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Development of liposomal intranasal vaccine for prevention of mastitis in cattle.

开发用于预防牛乳腺炎的脂质体鼻内疫苗。

基本信息

批准号：
16580255
负责人：
WATARAI Shinobu
金额：
$ 2.37万
依托单位：
Osaka Prefecture University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16580255/
关键词：
Intranasal vaccine Liposome Mastitis Cattle Vaccine IgA

项目摘要

The usefulness of fusiogenic liposomes composed of DPPC (1mmol), DPPS (1mmol), and SucPG (0.7mg) (SucPG-liposome) as vehicle for the antigen delivery system in the induction of a protective immune response against bovine mastitis was evaluated. Mice were immunized with OVA-containing SucPG-liposome intranasally. After immunization, significantly higher antigen-specific IgG and IgA antibodies were detected in sera than in the non-immunized control group. The usefulness of adsorptive SucPG-liposome having chitosan (Chito-SucPG-liposome) for the induction of the immune responses was also examined. After intranasal administration of OVA-containing Chito-SucPG-liposome, further augmentation of IgG and IgA antibody responses against OVA was observed. When sera from immunized mice with SucPG-liposome and Chito-SucPG-liposome were analyzed for isotype distribution, antigen-specific IgG1, IgG2a, and IgG3 antibody responses were noted. This result suggests that both humoral (Th2-type) and cell-mediated (Th1-type) immunity are induced by intranasal immunization with SucPG-liposome- and Chito-SucPG-liposome-associated antigens.In addition, the immune responses to antigen entrapped in Chito-SucPG-liposome were examined by intranasal immunization of cattle with OVA-containing Chito-SucPG-liposome. The intranasal administration with OVA-containing Chito-SucPG-liposome induced significant IgG and IgA responses to OVA in sera and milk from all of cattle administered. This data demonstrates that Chito-SucPG-liposome would have the potential usefulness for the induction of a protective immune response against bovine mastitis and suggests the possibility of developing vaccines that may ultimately be used for the prevention of mastitis in cattle.

评价了由DPPC(1 Mmol)、DPPS(1 Mmol)和SucPG(0.7 mg)(SucPG-脂质体)组成的融合脂质体作为抗原递送系统的载体在诱导对牛乳房炎的保护性免疫反应中的有效性。用含OVA的SucPG脂质体鼻腔免疫小鼠。免疫后血清中抗原特异性Ig G和Ig A抗体水平显著高于未免疫对照组。此外，还考察了壳聚糖吸附SucPG脂质体(Chito-SucPG-Liposome)诱导免疫应答的有效性。鼻腔注射含OVA的壳聚糖脂质体后，可进一步增强小鼠抗OVA的抗体应答。对SucPG-脂质体和Chito-SucPG-脂质体免疫小鼠的血清进行同型分布分析时，发现了抗原特异性的IgG1、IgG2a和IgG3抗体应答。这一结果表明，SucPG脂质体和Chito-SucPG脂质体相关抗原滴鼻免疫牛均能诱导体液免疫(Th2型)和细胞免疫(Th1型)。此外，用含OVA的Chito-SucPG脂质体对牛进行鼻腔免疫，观察其对Chito-SucPG脂质体抗原的免疫应答。鼻腔注射含OVA的壳聚糖脂质体后，所有牛的血清和乳汁对OVA均有明显的免疫球蛋白和免疫球蛋白A反应。这一数据表明，几丁糖-葡聚糖脂质体有可能用于诱导对牛乳房炎的保护性免疫反应，并表明开发最终可用于预防牛乳房炎的疫苗的可能性。