权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Plant Metabolomics Research on a basis of Fourier Transform Ion Cyclotron Mass Spectrometry

基于傅里叶变换离子回旋质谱的植物代谢组学研究

基本信息

批准号：
16580281
负责人：
OHTA Daisaku
金额：
$ 2.3万
依托单位：
Osaka Prefecture University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16580281/
关键词：
Metabolomics P450 FT-ICR MS Fourier Transform Ion Cyclotron Mass Spectrometry 質量分析 FT-MS 多変量解析

项目摘要

We have developed a metabolic profiling scheme based on a direct-infusion Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR MS). The scheme consists of 1)reproducible data collection under optimized FT-ICR MS analytical conditions, 2)automatic mass-error correction and multivariate analyses for metabolome characterization using a newly developed metabolomics tool (Dmass software), 3)identification of marker metabolites by searching an in-house metabolite database, KNApSAcK, and 4)structural analyses by an MSMS method. The scheme was applied for clarification of metabolic disorders in Arabidopsis seedlings treated with different herbicidal chemical classes. Arabidopsis extracts were directly infused into an electrospray ionization source on an FT-ICR MS system. Acquired metabolomics data consisting of m/z values with ion intensity information were subjected to a principal component analysis, and disordered metabolomes in the herbicide treatments were clearly differentiated from an herbicide-free metabolome. From each herbicide treatment, candidate metabolites representing the metabolic disorders were identified through the KNApSAcK database search. The database search and MSMS analyses manifested dose dependent accumulation patterns of specific metabolites including several flavonoid glycosides. The metabolic profiling scheme with FT-ICR MS and the Dmass program is discussed as a chemical genomics tool.

我们开发了一种基于直接注入傅立叶变换离子回旋共振质谱（FT-ICR MS）的代谢分析方案。该方案包括1）在优化的FT-ICR MS分析条件下收集可重现的数据，2）使用新开发的代谢组学工具（Dmass软件）进行代谢组学表征的自动质量误差校正和多变量分析，3）通过搜索内部代谢物数据库KNApSAcK鉴定标记代谢物，以及4）通过MSMS方法进行结构分析。该方案适用于澄清代谢紊乱拟南芥幼苗处理不同的除草化学类。拟南芥提取物直接注入FT-ICR MS系统上的电喷雾电离源。所获得的代谢组学数据组成的m/z值与离子强度信息进行了主成分分析，并在除草剂治疗中的紊乱代谢组明显区别于无除草剂的代谢组。通过KNApSAcK数据库检索，从每种除草剂处理中鉴定代表代谢紊乱的候选代谢物。数据库检索和MSMS分析显示特定代谢物（包括几种黄酮苷）的剂量依赖性蓄积模式。与FT-ICR MS和Dmass程序的代谢分析方案作为化学基因组学工具进行了讨论。