可以通过过量甲壳类动物-SIF酰胺和十足类速激肽相关肽的组合来复制。较少

项目成果

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• 发表时间: 2006
• 期刊: Gen. Comp. Endocrinol. 148
• 作者: Takahashi;A.
• 通讯作者: A.

化学と生物

化学和生物学

• 发表时间: 2007
• 作者: Tsuji Y;Watanabe K;Araki K;Shinohara M;Yamagata Y;Tsurimoto T;Hanaoka F;Yamamura K;Yamaizumi M;Tateishi;S.;清成信一
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APSGFLGMRamide is a unique tachykinin-related peptide in crustaceans.

APSGFLGMRamide 是甲壳类动物中独特的速激肽相关肽。

• 发表时间: 2004
• 期刊: Eur. J. Biochem. 271
• 作者: Yasuda-Kamatani;Y.;Yasuda;A
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Nucleotide sequence and expression of three subtypes of proopiomelanocortin mRNA in barfin flounder.

• DOI: 10.1016/j.ygcen.2005.01.010
• 发表时间: 2005-05
• 期刊: General and comparative endocrinology
• 影响因子: 2.7
• 作者: A. Takahashi;M. Amano;T. Itoh;A. Yasuda;Takeshi Yamanome;Y. Amemiya;K. Sasaki;M. Sakai;K. Yamamor

新規生理活性ペプチドの新しい探索法

新型生物活性肽的新搜索方法

• 发表时间: 2004
• 期刊: 化学と生物 42
• 作者: 安田明和;安田好美
• 通讯作者: 安田好美