权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Synchromization of suprachiasmatic cells

视交叉上细胞的同步化

基本信息

批准号：
16590178
负责人：
WATANABE Kazuto
金额：
$ 1.79万
依托单位：
Dokkyo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590178/
关键词：
suprachiasmatic nucleus circadian rhythm period light pulse vasonpressin VIP phase shift entrainment 培養

项目摘要

In mammals, circadian rhythms are driven by a pacemaker located in the suprachiasmatic nuclei (SCN) of the hypothalamus. The pacemaker is composed of an ensemble of multiple, single-cell oscillators in the SCN. Isolated SCN shows clear circadian oscillation in release of arginine vasopressin (AVP) in organotypic slice cultures. When the ventral part was removed by a surgical cut across the slice in the horizontal plane, the period became shorten On the other hand, the removal of the dorsal part did not affect period length. When the rostral, central and caudal SCN were cultured separately, the rostral and caudal parts of the SCN showed shorter periods than central SCN. Therefore, it is suggested that the shorter periodic region is located in a shell, a subdivision composed predominantly of AVP cells, and their rhythm is synchronized to a core region, a subdivision composed of vasoactive intestinal polypeptide (VIP) cells. Immunohistchemical analysis of the slice showed that proportions of VIP-positive region in the SCN are smaller in the slices which showed shorter periods. In the slice contains core region, furthermore, the applications of VIP antagonist shortened the periods of the AVP releasing rhythm. These data indicate that the oscillators in AVP cells have short periods and are entrained by VIP cells to form a single integrated rhythm. In slice cultures, pulse application of NM DA, an agonist of glutamate, induced light pulse-type phase shifts in AVP releasing rhythm. In dissociated cell cultures, however, VIP but not glutamate induced the phase shifts. NMDA-induced phase shifts in slice cultures were attenuated in the presence of VIP antagonist. These data indicate that photic information entrain VIP cells at first and then VIP cells entrain AVP cells. Together with all findings, it is suggested that core part of the SCN would regulate the shell part by VIP.

在哺乳动物中，昼夜节律由位于下丘脑的视交叉上核（SCN）中的起搏器驱动。起搏器由SCN中的多个单细胞振荡器的集合组成。在器官型切片培养中，分离的SCN显示精氨酸加压素（AVP）释放的明显昼夜振荡。当在水平平面上切开腹侧部分时，周期变短，而切除背侧部分对周期长度无影响。将SCN的头端、中央和尾部分别培养，SCN的头端和尾部比中央SCN的周期短。因此，有人建议，较短的周期性区域位于一个壳，主要由AVP细胞的细分，其节奏是同步的核心区域，由血管活性肠多肽（VIP）细胞的细分。切片免疫组化分析显示，周期较短的切片中，SCN内VIP阳性区域所占比例较小。在含有核心区的脑片中，VIP拮抗剂的应用缩短了AVP释放节律的周期。这些数据表明，AVP细胞中的振荡器具有短周期，并被VIP细胞夹带，形成单一的整合节律。在切片培养中，谷氨酸激动剂NMDA的脉冲应用引起AVP释放节律的光脉冲型相移。然而，在分离的细胞培养物中，VIP而不是谷氨酸诱导的相移。在VIP拮抗剂的存在下，NMDA诱导的切片培养物中的相移被减弱。说明光信息首先携带VIP细胞，然后VIP细胞携带AVP细胞。结合以上研究结果，我们认为SCN的核心部分可能通过VIP来调控壳层部分。