Applied metabolomics based on the Fourie transform ion cyclotron mass spectrometry

基于傅里叶变换离子回旋质谱的应用代谢组学

• 批准号: 18380201
• 负责人: OHTA Daisaku
• 金额: $ 8.74万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18380201/
• 关键词: FT-ICR; MS; metabolomics; Arabidopsis; metabolic profiling; metabolic phenotyping; cytochrome P450; 酵素反応解析; P450; 有用物質生産

With the aid of the extreme resolving power of Fourier-transform ion-cyclotron-resonance mass spectrometry (FT-ICR/MS), we have developed a metabolomics platform for high-throughput metabolic profiling and metabolite candidate identification integrating a data-processing system, the Dr.DMASS program (http://kanaya.naist.jp/DrDMASS/), and a metabolite-species database, KNApSAcK (http://kanaya.aist-nara.ac.jp/KNApSAcK/). The entire scheme was applied to study key metabolic events and enzymes involved therefore. First, this FT-ICR/MS-based metabolic profiling scheme was used to clarity plant metabolic disorders and specific metabolite accumulation patterns caused by herbicidal enzyme inhibitors. Also, the scheme was applied to differentiate metabolic activities through detailed comparison of metabolite accumulation patterns reflecting light/dark conditions in Arabidopsis T87 cell culture. Furthermore, several plant cytochromes P450 functions have been demonstrated using the FT-ICR/MS based metabolomics studies.

借助傅里叶变换离子回旋共振质谱 (FT-ICR/MS) 的极高分辨能力，我们开发了一个用于高通量代谢分析和候选代谢物识别的代谢组学平台，集成了数据处理系统、Dr.DMASS 程序 (http://kanaya.naist.jp/DrDMASS/) 和 代谢物种类数据库，KNApSAcK (http://kanaya.aist-nara.ac.jp/KNApSAcK/)。整个方案用于研究关键的代谢事件和与之相关的酶。首先，这种基于 FT-ICR/MS 的代谢分析方案用于阐明除草酶抑制剂引起的植物代谢紊乱和特定代谢物积累模式。此外，该方案还通过详细比较拟南芥 T87 细胞培养物中反映光/暗条件的代谢物积累模式来区分代谢活动。此外，基于 FT-ICR/MS 的代谢组学研究已经证明了几种植物细胞色素 P450 的功能。

项目成果

Differential metabolomics unraveling light/dark regulation of metabolic activities in Arabidopsis cell culture

• DOI: 10.1007/s00425-007-0594-z
• 发表时间: 2007-12-01
• 期刊: PLANTA
• 影响因子: 4.3
• 作者: Nakamura, Yukiko;Kimura, Atsuko;Ohta, Daisaku
• 通讯作者: Ohta, Daisaku

Cytochromes P450 in Plant Sterol Biosynthesis

植物甾醇生物合成中的细胞色素 P450

• 作者: Daisaku;Ohta
• 通讯作者: Ohta

シトクロムCYP710Aサブファミリー遺伝子の発現解析

细胞色素CYP710A亚家族基因的表达分析

• 发表时间: 2008
• 作者: 野山 晋平;瑳峨 寛久;太田 大策
• 通讯作者: 太田 大策

ヒメツリガネゴケにおける桂皮酸モノリグノール経路遺伝子HCTの同定

立碗藓中肉桂酸单体木质素途径基因HCT的鉴定

• 发表时间: 2008
• 作者: 辰巳 舞;甲斐 光輔;太田 大策
• 通讯作者: 太田 大策

Identification and characterization of CYP710A as the sterol C-22 desaturase in plants

植物中甾醇 C-22 去饱和酶 CYP710A 的鉴定和表征

• 作者: Daisaku;Ohta;Masaharu;Mizutani;Tomomi;Morikawa
• 通讯作者: Morikawa