Applied metabolomics based on the Fourie transform ion cyclotron mass spectrometry

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With the aid of the extreme resolving power of Fourier-transform ion-cyclotron-resonance mass spectrometry (FT-ICR/MS), we have developed a metabolomics platform for high-throughput metabolic profiling and metabolite candidate identification integrating a data-processing system, the Dr.DMASS program (http://kanaya.naist.jp/DrDMASS/), and a metabolite-species database, KNApSAcK (http://kanaya.aist-nara.ac.jp/KNApSAcK/). The entire scheme was applied to study key metabolic events and enzymes involved therefore. First, this FT-ICR/MS-based metabolic profiling scheme was used to clarity plant metabolic disorders and specific metabolite accumulation patterns caused by herbicidal enzyme inhibitors. Also, the scheme was applied to differentiate metabolic activities through detailed comparison of metabolite accumulation patterns reflecting light/dark conditions in Arabidopsis T87 cell culture. Furthermore, several plant cytochromes P450 functions have been demonstrated using the FT-ICR/MS based metabolomics studies.