Applied metabolomics based on the Fourie transform ion cyclotron mass spectrometry

借助傅里叶变换离子回旋共振质谱（FT-ICR/MS）的极高分辨率，我们开发了一个代谢组学平台，用于高通量代谢谱分析和代谢物候选物鉴定，该平台集成了数据处理系统Dr.DMASS程序（http：//kanaya.naist.jp/DrDMASS/）和代谢物物种数据库KNApSAcK（http：//kanaya.aist-nara.ac.jp/KNApSAcK/）。整个方案被应用于研究关键的代谢事件和因此涉及的酶。首先，这FT-ICR/MS为基础的代谢分析方案被用来澄清植物代谢紊乱和特定的代谢物积累模式所造成的除草剂酶抑制剂。此外，该方案被应用于区分代谢活动，通过详细比较代谢物积累模式，反映光/暗条件下，在拟南芥T87细胞培养。此外，一些植物细胞色素P450的功能已被证明使用FT-ICR/MS代谢组学研究。

With the aid of the extreme resolving power of Fourier-transform ion-cyclotron-resonance mass spectrometry (FT-ICR/MS), we have developed a metabolomics platform for high-throughput metabolic profiling and metabolite candidate identification integrating a data-processing system, the Dr.DMASS program (http://kanaya.naist.jp/DrDMASS/), and a metabolite-species database, KNApSAcK (http://kanaya.aist-nara.ac.jp/KNApSAcK/). The entire scheme was applied to study key metabolic events and enzymes involved therefore. First, this FT-ICR/MS-based metabolic profiling scheme was used to clarity plant metabolic disorders and specific metabolite accumulation patterns caused by herbicidal enzyme inhibitors. Also, the scheme was applied to differentiate metabolic activities through detailed comparison of metabolite accumulation patterns reflecting light/dark conditions in Arabidopsis T87 cell culture. Furthermore, several plant cytochromes P450 functions have been demonstrated using the FT-ICR/MS based metabolomics studies.