Study of adiponectin, structure, functions and clinical meanings.

脂联素是一种仅由脂肪细胞分泌的蛋白质，并且以至少三种形式存在于人血浆中，三聚体、六聚体和高分子量（HMW）多聚体。1）血浆脂联素浓度，每种脂联素分子的%，即，HMW，六聚体和三聚体，明胶结合脂联素比率（明胶结合脂联素/总脂联素）和HMW/总脂联素（HMW脂联素浓度/总脂联素浓度）进行分析。我们的新型ELISA使用我们的一种抗HMW脂联素的单克隆抗体，是HMW脂联素特异性的，并且利用这种ELISA，159名临床健康女性的HMW脂联素浓度为7.8±4.9 μg/mL（平均值±SD），87名临床健康男性为4.1±2.7 μg/mL（平均值±SD），即，显著降低（p<0.001）。高分子量脂联素浓度与体重、BMI、脂肪%、腰围、TG呈负相关，与高密度脂蛋白胆固醇呈正相关。 关于我们 女性血清中六聚体和三聚体的含量无显著性差异，男性血清中六聚体和三聚体的含量有显著性差异。2）对冠心病、糖尿病患者及手术后患者血清脂联素水平进行分析，发现冠心病患者血清脂联素水平及总脂联素水平均高于健康人，这可能是治疗的结果。在手术后的治疗过程中，HMW脂联素浓度的升高速度快于总脂联素，提示HMW脂联素可能是一个很好的监测指标。3）利用从人血浆中纯化的人脂联素，高分子量脂联素可抑制骨髓巨噬细胞向破骨细胞分化，诱导抗-炎性细胞因子此外，这些活动与明胶的亲和力呈正相关。少

Adiponectin is a protein exclusively secreted by adipocytes and exists in human plasma as at least three forms, i.e., a trimer, a hexamer and a high-molecular-weight (HMW) multimer.1)The plasma adiponectin concentration, % of each adiponectin molecule, i.e., HMW, the hexamer and trimer, a gelatin-binding adiponectin ratio (gelatin-binding adiponectin/total adiponectin) and HMW/Total (HMW adiponectin concentration/Total adiponectin concentration) of each individual were analyzed.Our novel ELISA using one of our monoclonal antibodies against HMW adiponectin is HMW adiponectin specific and with this ELISA, the HMW adiponectin concentrations were 7.8±4.9 μg/mL (mean±SD) in 159 clinically healthy women and 4.1±2.7 μg/mL (mean±SD) in 87 clinically healthy men, i.e., significantly lower (p<0.001). HMW concentration was negatively correlated with body weight, BMI, fat %, west size and TG, and positively with HDL-cholesterol.Although, independent of the HMW adiponectin concentration, the ratio … More of HMW, and the hexamer and trimer in women's sera did not differ greatly, those in men's sera differed greatly. The men's sera with lower HMW concentrations exhibited lower HMW% and gelatin-binding adiponectin ratios.2)The adiponectin concentrations in patients of coronary artery disease and diabetes mellitis (DM), and patients after surgical operations were analysed.Both of HMW and total adiponectin concentrations were higher in patients than healthy subjects, which might be results of treatments.By treatment of DM and hyperlipidemia, and weight reduction, and in cure process after surgical operation, HMW adiponectin concentration was increased faster than that of total adiponectin and these results suggested that HMW adiponectin might be a good monitoring parameter.3)Using human adiponectn purified from human plasma, recombinant wild-type and mutant adiponectin, biological activities were analyzed.HMW adiponectin inhibited differentiation of bone marrow macrophage to osteoclasts and induced secretion of anti-inflammatory cytokines. Moreover, these activities were positively correlated with affinity to gelatin. Less